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Q8EIJ1 (NAPA_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:SO_0848
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length826 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232Tat-type signal Potential
Chain33 – 826794Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000046003

Regions

Domain38 – 94574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding451Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding521Iron-sulfur (4Fe-4S) By similarity
Metal binding801Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EIJ1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 33474D23868F6316

FASTA82692,475
        10         20         30         40         50         60 
MSISRREFLK ANAAVAAATA VGVTLPVKMV EAAESDNIKW DKAPCRFCGV GCSVLVGTKA 

        70         80         90        100        110        120 
GKVVATKGDP ESPVNRGLNC IKGYFLSKIM YGKDRLTTPL LRMKDGKYHK EGEFTPVSWD 

       130        140        150        160        170        180 
VAFDTMAAKW KHSIATKGPT SVGMFGSGQW TIWEGYAASK LHKAGFLTNN IDPNARHCMA 

       190        200        210        220        230        240 
SAVVGFMRTF GIDEPMGCYD DLEAADHFVL WGANMAEMHP ILWARLSDRR LSSKDCRVHV 

       250        260        270        280        290        300 
LSTFENRSFD LADNPMVFHP QSDLVILNYI ANYIIQNKAV NTDFVTKHTK FALGVDDIGY 

       310        320        330        340        350        360 
GLRPDHPLEK KAKNPGNGKS TPISFDEYAK FVSTYTLEYA AKMSGVEPEK LETLAKAYAD 

       370        380        390        400        410        420 
PKAKVMSLWT MGINQHVRGV WANNMLYNIH LLTGKIATPG NSPFSLTGQP SACGTAREVG 

       430        440        450        460        470        480 
TFAHRLPADM VVDNDKHRAI TEKMWQVPEG TIPPKPGYHA VLQSRMLKDG KLNCYWTMCT 

       490        500        510        520        530        540 
NNMQAGPNIN EEMYPGFRNP ENFIVVSDPY PTVTAMAADL ILPTAMWVEK EGAYGNAERR 

       550        560        570        580        590        600 
THMWHQQVKA PEGAKSDLWQ LVEFSKRFKV AEVWPAELIA KKPEYADKTL YEVLFANGVI 

       610        620        630        640        650        660 
NKFPTTDCKG DLNDESEHFS FYVQKGIFEE YAAFGRGHGH DLAEFDRYHE TRGLRWPVVN 

       670        680        690        700        710        720 
DKETLRRFVE GSDPYVKAGE GYNFYGKPDG KAVIFALPYE PAAEEPNSEY DLWMSTGRVL 

       730        740        750        760        770        780 
EHWHTGSMTA RVPELYRAYP DAQIFMHPED AKARGLQRGD EVLVASPRGE IKTRVETKGR 

       790        800        810        820 
NKPPRGVVFM PFFDARQLVN KLILDATDPL SKETDFKKCP VKVMKA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN53924.1.
RefSeqNP_716479.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8EIJ1.
SMRQ8EIJ1. Positions 38-825.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_0848.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN53924; AAN53924; SO_0848.
GeneID1168695.
KEGGson:SO_0848.
PATRIC23521357. VBISheOne101494_0812.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMARRLSHKD.
OrthoDBEOG6CVV7G.
PhylomeDBQ8EIJ1.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SHEON
AccessionPrimary (citable) accession number: Q8EIJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families