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Q8EIJ1

- NAPA_SHEON

UniProt

Q8EIJ1 - NAPA_SHEON

Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster.UniRule annotation
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. electron carrier activity Source: UniProtKB-HAMAP
    3. iron ion binding Source: UniProtKB-HAMAP
    4. molybdenum ion binding Source: InterPro
    5. nitrate reductase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
    2. nitrate assimilation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
    Gene namesi
    Name:napAUniRule annotation
    Ordered Locus Names:SO_0848
    OrganismiShewanella oneidensis (strain MR-1)
    Taxonomic identifieri211586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000008186: Chromosome

    Subcellular locationi

    Periplasm UniRule annotation

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
    BLAST
    Chaini33 – 826794Periplasmic nitrate reductasePRO_0000046003Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

    Interactioni

    Subunit structurei

    Interacts with NapB.UniRule annotation

    Protein-protein interaction databases

    STRINGi211586.SO_0848.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8EIJ1.
    SMRiQ8EIJ1. Positions 38-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 94574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0243.
    HOGENOMiHOG000031441.
    KOiK02567.
    OMAiRRLSHKD.
    OrthoDBiEOG6CVV7G.
    PhylomeDBiQ8EIJ1.

    Family and domain databases

    HAMAPiMF_01630. Nitrate_reduct.
    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view]
    PfamiPF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8EIJ1-1 [UniParc]FASTAAdd to Basket

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    MSISRREFLK ANAAVAAATA VGVTLPVKMV EAAESDNIKW DKAPCRFCGV    50
    GCSVLVGTKA GKVVATKGDP ESPVNRGLNC IKGYFLSKIM YGKDRLTTPL 100
    LRMKDGKYHK EGEFTPVSWD VAFDTMAAKW KHSIATKGPT SVGMFGSGQW 150
    TIWEGYAASK LHKAGFLTNN IDPNARHCMA SAVVGFMRTF GIDEPMGCYD 200
    DLEAADHFVL WGANMAEMHP ILWARLSDRR LSSKDCRVHV LSTFENRSFD 250
    LADNPMVFHP QSDLVILNYI ANYIIQNKAV NTDFVTKHTK FALGVDDIGY 300
    GLRPDHPLEK KAKNPGNGKS TPISFDEYAK FVSTYTLEYA AKMSGVEPEK 350
    LETLAKAYAD PKAKVMSLWT MGINQHVRGV WANNMLYNIH LLTGKIATPG 400
    NSPFSLTGQP SACGTAREVG TFAHRLPADM VVDNDKHRAI TEKMWQVPEG 450
    TIPPKPGYHA VLQSRMLKDG KLNCYWTMCT NNMQAGPNIN EEMYPGFRNP 500
    ENFIVVSDPY PTVTAMAADL ILPTAMWVEK EGAYGNAERR THMWHQQVKA 550
    PEGAKSDLWQ LVEFSKRFKV AEVWPAELIA KKPEYADKTL YEVLFANGVI 600
    NKFPTTDCKG DLNDESEHFS FYVQKGIFEE YAAFGRGHGH DLAEFDRYHE 650
    TRGLRWPVVN DKETLRRFVE GSDPYVKAGE GYNFYGKPDG KAVIFALPYE 700
    PAAEEPNSEY DLWMSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED 750
    AKARGLQRGD EVLVASPRGE IKTRVETKGR NKPPRGVVFM PFFDARQLVN 800
    KLILDATDPL SKETDFKKCP VKVMKA 826
    Length:826
    Mass (Da):92,475
    Last modified:March 1, 2003 - v1
    Checksum:i33474D23868F6316
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN53924.1.
    RefSeqiNP_716479.1. NC_004347.2.
    WP_011071138.1. NC_004347.2.

    Genome annotation databases

    EnsemblBacteriaiAAN53924; AAN53924; SO_0848.
    GeneIDi1168695.
    KEGGison:SO_0848.
    PATRICi23521357. VBISheOne101494_0812.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN53924.1 .
    RefSeqi NP_716479.1. NC_004347.2.
    WP_011071138.1. NC_004347.2.

    3D structure databases

    ProteinModelPortali Q8EIJ1.
    SMRi Q8EIJ1. Positions 38-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211586.SO_0848.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN53924 ; AAN53924 ; SO_0848 .
    GeneIDi 1168695.
    KEGGi son:SO_0848.
    PATRICi 23521357. VBISheOne101494_0812.

    Phylogenomic databases

    eggNOGi COG0243.
    HOGENOMi HOG000031441.
    KOi K02567.
    OMAi RRLSHKD.
    OrthoDBi EOG6CVV7G.
    PhylomeDBi Q8EIJ1.

    Family and domain databases

    HAMAPi MF_01630. Nitrate_reduct.
    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR010051. Periplasm_NO3_reductase_lsu.
    IPR006311. TAT_signal.
    IPR019546. TAT_signal_bac_arc.
    [Graphical view ]
    Pfami PF04879. Molybdop_Fe4S4. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01706. NAPA. 1 hit.
    TIGR01409. TAT_signal_seq. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS51318. TAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MR-1.

    Entry informationi

    Entry nameiNAPA_SHEON
    AccessioniPrimary (citable) accession number: Q8EIJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 7, 2006
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3