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Q8EK12 (SYW_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:SO_0294
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136673

Regions

Motif12 – 209"HIGH" region HAMAP-Rule MF_00140
Motif195 – 1995"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site1981ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EK12 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 901DE9700C80C3B0

FASTA33236,769
        10         20         30         40         50         60 
MTKPIVLSGA QPSGELTIGN YMGALRQWVA MQDSHDCLYC VVDLHAITVR QDPQALREAC 

        70         80         90        100        110        120 
LDTLALYLAC GVDPKKSTVF IQSQVPQHTQ LGWALNCYTQ MGELSRMTQF KDKSQKHANN 

       130        140        150        160        170        180 
INVGLFGYPV LMAADILLYQ ANEIPVGQDQ KQHLELTRDI ATRFNNAYGE TFTIPEPFIP 

       190        200        210        220        230        240 
EHGAKVMSLQ DPLKKMSKSD DNRNNVIGLL EDPKAVMKKL KKAMTDSDEP PVVRFDIENK 

       250        260        270        280        290        300 
PGVSNLLSLM SGITGQSIAS LEAEFEGKMY GHLKGAAGEA VVGMLEPLQE RYRALRADRA 

       310        320        330 
YLDQVMRAGA ENAQARAEVT LKKVYEKIGL LV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN53379.1.
RefSeqNP_715934.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8EK12.
SMRQ8EK12. Positions 5-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_0294.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN53379; AAN53379; SO_0294.
GeneID1168176.
KEGGson:SO_0294.
PATRIC23520295. VBISheOne101494_0286.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMALLNCYTY.
OrthoDBEOG686NJQ.
PhylomeDBQ8EK12.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_SHEON
AccessionPrimary (citable) accession number: Q8EK12
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries