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Q8ER41

- SYI1_OCEIH

UniProt

Q8ER41 - SYI1_OCEIH

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Protein

Isoleucine--tRNA ligase 1

Gene
ileS1, OB1484
Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi885 – 8851Zinc By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-1562-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 1 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 1
Short name:
IleRS 1
Gene namesi
Name:ileS1
Ordered Locus Names:OB1484
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligase 1UniRule annotationPRO_0000098432Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB1484.

Structurei

3D structure databases

ProteinModelPortaliQ8ER41.
SMRiQ8ER41. Positions 2-866.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8ER41-1 [UniParc]FASTAAdd to Basket

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MDYKQTLLMP KTAFPMRGNL PNKEPERQKK WEETNQYAKT LERTKGRPLF    50
VLHDGPPYAN GDIHIGHALN KVLKDFIVRY KSMTGYHAPY IPGWDTHGLP 100
IETALTKKKK IKRKEMDIAA FRKLCEEYAL GQINNQREQF KQLGVRGDWD 150
NPYITLTKDY EASQIKVFGD MARKGYIYKG LKPVYWSPSS ESALAEAEIE 200
YQDKRSPSIY VAFEVKGGQA LLSGGEKFII WTTTPWTLPA NLGISLHADL 250
TYIVVQVGEE KYIIAEALFD DVSESLGWEN PQVLQSFKGK EAEGIEAQHP 300
FYDRTSLVML GEHVTTDAGT GCVHTAPGHG EDDFYVSRSY GIDAFCPVDE 350
KGVFTQEAPG FEGLFYDEAN KIITEKLDAS GALLKLEFIT HSYPHDWRTK 400
KPTIFRATSQ WFASIKDFRV DILEEIKQVN WYPHWGETRL YNMVRDREDW 450
CISRQRAWGV PIPVFYGEDG TPIITDETIN HVSELFREHG SNIWFEKEAK 500
ELLPEGFTSE HSPNGNFAKE TDIMDVWFDS GSSHEGVLLN RQNHQRPANV 550
YLEGSDQYRG WFNSSLSTSV AVTGKAPYKA VISHGFVLDG NGRKMSKSLG 600
NVIVPSKVQK QLGSDILRLW VSSVDYQADV RISDDILKQT SESYRKIRNT 650
FRFLLANLAD FNPNTDRVKE ENMEEVDRYM VHRLQNVLAE AHKNYNQYEF 700
APVFQQIHHF CSVDLSSFYL DFAKDILYIE AKDHPRRRSI QTGYYEVLTS 750
LVKLIAPIIP HTAEEVWEYI PEPEAESVHL TDIPEARDVA INEQTVDKWN 800
HFMKIRDDVL KALEEARSEK VIGKSLEAKI SIAAKDEGTK KVLDEMEHLH 850
QYFIVSEAVI VDTLTDAKEG NVVNVQVEVH PGETCDRCWV SSETVGENKN 900
HPSLCSRCAD VVTKHYAD 918
Length:918
Mass (Da):104,887
Last modified:March 1, 2003 - v1
Checksum:iCDCAD9A3F063C7B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC13440.1.
RefSeqiNP_692405.1. NC_004193.1.
WP_011065885.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC13440; BAC13440; BAC13440.
GeneIDi1017739.
KEGGioih:OB1484.
PATRICi22793988. VBIOceIhe82024_1510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC13440.1 .
RefSeqi NP_692405.1. NC_004193.1.
WP_011065885.1. NC_004193.1.

3D structure databases

ProteinModelPortali Q8ER41.
SMRi Q8ER41. Positions 2-866.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221109.OB1484.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC13440 ; BAC13440 ; BAC13440 .
GeneIDi 1017739.
KEGGi oih:OB1484.
PATRICi 22793988. VBIOceIhe82024_1510.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci OIHE221109:GI2A-1562-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiSYI1_OCEIH
AccessioniPrimary (citable) accession number: Q8ER41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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