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Q8EP12 (SYL_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:OB2307
OrganismOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]
Taxonomic identifier221109 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152057

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EP12 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: F0FFC32DBC4513FC

FASTA80491,702
        10         20         30         40         50         60 
MSFQHRQIEK KWQDYWEDNK TFKTDTFSNK EKFYALDMFP YPSGQGLHVG HPEGYTATDI 

        70         80         90        100        110        120 
LARMKRMQGY EVMHPMGWDA FGLPAEQYAI DTGNSPAEFT NQNINTFKRQ IKELGFSYDW 

       130        140        150        160        170        180 
DREINTTDPN YYKWTQWIFK KLYENDLAYM DEVAVNWCPA LGTVLANEEV IDGKSERGGH 

       190        200        210        220        230        240 
PVIRKPMKQW MLKITAYADR LLEDLNELDW PDSLKEMQRN WIGRSEGAEV TFSIKGNEGT 

       250        260        270        280        290        300 
FTVFTTRPDT LFGATYAVVA PEHPLVKEIT TLSQKEAVQN YLDEIQTKSD LERTDLAKDK 

       310        320        330        340        350        360 
TGVFTGAYAI NPVNGEEMPI WVADYVLMSY GTGAIMAVPA HDERDYEFAN TFGLPIKEVV 

       370        380        390        400        410        420 
AGGNIDDEAY TGDGEHVNSE FLNGLGKEEA ITKMIDWLEA NGSGEKKITY RLRDWLFARQ 

       430        440        450        460        470        480 
RYWGEPIPII HWEDGTMTAV PDEDLPLTLP QVAEIKPSGT GESPLANNTD WVNVVDPETG 

       490        500        510        520        530        540 
LKGRRETNTM PQWAGSCWYF LRYIDPNNTE RLADPKALEE WLPIDIYIGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKFLFD IGVVSTKEPF QKLYNQGMIL GEGNEKMSKS KGNVVNPDDI IDSHGADTLR 

       610        620        630        640        650        660 
LYEMFMGPLD ASVAWSTNGL DGSRRFLDRV WRLYVGDDGS LTDKIIDDES TELDKIYHET 

       670        680        690        700        710        720 
VKKVTDDFEH MHFNTGISQM MVFINECYKA NKIPKIYAEG FVKLLSPVAP HLSEEIWQKL 

       730        740        750        760        770        780 
GHSESISKAT WPAYDESKLV EDEVEVVLQI MGKVRSKIQV PVDISKDDLE KAALDDESMQ 

       790        800 
KWLEGKTIRK VIVVPGKLVN IVAN 

« Hide

References

[1]"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
Takami H., Takaki Y., Uchiyama I.
Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000028 Genomic DNA. Translation: BAC14263.1.
RefSeqNP_693228.1. NC_004193.1.

3D structure databases

ProteinModelPortalQ8EP12.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221109.OB2307.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC14263; BAC14263; BAC14263.
GeneID1015873.
KEGGoih:OB2307.
PATRIC22795670. VBIOceIhe82024_2340.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycOIHE221109:GI2A-2390-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_OCEIH
AccessionPrimary (citable) accession number: Q8EP12
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries