Reviewed, UniProtKB/Swiss-Prot Q8EM86 (MURA1_OCEIH)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1
    EC=2.5.1.7
Alternative name(s):
    Enoylpyruvate transferase 1
    UDP-N-acetylglucosamine enolpyruvyl transferase 1
      Short name=EPT 1

Gene names

Name:	murA1
Synonyms:	murA
Ordered Locus Names:	OB2972

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Adds enolpyruvyl to UDP-N-acetylglucosamine By similarity. HAMAP MF_00111
Catalytic activity	Phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine. HAMAP MF_00111
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00111
Subcellular location	Cytoplasm Probable HAMAP MF_00111.
Sequence similarities	Belongs to the EPSP synthase family. MurA subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylglucosamine 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	UDP-N-acetylglucosamine 1-carboxyvinyltransferase 1 HAMAP MF_00111		PRO_0000231232
Sites
	Active site	119	1	Proton donor By similarity
	Binding site	119	1	Phosphoenolpyruvate (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EM86-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2C43ED4C173AE7F0	FASTA	443	47,651
	10 20 30 40 50 60 MEKIIVSGGH QLNGTVRLEG AKNAVLPVLA ASLIASEGES VIKEVPVLAD VYTINEVLRN 70 80 90 100 110 120 LNAEVEFDST TKTVNINASQ QLETEAPFEY VRKMRASVLV LGPLLARYGH AKVAMPGGCA 130 140 150 160 170 180 IGSRPIDLHL KGFEAMGAEI HVGNGYVEAN VNGRLQGAKI YLDMPSVGAT ENIMMAAALA 190 200 210 220 230 240 EGKTVIENAA KEPEIVDLAN YLNKMGANIV GAGTETIRII GVEKLRGTEH MIIPDRIEAG 250 260 270 280 290 300 TFMVASAITG GNVFIENAMR EHLRSVISKL EEMNVDVIDE NGGLRIIGPE KLKSTDIKTL 310 320 330 340 350 360 PHPGFPTDMQ SQMMSLMLRA EGTGVITETV FENRFMHVEE FRRMNANIKI EGRSVIIEGI 370 380 390 400 410 420 SELQGAEVAA TDLRAAAALI LAGLVSDGYT RVTELKHLDR GYVDIVDKLA ALGADIKRVD 430 440 ENGVVVQPLY VTAAKESNEI AAD

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC14928.1.
RefSeq	NP_693894.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1016743.
GenomeReviews	Gene locus OB2972 in contig BA000028_GR.
KEGG	oih:OB2972.
NMPDR	fig|221109.1.peg.2975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG482701.
OMA	RASNLVM.
Enzyme and pathway databases
BioCyc	OIHE221109:OB2972-MONOMER.
BRENDA	2.5.1.7. 278212.
Family and domain databases
HAMAP	MF_00111. MurA. [Tree]
InterPro	IPR001986. EPSP_synthase_core. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. IPR005750. UDP_GlcNAc_COvinyl_MurA. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
PANTHER	PTHR21090:SF4. AcGlu_Tran_MurA. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01072. murA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURA1_OCEIH

Accession

Primary (citable) accession number: Q8EM86

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)