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Q8ET99

- SYI2_OCEIH

UniProt

Q8ET99 - SYI2_OCEIH

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Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-408-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 2UniRule annotation
Short name:
IleRS 2UniRule annotation
Gene namesi
Name:ileS2UniRule annotation
Ordered Locus Names:OB0362
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10221022Isoleucine--tRNA ligase 2PRO_0000098552Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB0362.

Structurei

3D structure databases

ProteinModelPortaliQ8ET99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi582 – 5865"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ET99-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDHAQRERSM RAHWKENRTF QRSIENREGQ QTYVFYEGPP TANGMPHAGH
60 70 80 90 100
ALGRTIKDFV ARYKTMSGYQ VLRKAGWDTH GLPVELEVEK QLNIRGKDDI
110 120 130 140 150
EKFGVERFIE KCKESVFVYE NEWKEFTDKL GYWVDMEDPY KTLNNPYIES
160 170 180 190 200
VWNILATIHD EDLLYKGHRV VPYCPNCETS LSSHEVAQGY KDVTDLSVTA
210 220 230 240 250
KFRIKNKENE YILGWTTTPW TLPGNVALAV NPEMTYVKAK QGEEIYVVAK
260 270 280 290 300
SLANTVLGEA YEVVEEVSGE DLVGINYEAP FDFISLDNGH YVIGADFVTD
310 320 330 340 350
TSGTGIVHLN PAHGEDDYNA IQAKGMDFVN VVDSKGRYED VIAPLAGQFV
360 370 380 390 400
KDSDVDIIKM LAKDGTLYEK QKYEHSYPHC WRCDTPLLYY AMEGWFIKTT
410 420 430 440 450
AVKERMQENN QSVEWFPNHM RDGRFGNFLD NMVDWNIGRN RYWGTPLNVW
460 470 480 490 500
VCDDCDKQKS PHSIQELRAF ADSDVPEDIE LHKPYVDKIT FDCECGGKMH
510 520 530 540 550
RTKEVIDVWF DSGSMPFAQY HHPFENDELF NKQFPADVVI EGVDQTRGWF
560 570 580 590 600
YSLLAVSTLF TGKTPYKRVL SLGHILDEHG RKMSKSKGNA LSPVELIEEF
610 620 630 640 650
GADALRWALL ADSSPWNNKR FSKKTVSQAK SKVIDTILNV YSFYSMYADI
660 670 680 690 700
DKFNVAEHAT DAKTTLDSWI LSRLNSVIKE VTTSLDQYDI TKGARSIALF
710 720 730 740 750
VDELSNWYVR RSRQRFWSSG MNEDKKAAFT TLHEVLSKLT QLMAPYIPFV
760 770 780 790 800
ADDIYTNLQG DSVHVSDFPK VDEAAIDEKL EADMQGVLEV IEGARSLRNE
810 820 830 840 850
VNIKTKQPLA ELVVVPNDEQ LQSGLQAYTS IIEEEINVKQ VSFKKDTADF
860 870 880 890 900
LDTEMKLNFP VAGPKLGKQV GQAKGKVENF TEQEKEAFLV NGTINVELQD
910 920 930 940 950
GSEVTLEKED IIVEKKGQEG YQLFEGNRFV LLLDTNLTEK LKEEGFVREF
960 970 980 990 1000
IRAVQTYRKE LDLPVEQRVD IYVNSDENAE RILSEFDALI HDGLIINSVQ
1010 1020
FAEKEDMKQV TVNDTEMGLS IV
Length:1,022
Mass (Da):116,800
Last modified:March 1, 2003 - v1
Checksum:i4BCF597F1774244B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12318.1.
RefSeqiNP_691283.1. NC_004193.1.
WP_011064767.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC12318; BAC12318; BAC12318.
GeneIDi1015648.
KEGGioih:OB0362.
PATRICi22791639. VBIOceIhe82024_0373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12318.1 .
RefSeqi NP_691283.1. NC_004193.1.
WP_011064767.1. NC_004193.1.

3D structure databases

ProteinModelPortali Q8ET99.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221109.OB0362.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC12318 ; BAC12318 ; BAC12318 .
GeneIDi 1015648.
KEGGi oih:OB0362.
PATRICi 22791639. VBIOceIhe82024_0373.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci OIHE221109:GI2A-408-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiSYI2_OCEIH
AccessioniPrimary (citable) accession number: Q8ET99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3