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Q8ET99

- SYI2_OCEIH

UniProt

Q8ET99 - SYI2_OCEIH

Protein

Isoleucine--tRNA ligase 2

Gene

ileS2

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei585 – 5851ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciOIHE221109:GI2A-408-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase 2UniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetase 2UniRule annotation
    Short name:
    IleRS 2UniRule annotation
    Gene namesi
    Name:ileS2UniRule annotation
    Ordered Locus Names:OB0362
    OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
    Taxonomic identifieri221109 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
    ProteomesiUP000000822: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10221022Isoleucine--tRNA ligase 2PRO_0000098552Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi221109.OB0362.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ET99.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 5011"HIGH" regionAdd
    BLAST
    Motifi582 – 5865"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ET99-1 [UniParc]FASTAAdd to Basket

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    MDHAQRERSM RAHWKENRTF QRSIENREGQ QTYVFYEGPP TANGMPHAGH     50
    ALGRTIKDFV ARYKTMSGYQ VLRKAGWDTH GLPVELEVEK QLNIRGKDDI 100
    EKFGVERFIE KCKESVFVYE NEWKEFTDKL GYWVDMEDPY KTLNNPYIES 150
    VWNILATIHD EDLLYKGHRV VPYCPNCETS LSSHEVAQGY KDVTDLSVTA 200
    KFRIKNKENE YILGWTTTPW TLPGNVALAV NPEMTYVKAK QGEEIYVVAK 250
    SLANTVLGEA YEVVEEVSGE DLVGINYEAP FDFISLDNGH YVIGADFVTD 300
    TSGTGIVHLN PAHGEDDYNA IQAKGMDFVN VVDSKGRYED VIAPLAGQFV 350
    KDSDVDIIKM LAKDGTLYEK QKYEHSYPHC WRCDTPLLYY AMEGWFIKTT 400
    AVKERMQENN QSVEWFPNHM RDGRFGNFLD NMVDWNIGRN RYWGTPLNVW 450
    VCDDCDKQKS PHSIQELRAF ADSDVPEDIE LHKPYVDKIT FDCECGGKMH 500
    RTKEVIDVWF DSGSMPFAQY HHPFENDELF NKQFPADVVI EGVDQTRGWF 550
    YSLLAVSTLF TGKTPYKRVL SLGHILDEHG RKMSKSKGNA LSPVELIEEF 600
    GADALRWALL ADSSPWNNKR FSKKTVSQAK SKVIDTILNV YSFYSMYADI 650
    DKFNVAEHAT DAKTTLDSWI LSRLNSVIKE VTTSLDQYDI TKGARSIALF 700
    VDELSNWYVR RSRQRFWSSG MNEDKKAAFT TLHEVLSKLT QLMAPYIPFV 750
    ADDIYTNLQG DSVHVSDFPK VDEAAIDEKL EADMQGVLEV IEGARSLRNE 800
    VNIKTKQPLA ELVVVPNDEQ LQSGLQAYTS IIEEEINVKQ VSFKKDTADF 850
    LDTEMKLNFP VAGPKLGKQV GQAKGKVENF TEQEKEAFLV NGTINVELQD 900
    GSEVTLEKED IIVEKKGQEG YQLFEGNRFV LLLDTNLTEK LKEEGFVREF 950
    IRAVQTYRKE LDLPVEQRVD IYVNSDENAE RILSEFDALI HDGLIINSVQ 1000
    FAEKEDMKQV TVNDTEMGLS IV 1022
    Length:1,022
    Mass (Da):116,800
    Last modified:March 1, 2003 - v1
    Checksum:i4BCF597F1774244B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC12318.1.
    RefSeqiNP_691283.1. NC_004193.1.

    Genome annotation databases

    EnsemblBacteriaiBAC12318; BAC12318; BAC12318.
    GeneIDi1015648.
    KEGGioih:OB0362.
    PATRICi22791639. VBIOceIhe82024_0373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC12318.1 .
    RefSeqi NP_691283.1. NC_004193.1.

    3D structure databases

    ProteinModelPortali Q8ET99.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221109.OB0362.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC12318 ; BAC12318 ; BAC12318 .
    GeneIDi 1015648.
    KEGGi oih:OB0362.
    PATRICi 22791639. VBIOceIhe82024_0373.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci OIHE221109:GI2A-408-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
      Takami H., Takaki Y., Uchiyama I.
      Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

    Entry informationi

    Entry nameiSYI2_OCEIH
    AccessioniPrimary (citable) accession number: Q8ET99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3