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Protein

Hydroxyethylthiazole kinase 2

Gene

thiM2

Organism
Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase 2 (thiM2), Hydroxyethylthiazole kinase 1 (thiM1)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40Substrate; via amide nitrogenUniRule annotation1
Binding sitei116ATPUniRule annotation1
Binding sitei161ATPUniRule annotation1
Binding sitei188Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinase 2UniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase 2UniRule annotation
Short name:
TH kinase 2UniRule annotation
Short name:
Thz kinase 2UniRule annotation
Gene namesi
Name:thiM2UniRule annotation
Ordered Locus Names:OB0643
OrganismiOceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
Proteomesi
  • UP000000822 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001569471 – 260Hydroxyethylthiazole kinase 2Add BLAST260

Interactioni

Protein-protein interaction databases

STRINGi221109.OB0643.

Structurei

3D structure databases

ProteinModelPortaliQ8ESJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
HOGENOMiHOG000114352.
KOiK00878.
OMAiANIMLAC.
OrthoDBiPOG091H004S.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8ESJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDAIAKIKQ DKPFIFNITN EVASNFSANG LIAIGASPAM SHTPREAKKY
60 70 80 90 100
GEIADAVVLN LGTLTEDRGE AMLKAGMAAN KNGVPVILDP IAVGGTDFRT
110 120 130 140 150
EIIDDILKTV KLAAIRANAG EIAVLAGKLE KAKGPDSIIQ ENEPEIAKTV
160 170 180 190 200
AKKYDTVVIS TGKVDVVTDG KRTALCTNGH EMLQNITASG CLLSSFVGPF
210 220 230 240 250
VSVVDDFYQA GIYAVTSYGI AAELAMEKAS GPGTFIPALL DEIYFLTDEK
260
VEKYKQVHEL
Length:260
Mass (Da):27,636
Last modified:March 1, 2003 - v1
Checksum:i7E86AF3F3A4B3785
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12599.1.
RefSeqiWP_011065049.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC12599; BAC12599; BAC12599.
KEGGioih:OB0643.
PATRICi22792237. VBIOceIhe82024_0657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12599.1.
RefSeqiWP_011065049.1. NC_004193.1.

3D structure databases

ProteinModelPortaliQ8ESJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221109.OB0643.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC12599; BAC12599; BAC12599.
KEGGioih:OB0643.
PATRICi22792237. VBIOceIhe82024_0657.

Phylogenomic databases

eggNOGiENOG4105C4U. Bacteria.
COG2145. LUCA.
HOGENOMiHOG000114352.
KOiK00878.
OMAiANIMLAC.
OrthoDBiPOG091H004S.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
InterProiIPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF02110. HK. 1 hit.
[Graphical view]
PIRSFiPIRSF000513. Thz_kinase. 1 hit.
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIM2_OCEIH
AccessioniPrimary (citable) accession number: Q8ESJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.