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Protein

Cytochrome c-type biogenesis protein CcmE

Gene

ccmE

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311Heme (covalent; via pros nitrogen)By similarity
Metal bindingi135 – 1351Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cytochrome c-type biogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c-type biogenesis protein CcmE
Alternative name(s):
Cytochrome c maturation protein E
Heme chaperone CcmE
Gene namesi
Name:ccmE
Synonyms:cycJ
Ordered Locus Names:SO_0259
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 161132PeriplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Cytochrome c-type biogenesis protein CcmEPRO_0000238864Add
BLAST

Proteomic databases

PaxDbiQ8EK44.

Interactioni

Protein-protein interaction databases

STRINGi211586.SO_0259.

Structurei

Secondary structure

1
161
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni40 – 467Combined sources
Turni48 – 514Combined sources
Beta strandi52 – 543Combined sources
Beta strandi59 – 668Combined sources
Beta strandi80 – 856Combined sources
Beta strandi92 – 965Combined sources
Helixi101 – 1033Combined sources
Beta strandi106 – 11611Combined sources
Beta strandi121 – 1288Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J6QNMR-A30-161[»]
1LM0NMR-A30-161[»]
ProteinModelPortaliQ8EK44.
SMRiQ8EK44. Positions 32-132.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8EK44.

Family & Domainsi

Sequence similaritiesi

Belongs to the CcmE/CycJ family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZKK. Bacteria.
COG2332. LUCA.
HOGENOMiHOG000009661.
KOiK02197.
OMAiHVEFAVH.
OrthoDBiEOG69D3JN.
PhylomeDBiQ8EK44.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_01959. CcmE.
InterProiIPR004329. CcmE.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF03100. CcmE. 1 hit.
[Graphical view]
SUPFAMiSSF82093. SSF82093. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8EK44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPRRKKRLT LAVALIGGVA AIASLLLYAL NSNLNLFYTP SEIVNGKTDT
60 70 80 90 100
GVKPEAGQRI RVGGMVTVGS MVRDPNSLHV QFAVHDSLGG EILVTYDDLL
110 120 130 140 150
PDLFREGQGI VAQGVLGEDG KLAATEVLAK HDENYMPPEV AEAMGQKHEK
160
LDYSQQKSAT Q
Length:161
Mass (Da):17,358
Last modified:March 1, 2003 - v1
Checksum:i9B7FB4604F2A0629
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044582 Genomic DNA. Translation: AAC02697.1.
AE014299 Genomic DNA. Translation: AAN53344.1.
RefSeqiNP_715899.1. NC_004347.2.
WP_011070633.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN53344; AAN53344; SO_0259.
GeneIDi1168143.
KEGGison:SO_0259.
PATRICi23520217. VBISheOne101494_0250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF044582 Genomic DNA. Translation: AAC02697.1.
AE014299 Genomic DNA. Translation: AAN53344.1.
RefSeqiNP_715899.1. NC_004347.2.
WP_011070633.1. NC_004347.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1J6QNMR-A30-161[»]
1LM0NMR-A30-161[»]
ProteinModelPortaliQ8EK44.
SMRiQ8EK44. Positions 32-132.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi211586.SO_0259.

Proteomic databases

PaxDbiQ8EK44.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN53344; AAN53344; SO_0259.
GeneIDi1168143.
KEGGison:SO_0259.
PATRICi23520217. VBISheOne101494_0250.

Phylogenomic databases

eggNOGiENOG4108ZKK. Bacteria.
COG2332. LUCA.
HOGENOMiHOG000009661.
KOiK02197.
OMAiHVEFAVH.
OrthoDBiEOG69D3JN.
PhylomeDBiQ8EK44.

Miscellaneous databases

EvolutionaryTraceiQ8EK44.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_01959. CcmE.
InterProiIPR004329. CcmE.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF03100. CcmE. 1 hit.
[Graphical view]
SUPFAMiSSF82093. SSF82093. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cytochrome c maturation in the metal reducer Shewanella putrefaciens."
    Saffarini D.A., McCool J., Tsongalis J.
    Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MR-1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.
  3. "Solution structure and characterization of the heme chaperone CcmE."
    Arnesano F., Banci L., Barker P.D., Bertini I., Rosato A., Su X.C., Viezzoli M.S.
    Biochemistry 41:13587-13594(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 30-161.

Entry informationi

Entry nameiCCME_SHEON
AccessioniPrimary (citable) accession number: Q8EK44
Secondary accession number(s): O52690
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 1, 2003
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.