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Q8EJM1 (PUR9_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:SO_0442
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 543543Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_0000192121

Sequences

Sequence LengthMass (Da)Tools
Q8EJM1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7A6AB5179A4D961E

FASTA54358,221
        10         20         30         40         50         60 
MTVANHARPI RRALLSVSDK TGILEFAKAL HAQGVELLST GGTARLLADN GVPVIEVSDY 

        70         80         90        100        110        120 
TGHPEIMDGR VKTLHPKVHG GILARRGLDE NVMAANNINA IDLVAVNLYP FADTVAKAGC 

       130        140        150        160        170        180 
TLEDAIENID IGGPTMVRAA AKNHKDVTIV VNAADYNRVL AEMAVNNGST THATRFDLAI 

       190        200        210        220        230        240 
AAFEHTAGYD GMIANYFGTM VPMHRVPAHS TDECFQDSLS VEGSKFPRTF NTQLVKKQDL 

       250        260        270        280        290        300 
RYGENSHQAA AFYVDTKIDE ASVATAVQLQ GKALSYNNIA DTDAALECVK EFSEPACVIV 

       310        320        330        340        350        360 
KHANPCGVAL GKDLLDAYNR AYQTDPTSAF GGIIAFNGEL DAATASAIVE RQFVEVIIAP 

       370        380        390        400        410        420 
VVSQGARDVV AKKTNVRLLE CGQWNTKTQT LDYKRVNGGL LVQDRDQGMV GLDDIKVVTK 

       430        440        450        460        470        480 
RQPTESELKD LMFCWKVAKF VKSNAIVYAK DGMTIGVGAG QMSRVYSAKI AGIKAADEGL 

       490        500        510        520        530        540 
EVVNSVMASD AFFPFRDGID AAAAAGISCI IQPGGSMRDA EIIAAADEHG MAMVMTGMRH 


FRH 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN53524.1.
RefSeqNP_716079.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8EJM1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_0442.

Proteomic databases

PRIDEQ8EJM1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN53524; AAN53524; SO_0442.
GeneID1168318.
KEGGson:SO_0442.
PATRIC23520585. VBISheOne101494_0431.

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMADLLFAWK.
OrthoDBEOG6QCDFF.
ProtClustDBPRK00881.

Enzyme and pathway databases

UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_SHEON
AccessionPrimary (citable) accession number: Q8EJM1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways