Q8ENK7 (GLPK_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	OB2475

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_0000059473
Regions
	Nucleotide binding	411 – 415	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity
	Binding site	267	1	ATP By similarity
	Binding site	310	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ENK7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D48292EB263764FF	FASTA	500	55,668
	10 20 30 40 50 60 MSEKFILSID QGTTSSRAIL FNHKGEIVES AQKEFEQFFP KPGWVEHDAN EIWTSVLACI 70 80 90 100 110 120 ADVLRKADIE ANQIEGIGIT NQRETTVVWD KNTGRPIYKA IVWQSRQTEG ICKELKEQGH 130 140 150 160 170 180 EDTIRHKTGL LIDPYFSGTK VKWILDNVDG AREKAEAGEL SFGTIDSWLI YRLSGGKTHV 190 200 210 220 230 240 TDYSNASRTL MFNIYDLKWD DELLEILDVP KSMLPEVKPS SEVYANTVSY HFFGEEVPIA 250 260 270 280 290 300 GVAGDQQAAL FGQACFDQGM AKNTYGTGCF LLMNTGEEPV RSKHGLLTTI AWGVDGKVEY 310 320 330 340 350 360 ALEGSIFVAG SAIQWLRDGL RLIESSPQSE ALASQVETTD GVYLVPAFVG LGTPYWDSDA 370 380 390 400 410 420 RGAVFGLTRG TTKEHFVRAT LESLAYQTKD VMDAMLADSD IDLKKLRVDG GAVKNNLLMQ 430 440 450 460 470 480 FQSDILGVTV ERPVVQETTA LGSAYLAGLA VGFWKDKEEI AKQWLNEKTF ENEMSEEESS 490 500 YLYKGWQKAV EATRSFKFES

References

[1]	"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC14431.1.
RefSeq	NP_693396.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8ENK7.
SMR	Q8ENK7. Positions 5-490.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1015368.
GenomeReviews	Gene locus OB2475 in contig BA000028_GR.
KEGG	oih:OB2475.
NMPDR	fig|221109.1.peg.2474.
PATRIC	22796008. VBIOceIhe82024_2508.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	ALYGQLC.
PhylomeDB	Q8ENK7.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	OIHE221109:OB2475-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_OCEIH

Accession

Primary (citable) accession number: Q8ENK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program