Q8EL95 (MT36_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative modification methylase OB3336
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase
M.OihORF3336P

Gene names

Ordered Locus Names:

OB3336

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Oceanobacillus › Oceanobacillus iheyensis

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by a specific endonuclease Potential.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Putative modification methylase OB3336		PRO_0000087915
Sites
	Active site	97	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EL95 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5483B86933CDBD0C	FASTA	460	52,508
	10 20 30 40 50 60 MHQIYSATST DKKLPEVVDL FSGCGGLALG FQLAGFNIRK GIELDRDASD VASFNLHWRQ 70 80 90 100 110 120 GKHDRHLNND ITLLSANEFY NDLDRKNDLI VIGGPPCQAY SKIGRAKLKS LGEERRQEND 130 140 150 160 170 180 ARGKLYENFL DYALHVDANV IVMENVPEAV NYGGVNIPDT VCDILINKGY DAIWTVLNAA 190 200 210 220 230 240 DFGVPQTRVR LFVMAIKKDI GKIKFIPEPT HKPHINVKRQ SVNVRWMQSE IRNSKYYKQP 250 260 270 280 290 300 NIPDETLSDW VTVGDAIGDL PNLFPVYNQK YKNYKPMMMK EYKSPPQNTF QKLMRINNKV 310 320 330 340 350 360 DKVTGNMFRN TKRDFSIFDK MEEGDNYLDA HNIAMSLLRK EMRKSGITKE NEFEYRLLKD 370 380 390 400 410 420 RIVPPYSTEK FVEKWRKLSS DKPSHTLVAH LSTDTYSHLH PREPRGISVR EAARLQSFPD 430 440 450 460 DFLFDCSMGA AFKQIGNAVP PLLAKAIAEA MKKNIQGGCI

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC15292.1.
RefSeq	NP_694258.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8EL95.
ModBase	Search...
Protein-protein interaction databases
STRING	221109.OB3336.
Protein family/group databases
REBASE	6390. M.OihORF3336P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC15292; BAC15292; BAC15292.
GeneID	1018006.
KEGG	oih:OB3336.
PATRIC	22797780. VBIOceIhe82024_3394.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0270.
HOGENOM	HOG000225505.
KO	K00558.
OMA	WAWTLNT.
ProtClustDB	CLSK482557.
Enzyme and pathway databases
BioCyc	OIHE221109:GI2A-3427-MONOMER.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. False negative. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MT36_OCEIH

Accession

Primary (citable) accession number: Q8EL95

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 30, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program