Reviewed, UniProtKB/Swiss-Prot Q8ERJ2 (GUAC_OCEIH)

Names and origin

Protein names

Recommended name:
    GMP reductase
    EC=1.7.1.7
Alternative name(s):
    Guanosine 5'-monophosphate oxidoreductase
      Short name=Guanosine monophosphate reductase

Gene names

Name:	guaC
Ordered Locus Names:	OB1310

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides By similarity. HAMAP MF_01511
Catalytic activity	Inosine 5'-phosphate + NH3 + NADP+ = guanosine 5'-phosphate + NADPH. HAMAP MF_01511
Sequence similarities	Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine nucleotide metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	GMP reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	GMP reductase HAMAP MF_01511		PRO_0000093762
Regions
	Nucleotide binding	204 – 227	24	NADP By similarity
Sites
	Active site	175	1	Thioimidate intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ERJ2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F10D02B8A546BAEE	FASTA	327	36,203
	10 20 30 40 50 60 MENVFDYEDI QLIPAKCVVN SRSECDTSVT LGNRTFKLPV VPANMQTIID EKIAKYLAEK 70 80 90 100 110 120 NYFYIMHRFE PEKRIDFIQD MQEYNLFTSI SVGVKEEEYT FIEDLAAKQL IPDYITIDIA 130 140 150 160 170 180 HGHSDAVIKM IKHIKNNLPS SFVIAGNVGT PEAVRELENA GADATKVGIG PGKVCITKIK 190 200 210 220 230 240 TGFGTGGWQL AALRWCAKAA SKPIIADGGI RTHGDIAKSI RFGASMVMIG SLFAGHEESP 250 260 270 280 290 300 GETIEQDGKK IKEYFGSASE FQKGEKKNVE GKKMYVEHKG PLQDTLTEME QDLQSSISYA 310 320 GGKQLDAIRN VDYVVVKNSI FNGDKVF

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC13266.1.
RefSeq	NP_692231.1.
3D structure databases
SMR	Q8ERJ2. Positions 1-321.
ModBase	Search...
Genome annotation databases
GeneID	1017488.
GenomeReviews	Gene locus OB1310 in contig BA000028_GR.
KEGG	oih:OB1310.
NMPDR	fig|221109.1.peg.1314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG298985.
OMA	FAGHEES.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1310-MONOMER.
BRENDA	1.7.1.7. 278212.
Family and domain databases
HAMAP	MF_01511. GMP_reduct_type2. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005994. GMP_reduct2. IPR015875. IMP_DH/GMP_Rdtase_CS. IPR001093. IMP_DH_GMPRt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00478. IMPDH. 1 hit. [Graphical view]
PIRSF	PIRSF036500. GMP_red_Firmic. 1 hit.
TIGRFAMs	TIGR01306. GMP_reduct_2. 1 hit.
PROSITE	PS00487. IMP_DH_GMP_RED. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAC_OCEIH

Accession

Primary (citable) accession number: Q8ERJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2004
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)