Q8EKR0 (RSMB_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176

Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB

Gene names

Name:	rsmB
Synonyms:	rrmB, sun
Ordered Locus Names:	SO_0030

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
Biological process	Ribosome biogenesis rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA methylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Ribosomal RNA small subunit methyltransferase B		PRO_0000211804
Regions
	Region	248 – 254	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	369	1	Nucleophile Potential
	Binding site	271	1	S-adenosyl-L-methionine By similarity
	Binding site	297	1	S-adenosyl-L-methionine By similarity
	Binding site	316	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EKR0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D5A60024FE6955E0	FASTA	428	47,797
	10 20 30 40 50 60 MNLRALAAKA IFDVLEKGVS LSVALPEQQK HLASGKDKAL LAELCYGVMR TLPQIEKRTA 70 80 90 100 110 120 ECLAKPLKGK QRIIHQLLIV GCYQLYFTRI PSHAAISETA EACRQLKFEG MVKVVNGVLR 130 140 150 160 170 180 NIQRQLTPLS NESETLSYNT PGWLIKRLKT AYPGNWQEII QQSHERPPMW LRNNRLSQSR 190 200 210 220 230 240 DEYLAALDKL EIDASAGLSD DAILLAAPKD VATLPLFLEG AASVQDGAAQ WAATLLAPQA 250 260 270 280 290 300 NELILDACAA PGGKSCHLLE LEPSIKLIAV DFDAKRLERV QQNLDRLSLK AEVIHGDAAN 310 320 330 340 350 360 IDSWWQGEQF DRILLDAPCS ATGVIRRHPD IKWLRKNHDI EELAELQRQI LDHCWKWLKP 370 380 390 400 410 420 GGTLLYATCS ILPQENRDQI SAFLARTADA KLDTLVQQAS SQDIGWQITP GQHNMDGFYY ARLLKATH

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN53117.1.
RefSeq	NP_715672.1. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EKR0.
SMR	Q8EKR0. Positions 2-425.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_0030.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN53117; AAN53117; SO_0030.
GeneID	1167928.
KEGG	son:SO_0030.
PATRIC	23519751. VBISheOne101494_0030.
Phylogenomic databases
eggNOG	COG0144.
HOGENOM	HOG000037300.
KO	K03500.
OMA	KPPMWLR.
ProtClustDB	PRK10901.
Family and domain databases
Gene3D	1.10.940.10. 1 hit.
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. IPR006027. NusB_RsmB_TIM44. IPR023267. RCMT. IPR004573. rRNA_ssu_MeTfrase_B. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. PF01029. NusB. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY.
SUPFAM	SSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMs	TIGR00563. rsmB. 1 hit.
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMB_SHEON

Accession

Primary (citable) accession number: Q8EKR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program