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Q8ER41

- SYI1_OCEIH

UniProt

Q8ER41 - SYI1_OCEIH

Protein

Isoleucine--tRNA ligase 1

Gene

ileS1

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei553 – 5531Aminoacyl-adenylateUniRule annotation
    Binding sitei597 – 5971ATPUniRule annotation
    Metal bindingi885 – 8851ZincUniRule annotation
    Metal bindingi888 – 8881ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciOIHE221109:GI2A-1562-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligase 1UniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetase 1UniRule annotation
    Short name:
    IleRS 1UniRule annotation
    Gene namesi
    Name:ileS1UniRule annotation
    Ordered Locus Names:OB1484
    OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
    Taxonomic identifieri221109 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
    ProteomesiUP000000822: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 918918Isoleucine--tRNA ligase 1PRO_0000098432Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi221109.OB1484.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8ER41.
    SMRiQ8ER41. Positions 2-866.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi594 – 5985"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiLGHRVHY.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8ER41-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKQTLLMP KTAFPMRGNL PNKEPERQKK WEETNQYAKT LERTKGRPLF    50
    VLHDGPPYAN GDIHIGHALN KVLKDFIVRY KSMTGYHAPY IPGWDTHGLP 100
    IETALTKKKK IKRKEMDIAA FRKLCEEYAL GQINNQREQF KQLGVRGDWD 150
    NPYITLTKDY EASQIKVFGD MARKGYIYKG LKPVYWSPSS ESALAEAEIE 200
    YQDKRSPSIY VAFEVKGGQA LLSGGEKFII WTTTPWTLPA NLGISLHADL 250
    TYIVVQVGEE KYIIAEALFD DVSESLGWEN PQVLQSFKGK EAEGIEAQHP 300
    FYDRTSLVML GEHVTTDAGT GCVHTAPGHG EDDFYVSRSY GIDAFCPVDE 350
    KGVFTQEAPG FEGLFYDEAN KIITEKLDAS GALLKLEFIT HSYPHDWRTK 400
    KPTIFRATSQ WFASIKDFRV DILEEIKQVN WYPHWGETRL YNMVRDREDW 450
    CISRQRAWGV PIPVFYGEDG TPIITDETIN HVSELFREHG SNIWFEKEAK 500
    ELLPEGFTSE HSPNGNFAKE TDIMDVWFDS GSSHEGVLLN RQNHQRPANV 550
    YLEGSDQYRG WFNSSLSTSV AVTGKAPYKA VISHGFVLDG NGRKMSKSLG 600
    NVIVPSKVQK QLGSDILRLW VSSVDYQADV RISDDILKQT SESYRKIRNT 650
    FRFLLANLAD FNPNTDRVKE ENMEEVDRYM VHRLQNVLAE AHKNYNQYEF 700
    APVFQQIHHF CSVDLSSFYL DFAKDILYIE AKDHPRRRSI QTGYYEVLTS 750
    LVKLIAPIIP HTAEEVWEYI PEPEAESVHL TDIPEARDVA INEQTVDKWN 800
    HFMKIRDDVL KALEEARSEK VIGKSLEAKI SIAAKDEGTK KVLDEMEHLH 850
    QYFIVSEAVI VDTLTDAKEG NVVNVQVEVH PGETCDRCWV SSETVGENKN 900
    HPSLCSRCAD VVTKHYAD 918
    Length:918
    Mass (Da):104,887
    Last modified:March 1, 2003 - v1
    Checksum:iCDCAD9A3F063C7B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC13440.1.
    RefSeqiNP_692405.1. NC_004193.1.
    WP_011065885.1. NC_004193.1.

    Genome annotation databases

    EnsemblBacteriaiBAC13440; BAC13440; BAC13440.
    GeneIDi1017739.
    KEGGioih:OB1484.
    PATRICi22793988. VBIOceIhe82024_1510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC13440.1 .
    RefSeqi NP_692405.1. NC_004193.1.
    WP_011065885.1. NC_004193.1.

    3D structure databases

    ProteinModelPortali Q8ER41.
    SMRi Q8ER41. Positions 2-866.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221109.OB1484.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC13440 ; BAC13440 ; BAC13440 .
    GeneIDi 1017739.
    KEGGi oih:OB1484.
    PATRICi 22793988. VBIOceIhe82024_1510.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi LGHRVHY.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci OIHE221109:GI2A-1562-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
      Takami H., Takaki Y., Uchiyama I.
      Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

    Entry informationi

    Entry nameiSYI1_OCEIH
    AccessioniPrimary (citable) accession number: Q8ER41
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3