Q8EIV5 (KATG2_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase 2
Short name=CP 2
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	SO_0725

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 741	714	Catalase-peroxidase 2 HAMAP MF_01961		PRO_0000354921
Sites
	Active site	107	1	Proton acceptor By similarity
	Metal binding	270	1	Iron (heme axial ligand) By similarity
	Site	103	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	106 ↔ 229		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255) By similarity
	Cross-link	229 ↔ 255		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-106) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EIV5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 2318B8A0E9567F1F	FASTA	741	81,256
	10 20 30 40 50 60 MKINTLPTLS ALTLAMSLAL GAGMATAQEQ ATGNQFWWPE KLNLSPLRQN AIESNPYGSD 70 80 90 100 110 120 YRYAEAFNTL DLDAVKKDIK ALMTESQDWW PADYGHYGPF FIRMAWHSAG VYRIFDGRGG 130 140 150 160 170 180 AAGGQQRFEP LNSWPADNVS LDKARRLLWP IKQKYGSKLS WGDLMVLTGN VALESMGFKT 190 200 210 220 230 240 FGFGGGRVDD WEAEMVNWGS EKAWLDNKRH NGKGELAKPM GATQMGLIYV NPEGPNGVPD 250 260 270 280 290 300 PLASAKEIRD TFGRMAMNDE ETVALIAGGH TFGKAHGAHD PAKCVGADPA AAGIEEQGLG 310 320 330 340 350 360 WKNKCGKGHS EDTVTSGLEG AWSSNPTKWT MEYLTWLYTF DWVQTKSPAG HIQWTPADGK 370 380 390 400 410 420 AANLVPDAHL PDKRHAPIMF TSDIALKADP IYREITTRFL KNPQEFELAF AKAWFKLTHR 430 440 450 460 470 480 DLGPKARYLG ADVPAEALIW QDPIPALDHP LIDNADIKAL GNKILASGLT VPELVRTAWA 490 500 510 520 530 540 SASSFRGTDM RGGANGARIR LEPMMNWQAN NPKELAKVLA KLEKVQKDFN GSLKGSKKVS 550 560 570 580 590 600 LADVIVLGGS VAVEKAAKEA GVVISVPFTP GRMDATQVQT DVNSFAVLEP AADGFRNYYS 610 620 630 640 650 660 KDSSLSPAEM LIERANMLNL TVPEMTVLVG GLRALDANSA GVKHGVFTDK PGVLSNDFFV 670 680 690 700 710 720 NLLDMSTKWR KSDKQEGIYE GQDRNSGKLK WTATPVDLIF GSHSELRAVS EVYGAQDGQD 730 740 RFIQDFVKAW NKVMNADRFD I

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN53803.1.
RefSeq	NP_716358.1. NC_004347.1.
3D structure databases
HSSP	HSSP built from PDB template 1ITK based on UniProtKB O59651.
ProteinModelPortal	Q8EIV5.
SMR	Q8EIV5. Positions 31-741.
ModBase	Search...
Protein family/group databases
PeroxiBase	2444. SonCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1168580.
GenomeReviews	Gene locus SO_0725 in contig AE014299_GR.
KEGG	son:SO_0725.
NMPDR	fig|211586.1.peg.675.
PATRIC	23521120. VBISheOne101494_0694.
TIGR	SO_0725.
Phylogenomic databases
HOGENOM	HBG285610.
OMA	KNKCGKG.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	SONE211586:SO_0725-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_SHEON

Accession

Primary (citable) accession number: Q8EIV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program