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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei195 – 1951ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-1282-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:OB1208
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Tryptophan--tRNA ligasePRO_0000136655Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB1208.

Structurei

3D structure databases

ProteinModelPortaliQ8ERU2.
SMRiQ8ERU2. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 189"HIGH" region
Motifi192 – 1965"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ERU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTIFSGIQP SGTLTLGNYL GAIQQFVELQ NDYNCYFCIV DEHAITVPQD
60 70 80 90 100
RLELRKNIKS LAALYIASGI DPDSSTLFIQ SEVPEHTQLG WMLQSISYVG
110 120 130 140 150
ELERMTQYKD KSQGQEAISS ALLTYPSLMA ADILLYNTDV VPVGDDQKQH
160 170 180 190 200
LELARNLAQR FNNRFNDIFT VPEVRIPKVG ARIMSLQEPT KKMSKSDTNQ
210 220 230 240 250
KGFISMLDEP KRIEKKIKSA VTDSEGIVKF DKENKPGVSN LLTIYSSCTG
260 270 280 290 300
ESIADLEKKY DGKGYGDFKQ GVANAVIDTL RPIQEKYEQL IQSDELDAIL
310 320
DQGRDKASFS AGKTIKKAKK AMGLGRK
Length:327
Mass (Da):36,494
Last modified:March 1, 2003 - v1
Checksum:i041EEC15D2CB9DEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC13164.1.
RefSeqiNP_692129.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC13164; BAC13164; BAC13164.
KEGGioih:OB1208.
PATRICi22793418. VBIOceIhe82024_1226.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC13164.1.
RefSeqiNP_692129.1. NC_004193.1.

3D structure databases

ProteinModelPortaliQ8ERU2.
SMRiQ8ERU2. Positions 1-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221109.OB1208.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC13164; BAC13164; BAC13164.
KEGGioih:OB1208.
PATRICi22793418. VBIOceIhe82024_1226.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059940.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG686NJQ.

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-1282-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiSYW_OCEIH
AccessioniPrimary (citable) accession number: Q8ERU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.