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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei135L-tryptophanUniRule annotation1
Binding sitei186ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 13ATPUniRule annotation3
Nucleotide bindingi19 – 20ATPUniRule annotation2
Nucleotide bindingi147 – 149ATPUniRule annotation3
Nucleotide bindingi195 – 199ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSONE211586:G1GMP-284-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:SO_0294
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366731 – 332Tryptophan--tRNA ligaseAdd BLAST332

Proteomic databases

PaxDbiQ8EK12

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_0294

Structurei

3D structure databases

ProteinModelPortaliQ8EK12
SMRiQ8EK12
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 20"HIGH" regionUniRule annotation9
Motifi195 – 199"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9
PhylomeDBiQ8EK12

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8EK12-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKPIVLSGA QPSGELTIGN YMGALRQWVA MQDSHDCLYC VVDLHAITVR
60 70 80 90 100
QDPQALREAC LDTLALYLAC GVDPKKSTVF IQSQVPQHTQ LGWALNCYTQ
110 120 130 140 150
MGELSRMTQF KDKSQKHANN INVGLFGYPV LMAADILLYQ ANEIPVGQDQ
160 170 180 190 200
KQHLELTRDI ATRFNNAYGE TFTIPEPFIP EHGAKVMSLQ DPLKKMSKSD
210 220 230 240 250
DNRNNVIGLL EDPKAVMKKL KKAMTDSDEP PVVRFDIENK PGVSNLLSLM
260 270 280 290 300
SGITGQSIAS LEAEFEGKMY GHLKGAAGEA VVGMLEPLQE RYRALRADRA
310 320 330
YLDQVMRAGA ENAQARAEVT LKKVYEKIGL LV
Length:332
Mass (Da):36,769
Last modified:March 1, 2003 - v1
Checksum:i901DE9700C80C3B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA Translation: AAN53379.1
RefSeqiNP_715934.1, NC_004347.2
WP_011070666.1, NC_004347.2

Genome annotation databases

EnsemblBacteriaiAAN53379; AAN53379; SO_0294
GeneIDi1168176
KEGGison:SO_0294
PATRICifig|211586.12.peg.286

Similar proteinsi

Entry informationi

Entry nameiSYW_SHEON
AccessioniPrimary (citable) accession number: Q8EK12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: April 25, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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