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Protein

tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG

Gene

mnmG

Organism
Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.UniRule annotation

Cofactori

FADUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei126FAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei181FADUniRule annotation1
Binding sitei370FADUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 19FADUniRule annotation6
Nucleotide bindingi273 – 287NADUniRule annotationAdd BLAST15

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGUniRule annotation
Alternative name(s):
Glucose-inhibited division protein AUniRule annotation
Gene namesi
Name:mnmGUniRule annotation
Synonyms:gidAUniRule annotation
Ordered Locus Names:OB3490
OrganismiOceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
Proteomesi
  • UP000000822 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001171431 – 629tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmGAdd BLAST629

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB3490.

Structurei

3D structure databases

ProteinModelPortaliQ8EKU3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EKU3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYNAGNYDV IVVGAGHAGV ESAYAAARMG AKTLMLTLNL DMIAFMPCNP
60 70 80 90 100
SIGGPAKGIV VREIDALGGI MGKVIDKSYI QMRMLNTGKG PAVQALRAQA
110 120 130 140 150
DKPIYMKEMK HLMENEENLT IRQGMVNELI VEDGICKGVI TETHAAYYAD
160 170 180 190 200
SVIVTTGTFM RGKVLMGDLE YESGPNNQRV SIKLSEDLEK HGFEITRFKT
210 220 230 240 250
GTPPRVNSHT IDYSKTEIQP GDDNPKSFSY ETTEQITDQI PCWLTYTNEF
260 270 280 290 300
THQVINDNLE LSSMYSGMKR GTGPRYCPSI EDKIVRFNDK PRHQIFLEPE
310 320 330 340 350
GRDTEEVYVQ GLSTSLPESI QHEMIKSVPG LEKAEIMRAG YAIEYDAVVP
360 370 380 390 400
TQLWPTLETK RLPGLFTAGQ INGTSGYEEA AGQGLMAGMN AAAKSLNKEP
410 420 430 440 450
IILDRSQAYI GVMIDDLVTK GTNEPYRLLT SRAEYRLLLR HDNADLRLTD
460 470 480 490 500
IGYDYGLITE ERYQRFTEKK QQIEQEKKRL SKIIIKPTED IQDIMEKAGA
510 520 530 540 550
SRLKEAIKAY DLMKRPELSY DIVDQMIESN LELSEEVKEQ VAIQIKYEGY
560 570 580 590 600
IKKAKEQVDR MLKMEDKKIP EDIDYDAING IATEAVEKLK KVRPLSVGQA
610 620
SRISGVNPAD VSILLVYIEQ GSIAKVAND
Length:629
Mass (Da):70,375
Last modified:March 1, 2003 - v1
Checksum:i9826EB392AE33DB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC15446.1.
RefSeqiWP_011067888.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC15446; BAC15446; BAC15446.
KEGGioih:OB3490.
PATRICi22798086. VBIOceIhe82024_3547.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC15446.1.
RefSeqiWP_011067888.1. NC_004193.1.

3D structure databases

ProteinModelPortaliQ8EKU3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi221109.OB3490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC15446; BAC15446; BAC15446.
KEGGioih:OB3490.
PATRICi22798086. VBIOceIhe82024_3547.

Phylogenomic databases

eggNOGiENOG4107RE5. Bacteria.
COG0445. LUCA.
HOGENOMiHOG000201060.
KOiK03495.
OMAiFRPGYAI.
OrthoDBiPOG091H01O0.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00129. MnmG_GidA. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR026904. GidA-assoc_3.
IPR004416. MnmG.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
PF13932. GIDA_assoc. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR00136. gidA. 1 hit.
PROSITEiPS01280. GIDA_1. 1 hit.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNMG_OCEIH
AccessioniPrimary (citable) accession number: Q8EKU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: March 1, 2003
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.