ID   FTHS_SHEON              Reviewed;         570 AA.
AC   Q8EJA7;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   27-MAR-2024, entry version 106.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=SO_0560;
OS   Shewanella oneidensis (strain MR-1).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=211586;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A.,
RA   Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C.,
RA   DeBoy R.T., Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R.,
RA   Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J.,
RA   Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J.,
RA   Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V.,
RA   Smith H.O., Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AE014299; AAN53641.1; -; Genomic_DNA.
DR   RefSeq; NP_716196.1; NC_004347.2.
DR   RefSeq; WP_011070900.1; NZ_CP053946.1.
DR   AlphaFoldDB; Q8EJA7; -.
DR   SMR; Q8EJA7; -.
DR   STRING; 211586.SO_0560; -.
DR   PaxDb; 211586-SO_0560; -.
DR   KEGG; son:SO_0560; -.
DR   PATRIC; fig|211586.12.peg.539; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   OrthoDB; 9761733at2; -.
DR   PhylomeDB; Q8EJA7; -.
DR   BioCyc; SONE211586:G1GMP-531-MONOMER; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000008186; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..570
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199373"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   570 AA;  60232 MW;  CD7C2D1DA2626AEB CRC64;
     MLTDMEISRR AHLKDIAQLG AEFGLLPEEM QLFGNTKAKV DLQVQQRLAG QPKGKLIIVT
     AVTPTPHGEG KTVTTIGLTQ SLKALGNKVC ACIRQPSMGP VFGVKGGAAG GGYAQVVPMQ
     ELNLHLTGDI HAVSSAHNLG AAAIASRLYH EMRLGKAEFE RQSGLSYLDI DPKGIRWHRV
     VDHNDRCLRE IEVGLGENNG PAYTSGFDIT AASELMAILA LSRNLTDMRV RIGKLVLALN
     RQGEAVSADE LGVAGAMTAI MADAVKPTLM QTLNGAPCLI HAGPFANIAH GNSSVIADDI
     ALKLADFVVT EGGFGSDMGF EKFCNIKARQ SGLIPSAAVL VTTLKALKAN SGLASDTDIN
     APDQARLEAG FANLNWHINN VVQYGLPVVV AINRFASDTD AELNWLMEAV RGTAAFGCEL
     SEAFSQGEDG AMALAQTVIR ACEQPSEFRL LYPDNMELEA KLSTLAELGY GAAGINLSAV
     AKQQLQELSA LGYAHLPVCM AKTPLSISHD PQLKGVPQGF IVPVRELVLN AGAGFITALV
     GNVMTMPGLG LTPGYLKIDI DVDGEIIGLG
//