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Q8EQJ1 (DAPA_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:OB1708
OrganismOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]
Taxonomic identifier221109 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 295295Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000103132

Regions

Region49 – 502Pyruvate binding By similarity

Sites

Active site1621Schiff-base intermediate with substrate By similarity
Binding site1071Pyruvate By similarity
Site1341Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EQJ1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: BCC963F5E154ED2A

FASTA29532,253
        10         20         30         40         50         60 
MNFGKVLTAM VTPFDKHEEI DFQALDHLID HLINNGSDGL VVAGTTGESP TLTHEERIKL 

        70         80         90        100        110        120 
FEYVVKKVNG AVPVIAGTGS NYTKASIELT KEVAKTGVDG VMLVAPYYNK PSQDSMYEHF 

       130        140        150        160        170        180 
KVIAESTELP VMLYNVPGRT GVDMDVSTVV RLSQIDNIVS IKDASGNLEK MTNIIRLTDD 

       190        200        210        220        230        240 
NFSVYSGEDS LTLPALAIGA DGIVSVSSHI IGNDMQEMIQ RFEQGQVAEA ANIHQRVLPV 

       250        260        270        280        290 
MQEMFANPSP VPVKTALNLQ SVPVGKVRLP LIELTDEQLQ NLQSVLSDFE HSKMI

« Hide

References

[1]"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
Takami H., Takaki Y., Uchiyama I.
Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000028 Genomic DNA. Translation: BAC13664.1.
RefSeqNP_692629.1. NC_004193.1.

3D structure databases

ProteinModelPortalQ8EQJ1.
SMRQ8EQJ1. Positions 1-286.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1018204.
GenomeReviewsGene locus OB1708 in contig BA000028_GR.
KEGGoih:OB1708.
NMPDRfig|221109.1.peg.1711.
PATRIC22794442. VBIOceIhe82024_1737.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMAFMLCGGH.
PhylomeDBQ8EQJ1.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycOIHE221109:OB1708-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_OCEIH
AccessionPrimary (citable) accession number: Q8EQJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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