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Protein

Isoleucine--tRNA ligase 1

Gene

ileS1

Organism
Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei553Aminoacyl-adenylateUniRule annotation1
Binding sitei597ATPUniRule annotation1
Metal bindingi885ZincUniRule annotation1
Metal bindingi888ZincUniRule annotation1
Metal bindingi905ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciOIHE221109:G1G3F-1570-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 1UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 1UniRule annotation
Short name:
IleRS 1UniRule annotation
Gene namesi
Name:ileS1UniRule annotation
Ordered Locus Names:OB1484
OrganismiOceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
Proteomesi
  • UP000000822 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984321 – 918Isoleucine--tRNA ligase 1Add BLAST918

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB1484

Structurei

3D structure databases

ProteinModelPortaliQ8ER41
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi594 – 598"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ER41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKQTLLMP KTAFPMRGNL PNKEPERQKK WEETNQYAKT LERTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHIGHALN KVLKDFIVRY KSMTGYHAPY IPGWDTHGLP
110 120 130 140 150
IETALTKKKK IKRKEMDIAA FRKLCEEYAL GQINNQREQF KQLGVRGDWD
160 170 180 190 200
NPYITLTKDY EASQIKVFGD MARKGYIYKG LKPVYWSPSS ESALAEAEIE
210 220 230 240 250
YQDKRSPSIY VAFEVKGGQA LLSGGEKFII WTTTPWTLPA NLGISLHADL
260 270 280 290 300
TYIVVQVGEE KYIIAEALFD DVSESLGWEN PQVLQSFKGK EAEGIEAQHP
310 320 330 340 350
FYDRTSLVML GEHVTTDAGT GCVHTAPGHG EDDFYVSRSY GIDAFCPVDE
360 370 380 390 400
KGVFTQEAPG FEGLFYDEAN KIITEKLDAS GALLKLEFIT HSYPHDWRTK
410 420 430 440 450
KPTIFRATSQ WFASIKDFRV DILEEIKQVN WYPHWGETRL YNMVRDREDW
460 470 480 490 500
CISRQRAWGV PIPVFYGEDG TPIITDETIN HVSELFREHG SNIWFEKEAK
510 520 530 540 550
ELLPEGFTSE HSPNGNFAKE TDIMDVWFDS GSSHEGVLLN RQNHQRPANV
560 570 580 590 600
YLEGSDQYRG WFNSSLSTSV AVTGKAPYKA VISHGFVLDG NGRKMSKSLG
610 620 630 640 650
NVIVPSKVQK QLGSDILRLW VSSVDYQADV RISDDILKQT SESYRKIRNT
660 670 680 690 700
FRFLLANLAD FNPNTDRVKE ENMEEVDRYM VHRLQNVLAE AHKNYNQYEF
710 720 730 740 750
APVFQQIHHF CSVDLSSFYL DFAKDILYIE AKDHPRRRSI QTGYYEVLTS
760 770 780 790 800
LVKLIAPIIP HTAEEVWEYI PEPEAESVHL TDIPEARDVA INEQTVDKWN
810 820 830 840 850
HFMKIRDDVL KALEEARSEK VIGKSLEAKI SIAAKDEGTK KVLDEMEHLH
860 870 880 890 900
QYFIVSEAVI VDTLTDAKEG NVVNVQVEVH PGETCDRCWV SSETVGENKN
910
HPSLCSRCAD VVTKHYAD
Length:918
Mass (Da):104,887
Last modified:March 1, 2003 - v1
Checksum:iCDCAD9A3F063C7B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA Translation: BAC13440.1
RefSeqiWP_011065885.1, NC_004193.1

Genome annotation databases

EnsemblBacteriaiBAC13440; BAC13440; BAC13440
KEGGioih:OB1484

Similar proteinsi

Entry informationi

Entry nameiSYI1_OCEIH
AccessioniPrimary (citable) accession number: Q8ER41
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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