Reviewed, UniProtKB/Swiss-Prot Q8ER41 (SYI1_OCEIH)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase 1
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase 1
      Short name=IleRS 1

Gene names

Name:	ileS1
Ordered Locus Names:	OB1484

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Isoleucyl-tRNA synthetase 1 HAMAP MF_02002		PRO_0000098432
Regions
	Motif	57 – 67	11	"HIGH" region HAMAP MF_02002
	Motif	594 – 598	5	"KMSKS" region HAMAP MF_02002
Sites
	Metal binding	885	1	Zinc By similarity
	Metal binding	888	1	Zinc By similarity
	Metal binding	905	1	Zinc By similarity
	Metal binding	908	1	Zinc By similarity
	Binding site	553	1	Aminoacyl-adenylate By similarity
	Binding site	597	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ER41-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CDCAD9A3F063C7B7	FASTA	918	104,887
	10 20 30 40 50 60 MDYKQTLLMP KTAFPMRGNL PNKEPERQKK WEETNQYAKT LERTKGRPLF VLHDGPPYAN 70 80 90 100 110 120 GDIHIGHALN KVLKDFIVRY KSMTGYHAPY IPGWDTHGLP IETALTKKKK IKRKEMDIAA 130 140 150 160 170 180 FRKLCEEYAL GQINNQREQF KQLGVRGDWD NPYITLTKDY EASQIKVFGD MARKGYIYKG 190 200 210 220 230 240 LKPVYWSPSS ESALAEAEIE YQDKRSPSIY VAFEVKGGQA LLSGGEKFII WTTTPWTLPA 250 260 270 280 290 300 NLGISLHADL TYIVVQVGEE KYIIAEALFD DVSESLGWEN PQVLQSFKGK EAEGIEAQHP 310 320 330 340 350 360 FYDRTSLVML GEHVTTDAGT GCVHTAPGHG EDDFYVSRSY GIDAFCPVDE KGVFTQEAPG 370 380 390 400 410 420 FEGLFYDEAN KIITEKLDAS GALLKLEFIT HSYPHDWRTK KPTIFRATSQ WFASIKDFRV 430 440 450 460 470 480 DILEEIKQVN WYPHWGETRL YNMVRDREDW CISRQRAWGV PIPVFYGEDG TPIITDETIN 490 500 510 520 530 540 HVSELFREHG SNIWFEKEAK ELLPEGFTSE HSPNGNFAKE TDIMDVWFDS GSSHEGVLLN 550 560 570 580 590 600 RQNHQRPANV YLEGSDQYRG WFNSSLSTSV AVTGKAPYKA VISHGFVLDG NGRKMSKSLG 610 620 630 640 650 660 NVIVPSKVQK QLGSDILRLW VSSVDYQADV RISDDILKQT SESYRKIRNT FRFLLANLAD 670 680 690 700 710 720 FNPNTDRVKE ENMEEVDRYM VHRLQNVLAE AHKNYNQYEF APVFQQIHHF CSVDLSSFYL 730 740 750 760 770 780 DFAKDILYIE AKDHPRRRSI QTGYYEVLTS LVKLIAPIIP HTAEEVWEYI PEPEAESVHL 790 800 810 820 830 840 TDIPEARDVA INEQTVDKWN HFMKIRDDVL KALEEARSEK VIGKSLEAKI SIAAKDEGTK 850 860 870 880 890 900 KVLDEMEHLH QYFIVSEAVI VDTLTDAKEG NVVNVQVEVH PGETCDRCWV SSETVGENKN 910 HPSLCSRCAD VVTKHYAD

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000028 Genomic DNA. Translation: BAC13440.1.
RefSeq	NP_692405.1.
3D structure databases
HSSP	HSSP built from PDB template 1QU3 based on UniProtKB P41972.
ModBase	Search...
Genome annotation databases
GeneID	1017739.
GenomeReviews	Gene locus OB1484 in contig BA000028_GR.
KEGG	oih:OB1484.
NMPDR	fig|221109.1.peg.1486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ER41.
OMA	FPMRGNL.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1484-MON.
BRENDA	6.1.1.5. 278212.
Family and domain databases
HAMAP	MF_02002. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR010663. DNA_glyclase/IsotRNA_synth_Znf. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. PF06827. zf-FPG_IleRS. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI1_OCEIH

Accession

Primary (citable) accession number: Q8ER41

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)