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Protein

tRNA-dihydrouridine synthase B

Gene

dusB

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines.UniRule annotation

Catalytic activityi

5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H.UniRule annotation

Cofactori

FMNUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701FMNUniRule annotation
Active sitei100 – 1001Proton donorUniRule annotation
Binding sitei139 – 1391FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi16 – 183FMNUniRule annotation
Nucleotide bindingi200 – 2023FMNUniRule annotation
Nucleotide bindingi224 – 2252FMNUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Flavoprotein, FMN, NADP, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-dihydrouridine synthase BUniRule annotation (EC:1.3.1.-UniRule annotation)
Gene namesi
Name:dusBUniRule annotation
Ordered Locus Names:SO_0394
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322tRNA-dihydrouridine synthase BPRO_0000162099Add
BLAST

Proteomic databases

PaxDbiQ8EJR8.

Interactioni

Protein-protein interaction databases

STRINGi211586.SO_0394.

Structurei

3D structure databases

ProteinModelPortaliQ8EJR8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Dus family. DusB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CEH. Bacteria.
COG0042. LUCA.
HOGENOMiHOG000217855.
KOiK05540.
OMAiWLFREIE.
OrthoDBiEOG6VHZFQ.
PhylomeDBiQ8EJR8.

Family and domain databases

Gene3Di1.10.1200.80. 1 hit.
3.20.20.70. 1 hit.
HAMAPiMF_02042. DusB_subfam.
InterProiIPR013785. Aldolase_TIM.
IPR032887. DusB.
IPR024036. tRNA-dHydroUridine_Synthase_C.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
TIGRFAMsiTIGR00737. nifR3_yhdG. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EJR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIGPYQLKN QLIVAPMAGV TDQAFRNLCL RYGAALAVSE MLSSNPEVWD
60 70 80 90 100
TDKSRQRMTH SGEEGIRSVQ IAGADPELMA QAAQFNVEQG AHIIDINMGC
110 120 130 140 150
PAKKVNKKLA GSALMQNPPL VKDILQAVVA AVDVPVTLKI RTGWEPEHRN
160 170 180 190 200
GVQIAQIAED CGIASLAVHG RTRQCMYKGN AEYDTIKAIK QNVSIPVVAN
210 220 230 240 250
GDIDSPEKAR FVLDYTGVDA LMIGRGAQGR PWIFREIQHY LETGNKLAPI
260 270 280 290 300
EVAEQRQVML EHLTKLYDLY GEYKGIRFAR KHIGWYLDQE DQRQFRADFN
310 320
QLETAAEQYS LVEYYFDELV QN
Length:322
Mass (Da):36,080
Last modified:March 1, 2003 - v1
Checksum:i16C834A76A454CC9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA. Translation: AAN53477.1.
RefSeqiNP_716032.1. NC_004347.2.
WP_011070754.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN53477; AAN53477; SO_0394.
GeneIDi1168271.
KEGGison:SO_0394.
PATRICi23520491. VBISheOne101494_0384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA. Translation: AAN53477.1.
RefSeqiNP_716032.1. NC_004347.2.
WP_011070754.1. NC_004347.2.

3D structure databases

ProteinModelPortaliQ8EJR8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi211586.SO_0394.

Proteomic databases

PaxDbiQ8EJR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN53477; AAN53477; SO_0394.
GeneIDi1168271.
KEGGison:SO_0394.
PATRICi23520491. VBISheOne101494_0384.

Phylogenomic databases

eggNOGiENOG4105CEH. Bacteria.
COG0042. LUCA.
HOGENOMiHOG000217855.
KOiK05540.
OMAiWLFREIE.
OrthoDBiEOG6VHZFQ.
PhylomeDBiQ8EJR8.

Family and domain databases

Gene3Di1.10.1200.80. 1 hit.
3.20.20.70. 1 hit.
HAMAPiMF_02042. DusB_subfam.
InterProiIPR013785. Aldolase_TIM.
IPR032887. DusB.
IPR024036. tRNA-dHydroUridine_Synthase_C.
IPR004652. tRNA_dU_NifR3.
IPR001269. tRNA_hU_synthase.
IPR018517. tRNA_hU_synthase_CS.
[Graphical view]
PANTHERiPTHR11082. PTHR11082. 1 hit.
PfamiPF01207. Dus. 1 hit.
[Graphical view]
PIRSFiPIRSF006621. Dus. 1 hit.
TIGRFAMsiTIGR00737. nifR3_yhdG. 1 hit.
PROSITEiPS01136. UPF0034. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiDUSB_SHEON
AccessioniPrimary (citable) accession number: Q8EJR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: April 13, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.