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Q8EL95

- MT36_OCEIH

UniProt

Q8EL95 - MT36_OCEIH

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Protein

Putative modification methylase OB3336

Gene
OB3336
Organism
Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence ACCGGT, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by a specific endonuclease Reviewed prediction.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei97 – 971 By similarity

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciOIHE221109:GI2A-3427-MONOMER.

Protein family/group databases

REBASEi6390. M.OihORF3336P.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative modification methylase OB3336 (EC:2.1.1.37)
Alternative name(s):
Cytosine-specific methyltransferase
M.OihORF3336P
Gene namesi
Ordered Locus Names:OB3336
OrganismiOceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
ProteomesiUP000000822: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 460460Putative modification methylase OB3336PRO_0000087915Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi221109.OB3336.

Structurei

3D structure databases

ProteinModelPortaliQ8EL95.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 458444SAM-dependent MTase C5-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0270.
HOGENOMiHOG000225505.
KOiK00558.
OMAiFRQIGNS.
OrthoDBiEOG6KMB5M.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10629. PTHR10629. 1 hit.
PfamiPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EL95-1 [UniParc]FASTAAdd to Basket

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MHQIYSATST DKKLPEVVDL FSGCGGLALG FQLAGFNIRK GIELDRDASD    50
VASFNLHWRQ GKHDRHLNND ITLLSANEFY NDLDRKNDLI VIGGPPCQAY 100
SKIGRAKLKS LGEERRQEND ARGKLYENFL DYALHVDANV IVMENVPEAV 150
NYGGVNIPDT VCDILINKGY DAIWTVLNAA DFGVPQTRVR LFVMAIKKDI 200
GKIKFIPEPT HKPHINVKRQ SVNVRWMQSE IRNSKYYKQP NIPDETLSDW 250
VTVGDAIGDL PNLFPVYNQK YKNYKPMMMK EYKSPPQNTF QKLMRINNKV 300
DKVTGNMFRN TKRDFSIFDK MEEGDNYLDA HNIAMSLLRK EMRKSGITKE 350
NEFEYRLLKD RIVPPYSTEK FVEKWRKLSS DKPSHTLVAH LSTDTYSHLH 400
PREPRGISVR EAARLQSFPD DFLFDCSMGA AFKQIGNAVP PLLAKAIAEA 450
MKKNIQGGCI 460
Length:460
Mass (Da):52,508
Last modified:March 1, 2003 - v1
Checksum:i5483B86933CDBD0C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC15292.1.
RefSeqiNP_694258.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC15292; BAC15292; BAC15292.
GeneIDi1018006.
KEGGioih:OB3336.
PATRICi22797780. VBIOceIhe82024_3394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000028 Genomic DNA. Translation: BAC15292.1 .
RefSeqi NP_694258.1. NC_004193.1.

3D structure databases

ProteinModelPortali Q8EL95.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 221109.OB3336.

Protein family/group databases

REBASEi 6390. M.OihORF3336P.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC15292 ; BAC15292 ; BAC15292 .
GeneIDi 1018006.
KEGGi oih:OB3336.
PATRICi 22797780. VBIOceIhe82024_3394.

Phylogenomic databases

eggNOGi COG0270.
HOGENOMi HOG000225505.
KOi K00558.
OMAi FRQIGNS.
OrthoDBi EOG6KMB5M.

Enzyme and pathway databases

BioCyci OIHE221109:GI2A-3427-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10629. PTHR10629. 1 hit.
Pfami PF00145. DNA_methylase. 1 hit.
[Graphical view ]
PRINTSi PR00105. C5METTRFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
TIGRFAMsi TIGR00675. dcm. 1 hit.
PROSITEi PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
    Takami H., Takaki Y., Uchiyama I.
    Nucleic Acids Res. 30:3927-3935(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Entry informationi

Entry nameiMT36_OCEIH
AccessioniPrimary (citable) accession number: Q8EL95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 30, 2004
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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