Reviewed, UniProtKB/Swiss-Prot Q8EQP2 (CYSH_OCEIH)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	OB1652

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008931

Sequences

	Sequence		Length	Mass (Da)
	Q8EQP2-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 083D781AA9190CD9	FASTA	240	27,407
	10 20 30 40 50 60 MGGYSVTYSN FVKDPYKDWF PDDETNGASK ILQWAYEVYG TDIVYACSFG AEGMVLIDLI 70 80 90 100 110 120 SKTQKNADIV FLDTDLHFQE TYDLIEDIKV KYPTLNIHIK KPDLTLEEQA AEHGSALWKR 130 140 150 160 170 180 QPDQCCYIRK IKPLEDALSG APAWISGLRR EQSVSRSKTD FINKDERFKS IKVCPLIHWT 190 200 210 220 230 240 WDDVWEYIKD NNLPYNELHD NGYPSIGCIP CTSQVSDSSD SRAGRWAGTQ KTECGLHTSD

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000028 Genomic DNA. Translation: BAC13608.1.
RefSeq	NP_692573.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1018148.
GenomeReviews	Gene locus OB1652 in contig BA000028_GR.
KEGG	oih:OB1652.
NMPDR	fig|221109.1.peg.1655.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8EQP2.
OMA	FEETLAY.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1652-MON.
BRENDA	1.8.4.8. 278212.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_OCEIH

Accession

Primary (citable) accession number: Q8EQP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)