Reviewed, UniProtKB/Swiss-Prot Q8ELR9 (ALR1_OCEIH)

Names and origin

Protein names

Recommended name:
    Alanine racemase 1
    EC=5.1.1.1

Gene names

Name:	alr1
Ordered Locus Names:	OB3149

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alanine racemase 1 HAMAP MF_01201		PRO_0000114542
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	268	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ELR9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8EA1C02A19AFA3CF	FASTA	376	41,741
	10 20 30 40 50 60 MKVTSYRDAW VEVSLDALTY NVRQFKNHLQ EKSRLMAVVK ADGYGHGAVP IARRSLEAGA 70 80 90 100 110 120 EYLGVAFIDE ALQIREAGIL APILLLGFTP SYAVREAVRH DLTLTVYSTE TLEAIKDAAR 130 140 150 160 170 180 SLDKKAKIHI KVDSGMSRIG LRSSEEVISL MTSLQTEEIE VEGIFTHFAD ADNDESDAYT 190 200 210 220 230 240 YKQFETFQGI MEALENEGYS IPIKHCCNSA ATIAFPDMHM DMVRVGISLY GLYPGQHLKE 250 260 270 280 290 300 ILSLQQVMSF KAKPVLIKDV PPHQPISYGL TYETKETSKI ATLPIGYADG FSRLLSNVGH 310 320 330 340 350 360 VTVHGTACPI VGRICMDQSM IDVSEVQTDV TKDDIVTIFG DENAGYIPLD VVAEQMQTIH 370 YETVCLIGKR VPRHYV

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000028 Genomic DNA. Translation: BAC15105.1.
RefSeq	NP_694071.1.
3D structure databases
HSSP	HSSP built from PDB template 1BD0 based on UniProtKB P10724.
ModBase	Search...
Genome annotation databases
GeneID	1018093.
GenomeReviews	Gene locus OB3149 in contig BA000028_GR.
KEGG	oih:OB3149.
NMPDR	fig|221109.1.peg.3152.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8ELR9.
OMA	PTHERSI.
Enzyme and pathway databases
BioCyc	OIHE221109:OB3149-MON.
BRENDA	5.1.1.1. 278212.
Family and domain databases
HAMAP	MF_01201. [Tree]
InterPro	IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR000821. Ala_racemase_reg. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_OCEIH

Accession

Primary (citable) accession number: Q8ELR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 30, 2004
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)