Q8EM59 (KITH_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	OB2999

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP MF_00124
Subunit structure	Homotetramer By similarity. HAMAP MF_00124
Subcellular location	Cytoplasm Potential HAMAP MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Thymidine kinase HAMAP MF_00124		PRO_0000175000
Regions
	Nucleotide binding	15 – 22	8	ATP By similarity
	Nucleotide binding	88 – 91	4	ATP By similarity
Sites
	Active site	89	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	148	1	Zinc By similarity
	Metal binding	183	1	Zinc By similarity
	Metal binding	186	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EM59 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5F9666FE39018165	FASTA	207	23,268
	10 20 30 40 50 60 MYVMKQNGWV ELICGSMFSG KSEELIRRVR RATYAHINVR VYKPAIDDRY DDKAIVSHDG 70 80 90 100 110 120 TSTMARPIHD AVEILEDVDN NVEIVGIDEV QFFDNNVVDV IEELADRGIR VIVAGLDLDF 130 140 150 160 170 180 RGEPFEPVPK IMALAESVTK LNAICPICGS PASRTQRLID GKPASYNDPI ILVGASESYE 190 200 PRCRHHHEVP FRPENNFATK SWDALKS

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC14955.1.
RefSeq	NP_693921.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8EM59.
SMR	Q8EM59. Positions 1-190.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1016723.
GenomeReviews	Gene locus OB2999 in contig BA000028_GR.
KEGG	oih:OB2999.
NMPDR	fig|221109.1.peg.3001.
PATRIC	22797086. VBIOceIhe82024_3047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG522108.
OMA	EPRCRHH.
PhylomeDB	Q8EM59.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	OIHE221109:OB2999-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase. [Tree]
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
KO	K00857.
PANTHER	PTHR11441. TK_cell. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_OCEIH

Accession

Primary (citable) accession number: Q8EM59

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 14, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program