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Q8EP12

- SYL_OCEIH

UniProt

Q8EP12 - SYL_OCEIH

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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei579 – 5791ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-2390-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:OB2307
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Leucine--tRNA ligasePRO_0000152057Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi221109.OB2307.

Structurei

3D structure databases

ProteinModelPortaliQ8EP12.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi576 – 5805"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiHISHELW.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EP12-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSFQHRQIEK KWQDYWEDNK TFKTDTFSNK EKFYALDMFP YPSGQGLHVG
60 70 80 90 100
HPEGYTATDI LARMKRMQGY EVMHPMGWDA FGLPAEQYAI DTGNSPAEFT
110 120 130 140 150
NQNINTFKRQ IKELGFSYDW DREINTTDPN YYKWTQWIFK KLYENDLAYM
160 170 180 190 200
DEVAVNWCPA LGTVLANEEV IDGKSERGGH PVIRKPMKQW MLKITAYADR
210 220 230 240 250
LLEDLNELDW PDSLKEMQRN WIGRSEGAEV TFSIKGNEGT FTVFTTRPDT
260 270 280 290 300
LFGATYAVVA PEHPLVKEIT TLSQKEAVQN YLDEIQTKSD LERTDLAKDK
310 320 330 340 350
TGVFTGAYAI NPVNGEEMPI WVADYVLMSY GTGAIMAVPA HDERDYEFAN
360 370 380 390 400
TFGLPIKEVV AGGNIDDEAY TGDGEHVNSE FLNGLGKEEA ITKMIDWLEA
410 420 430 440 450
NGSGEKKITY RLRDWLFARQ RYWGEPIPII HWEDGTMTAV PDEDLPLTLP
460 470 480 490 500
QVAEIKPSGT GESPLANNTD WVNVVDPETG LKGRRETNTM PQWAGSCWYF
510 520 530 540 550
LRYIDPNNTE RLADPKALEE WLPIDIYIGG AEHAVLHLLY ARFWHKFLFD
560 570 580 590 600
IGVVSTKEPF QKLYNQGMIL GEGNEKMSKS KGNVVNPDDI IDSHGADTLR
610 620 630 640 650
LYEMFMGPLD ASVAWSTNGL DGSRRFLDRV WRLYVGDDGS LTDKIIDDES
660 670 680 690 700
TELDKIYHET VKKVTDDFEH MHFNTGISQM MVFINECYKA NKIPKIYAEG
710 720 730 740 750
FVKLLSPVAP HLSEEIWQKL GHSESISKAT WPAYDESKLV EDEVEVVLQI
760 770 780 790 800
MGKVRSKIQV PVDISKDDLE KAALDDESMQ KWLEGKTIRK VIVVPGKLVN

IVAN
Length:804
Mass (Da):91,702
Last modified:March 1, 2003 - v1
Checksum:iF0FFC32DBC4513FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC14263.1.
RefSeqiNP_693228.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC14263; BAC14263; BAC14263.
GeneIDi1015873.
KEGGioih:OB2307.
PATRICi22795670. VBIOceIhe82024_2340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC14263.1 .
RefSeqi NP_693228.1. NC_004193.1.

3D structure databases

ProteinModelPortali Q8EP12.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221109.OB2307.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC14263 ; BAC14263 ; BAC14263 .
GeneIDi 1015873.
KEGGi oih:OB2307.
PATRICi 22795670. VBIOceIhe82024_2340.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200748.
KOi K01869.
OMAi HISHELW.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci OIHE221109:GI2A-2390-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiSYL_OCEIH
AccessioniPrimary (citable) accession number: Q8EP12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3