Reviewed, UniProtKB/Swiss-Prot Q8ERF4 (ROCA_OCEIH)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	OB1349

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056513
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ERF4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 14B272164951F531	FASTA	515	57,295
	10 20 30 40 50 60 MVLPFKHEPF TDFTVKENRK DYQDALAKVK EELGKDYPLV INGEKIYTDD KLISINPSNK 70 80 90 100 110 120 NEVVGNVSKA TKQHIEEAFT SAKEAFKEWK SWSAEDRARV LYRAAAIVRR RKHEFSAWLS 130 140 150 160 170 180 YDAGKPWDQA DGDTAEGIDF LEYYARHMVE LEKGKPLADR PNEDNKYFYQ PLGPGVVIPP 190 200 210 220 230 240 WNFAFAIVCG TTVAPIVAGN PVLLKPSENT PVIAYKLVEV LEEAGLPKGV LNFVPGDPAE 250 260 270 280 290 300 IGDYLVDHKD THFINFTGSR ATGVRIFERA TKIQDGQTHL KRIVAEMGGK DTIIVDESAD 310 320 330 340 350 360 LDLAAESIVH SAFGFSGQKC SACSRAVIHE SVYDEVIEKS VELAKTLTVG NPTEDNVYMA 370 380 390 400 410 420 SVVNQKQFDK IKDYIEVGKQ EGELVFGGET DDNKGFFVHP TIFKDLDPKA RIMQEEIFGP 430 440 450 460 470 480 VVAFSKAKSF DELLDIANNT EYGLTGAVIS NNRENLNRAQ TEFLVGNLYF NRGCTAAIVG 490 500 510 YQPFGGFKMS GTDSKAGGPD YLQHFLNAKV VTERF

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC13305.1.
RefSeq	NP_692270.1.
3D structure databases
SMR	Q8ERF4. Positions 2-515.
ModBase	Search...
Genome annotation databases
GeneID	1017529.
GenomeReviews	Gene locus OB1349 in contig BA000028_GR.
KEGG	oih:OB1349.
NMPDR	fig|221109.1.peg.1355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	TPYKHEP.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1349-MONOMER.
BRENDA	1.5.1.12. 278212.
Family and domain databases
HAMAP	MF_00733. RocA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_OCEIH

Accession

Primary (citable) accession number: Q8ERF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)