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Q8ET99

- SYI2_OCEIH

UniProt

Q8ET99 - SYI2_OCEIH

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Protein
Isoleucine--tRNA ligase 2
Gene
ileS2, OB0362
Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-408-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 2
Short name:
IleRS 2
Gene namesi
Name:ileS2
Ordered Locus Names:OB0362
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10221022Isoleucine--tRNA ligase 2UniRule annotation
PRO_0000098552Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB0362.

Structurei

3D structure databases

ProteinModelPortaliQ8ET99.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionUniRule annotation
Add
BLAST
Motifi582 – 5865"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ET99-1 [UniParc]FASTAAdd to Basket

« Hide

MDHAQRERSM RAHWKENRTF QRSIENREGQ QTYVFYEGPP TANGMPHAGH     50
ALGRTIKDFV ARYKTMSGYQ VLRKAGWDTH GLPVELEVEK QLNIRGKDDI 100
EKFGVERFIE KCKESVFVYE NEWKEFTDKL GYWVDMEDPY KTLNNPYIES 150
VWNILATIHD EDLLYKGHRV VPYCPNCETS LSSHEVAQGY KDVTDLSVTA 200
KFRIKNKENE YILGWTTTPW TLPGNVALAV NPEMTYVKAK QGEEIYVVAK 250
SLANTVLGEA YEVVEEVSGE DLVGINYEAP FDFISLDNGH YVIGADFVTD 300
TSGTGIVHLN PAHGEDDYNA IQAKGMDFVN VVDSKGRYED VIAPLAGQFV 350
KDSDVDIIKM LAKDGTLYEK QKYEHSYPHC WRCDTPLLYY AMEGWFIKTT 400
AVKERMQENN QSVEWFPNHM RDGRFGNFLD NMVDWNIGRN RYWGTPLNVW 450
VCDDCDKQKS PHSIQELRAF ADSDVPEDIE LHKPYVDKIT FDCECGGKMH 500
RTKEVIDVWF DSGSMPFAQY HHPFENDELF NKQFPADVVI EGVDQTRGWF 550
YSLLAVSTLF TGKTPYKRVL SLGHILDEHG RKMSKSKGNA LSPVELIEEF 600
GADALRWALL ADSSPWNNKR FSKKTVSQAK SKVIDTILNV YSFYSMYADI 650
DKFNVAEHAT DAKTTLDSWI LSRLNSVIKE VTTSLDQYDI TKGARSIALF 700
VDELSNWYVR RSRQRFWSSG MNEDKKAAFT TLHEVLSKLT QLMAPYIPFV 750
ADDIYTNLQG DSVHVSDFPK VDEAAIDEKL EADMQGVLEV IEGARSLRNE 800
VNIKTKQPLA ELVVVPNDEQ LQSGLQAYTS IIEEEINVKQ VSFKKDTADF 850
LDTEMKLNFP VAGPKLGKQV GQAKGKVENF TEQEKEAFLV NGTINVELQD 900
GSEVTLEKED IIVEKKGQEG YQLFEGNRFV LLLDTNLTEK LKEEGFVREF 950
IRAVQTYRKE LDLPVEQRVD IYVNSDENAE RILSEFDALI HDGLIINSVQ 1000
FAEKEDMKQV TVNDTEMGLS IV 1022
Length:1,022
Mass (Da):116,800
Last modified:March 1, 2003 - v1
Checksum:i4BCF597F1774244B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC12318.1.
RefSeqiNP_691283.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC12318; BAC12318; BAC12318.
GeneIDi1015648.
KEGGioih:OB0362.
PATRICi22791639. VBIOceIhe82024_0373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC12318.1 .
RefSeqi NP_691283.1. NC_004193.1.

3D structure databases

ProteinModelPortali Q8ET99.
ModBasei Search...

Protein-protein interaction databases

STRINGi 221109.OB0362.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC12318 ; BAC12318 ; BAC12318 .
GeneIDi 1015648.
KEGGi oih:OB0362.
PATRICi 22791639. VBIOceIhe82024_0373.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci OIHE221109:GI2A-408-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiSYI2_OCEIH
AccessioniPrimary (citable) accession number: Q8ET99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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