Reviewed, UniProtKB/Swiss-Prot Q8EK44 (CCME_SHEON)

Names and origin

Protein names

Recommended name:
    Cytochrome c-type biogenesis protein ccmE
Alternative name(s):
    Cytochrome c maturation protein E
    Heme chaperone ccmE

Gene names

Name:	ccmE
Synonyms:	cycJ
Ordered Locus Names:	SO_0259

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by ccmC and transfers the heme to apo-cytochromes in a process facilitated by ccmF and ccmH By similarity.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein; Periplasmic side Potential.
Sequence similarities	Belongs to the ccmE/cycJ family.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal-anchor Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW protein-heme linkage Inferred from electronic annotation. Source: HAMAP
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 161

161

Cytochrome c-type biogenesis protein ccmE HAMAP MF_01959

PRO_0000238864

Regions

Topological domain

1 – 8

8

Cytoplasmic Potential

Transmembrane

9 – 29

21

Signal-anchor for type II membrane protein Potential

Topological domain

30 – 161

132

Periplasmic Potential

Sites

Metal binding

135

1

Iron (heme axial ligand) By similarity

Binding site

131

1

Heme (covalent; via pros nitrogen) By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

161

Helix Strand Turn

Details...

Turn

40 – 46

7

Turn

48 – 51

4

Beta strand

52 – 54

3

Beta strand

59 – 66

8

Beta strand

80 – 85

6

Beta strand

92 – 96

5

Beta strand

106 – 116

11

Beta strand

121 – 128

8

Sequences

	Sequence		Length	Mass (Da)
	Q8EK44-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9B7FB4604F2A0629	FASTA	161	17,358
	10 20 30 40 50 60 MNPRRKKRLT LAVALIGGVA AIASLLLYAL NSNLNLFYTP SEIVNGKTDT GVKPEAGQRI 70 80 90 100 110 120 RVGGMVTVGS MVRDPNSLHV QFAVHDSLGG EILVTYDDLL PDLFREGQGI VAQGVLGEDG 130 140 150 160 KLAATEVLAK HDENYMPPEV AEAMGQKHEK LDYSQQKSAT Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cytochrome c maturation in the metal reducer Shewanella putrefaciens." Saffarini D.A., McCool J., Tsongalis J. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MR-1.
[2]	"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.
[3]	"Solution structure and characterization of the heme chaperone CcmE." Arnesano F., Banci L., Barker P.D., Bertini I., Rosato A., Su X.C., Viezzoli M.S. Biochemistry 41:13587-13594(2002) [PubMed: 12427019] [Abstract] Cited for: STRUCTURE BY NMR OF 30-161.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AF044582 Genomic DNA. Translation: AAC02697.1.
AE014299 Genomic DNA. Translation: AAN53344.1.

RefSeq

NP_715899.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J6Q	NMR	-	A	30-161	[»]
1LM0	NMR	-	A	30-161	[»]

ModBase

Search...

Genome annotation databases

GeneID

1168143.

GenomeReviews

Gene locus SO_0259 in contig AE014299_GR.

KEGG

son:SO_0259.

NMPDR

fig|211586.1.peg.244.

TIGR

SO_0259.

Phylogenomic databases

HOGENOM

Q8EK44.

OMA

VEKGSLQ.

Enzyme and pathway databases

BioCyc

SONE211586:SO_0259-MON.

Family and domain databases

HAMAP

MF_01959.
[Tree]

InterPro

IPR004329. CcmE.
IPR012340. NA-bd_OB-fold.
[Graphical view]

Gene3D

G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.

Pfam

PF03100. CcmE. 1 hit.
[Graphical view]

ProDom

PD006742. CcmE. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

CCME_SHEON

Accession

Primary (citable) accession number: Q8EK44
Secondary accession number(s): O52690

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)