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Q8EL95 (MT36_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative modification methylase OB3336

EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase
M.OihORF3336P
Gene names
Ordered Locus Names:OB3336
OrganismOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]
Taxonomic identifier221109 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus

Protein attributes

Sequence length460 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by a specific endonuclease Potential.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 460460Putative modification methylase OB3336
PRO_0000087915

Regions

Domain15 – 458444SAM-dependent MTase C5-type

Sites

Active site971 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EL95 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5483B86933CDBD0C

FASTA46052,508
        10         20         30         40         50         60 
MHQIYSATST DKKLPEVVDL FSGCGGLALG FQLAGFNIRK GIELDRDASD VASFNLHWRQ 

        70         80         90        100        110        120 
GKHDRHLNND ITLLSANEFY NDLDRKNDLI VIGGPPCQAY SKIGRAKLKS LGEERRQEND 

       130        140        150        160        170        180 
ARGKLYENFL DYALHVDANV IVMENVPEAV NYGGVNIPDT VCDILINKGY DAIWTVLNAA 

       190        200        210        220        230        240 
DFGVPQTRVR LFVMAIKKDI GKIKFIPEPT HKPHINVKRQ SVNVRWMQSE IRNSKYYKQP 

       250        260        270        280        290        300 
NIPDETLSDW VTVGDAIGDL PNLFPVYNQK YKNYKPMMMK EYKSPPQNTF QKLMRINNKV 

       310        320        330        340        350        360 
DKVTGNMFRN TKRDFSIFDK MEEGDNYLDA HNIAMSLLRK EMRKSGITKE NEFEYRLLKD 

       370        380        390        400        410        420 
RIVPPYSTEK FVEKWRKLSS DKPSHTLVAH LSTDTYSHLH PREPRGISVR EAARLQSFPD 

       430        440        450        460 
DFLFDCSMGA AFKQIGNAVP PLLAKAIAEA MKKNIQGGCI 

« Hide

References

[1]"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
Takami H., Takaki Y., Uchiyama I.
Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000028 Genomic DNA. Translation: BAC15292.1.
RefSeqNP_694258.1. NC_004193.1.

3D structure databases

ProteinModelPortalQ8EL95.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING221109.OB3336.

Protein family/group databases

REBASE6390. M.OihORF3336P.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC15292; BAC15292; BAC15292.
GeneID1018006.
KEGGoih:OB3336.
PATRIC22797780. VBIOceIhe82024_3394.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0270.
HOGENOMHOG000225505.
KOK00558.
OMAFRQIGNS.
OrthoDBEOG6KMB5M.

Enzyme and pathway databases

BioCycOIHE221109:GI2A-3427-MONOMER.

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMT36_OCEIH
AccessionPrimary (citable) accession number: Q8EL95
Entry history
Integrated into UniProtKB/Swiss-Prot: July 30, 2004
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families