Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8EP12

- SYL_OCEIH

UniProt

Q8EP12 - SYL_OCEIH

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei579 – 5791ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciOIHE221109:GI2A-2390-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:OB2307
    OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
    Taxonomic identifieri221109 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
    ProteomesiUP000000822: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 804804Leucine--tRNA ligasePRO_0000152057Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi221109.OB2307.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8EP12.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi40 – 5112"HIGH" regionAdd
    BLAST
    Motifi576 – 5805"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200748.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8EP12-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFQHRQIEK KWQDYWEDNK TFKTDTFSNK EKFYALDMFP YPSGQGLHVG    50
    HPEGYTATDI LARMKRMQGY EVMHPMGWDA FGLPAEQYAI DTGNSPAEFT 100
    NQNINTFKRQ IKELGFSYDW DREINTTDPN YYKWTQWIFK KLYENDLAYM 150
    DEVAVNWCPA LGTVLANEEV IDGKSERGGH PVIRKPMKQW MLKITAYADR 200
    LLEDLNELDW PDSLKEMQRN WIGRSEGAEV TFSIKGNEGT FTVFTTRPDT 250
    LFGATYAVVA PEHPLVKEIT TLSQKEAVQN YLDEIQTKSD LERTDLAKDK 300
    TGVFTGAYAI NPVNGEEMPI WVADYVLMSY GTGAIMAVPA HDERDYEFAN 350
    TFGLPIKEVV AGGNIDDEAY TGDGEHVNSE FLNGLGKEEA ITKMIDWLEA 400
    NGSGEKKITY RLRDWLFARQ RYWGEPIPII HWEDGTMTAV PDEDLPLTLP 450
    QVAEIKPSGT GESPLANNTD WVNVVDPETG LKGRRETNTM PQWAGSCWYF 500
    LRYIDPNNTE RLADPKALEE WLPIDIYIGG AEHAVLHLLY ARFWHKFLFD 550
    IGVVSTKEPF QKLYNQGMIL GEGNEKMSKS KGNVVNPDDI IDSHGADTLR 600
    LYEMFMGPLD ASVAWSTNGL DGSRRFLDRV WRLYVGDDGS LTDKIIDDES 650
    TELDKIYHET VKKVTDDFEH MHFNTGISQM MVFINECYKA NKIPKIYAEG 700
    FVKLLSPVAP HLSEEIWQKL GHSESISKAT WPAYDESKLV EDEVEVVLQI 750
    MGKVRSKIQV PVDISKDDLE KAALDDESMQ KWLEGKTIRK VIVVPGKLVN 800
    IVAN 804
    Length:804
    Mass (Da):91,702
    Last modified:March 1, 2003 - v1
    Checksum:iF0FFC32DBC4513FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC14263.1.
    RefSeqiNP_693228.1. NC_004193.1.

    Genome annotation databases

    EnsemblBacteriaiBAC14263; BAC14263; BAC14263.
    GeneIDi1015873.
    KEGGioih:OB2307.
    PATRICi22795670. VBIOceIhe82024_2340.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000028 Genomic DNA. Translation: BAC14263.1 .
    RefSeqi NP_693228.1. NC_004193.1.

    3D structure databases

    ProteinModelPortali Q8EP12.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 221109.OB2307.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC14263 ; BAC14263 ; BAC14263 .
    GeneIDi 1015873.
    KEGGi oih:OB2307.
    PATRICi 22795670. VBIOceIhe82024_2340.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200748.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci OIHE221109:GI2A-2390-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
      Takami H., Takaki Y., Uchiyama I.
      Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

    Entry informationi

    Entry nameiSYL_OCEIH
    AccessioniPrimary (citable) accession number: Q8EP12
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3