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Q8EIJ1

- NAPA_SHEON

UniProt

Q8EIJ1 - NAPA_SHEON

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi45 – 451Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi52 – 521Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi80 – 801Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:SO_0848
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Tat-type signalUniRule annotationAdd
BLAST
Chaini33 – 826794Periplasmic nitrate reductasePRO_0000046003Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_0848.

Structurei

3D structure databases

ProteinModelPortaliQ8EIJ1.
SMRiQ8EIJ1. Positions 38-825.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 94574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiRRLSHKD.
OrthoDBiEOG6CVV7G.
PhylomeDBiQ8EIJ1.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8EIJ1-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSISRREFLK ANAAVAAATA VGVTLPVKMV EAAESDNIKW DKAPCRFCGV
60 70 80 90 100
GCSVLVGTKA GKVVATKGDP ESPVNRGLNC IKGYFLSKIM YGKDRLTTPL
110 120 130 140 150
LRMKDGKYHK EGEFTPVSWD VAFDTMAAKW KHSIATKGPT SVGMFGSGQW
160 170 180 190 200
TIWEGYAASK LHKAGFLTNN IDPNARHCMA SAVVGFMRTF GIDEPMGCYD
210 220 230 240 250
DLEAADHFVL WGANMAEMHP ILWARLSDRR LSSKDCRVHV LSTFENRSFD
260 270 280 290 300
LADNPMVFHP QSDLVILNYI ANYIIQNKAV NTDFVTKHTK FALGVDDIGY
310 320 330 340 350
GLRPDHPLEK KAKNPGNGKS TPISFDEYAK FVSTYTLEYA AKMSGVEPEK
360 370 380 390 400
LETLAKAYAD PKAKVMSLWT MGINQHVRGV WANNMLYNIH LLTGKIATPG
410 420 430 440 450
NSPFSLTGQP SACGTAREVG TFAHRLPADM VVDNDKHRAI TEKMWQVPEG
460 470 480 490 500
TIPPKPGYHA VLQSRMLKDG KLNCYWTMCT NNMQAGPNIN EEMYPGFRNP
510 520 530 540 550
ENFIVVSDPY PTVTAMAADL ILPTAMWVEK EGAYGNAERR THMWHQQVKA
560 570 580 590 600
PEGAKSDLWQ LVEFSKRFKV AEVWPAELIA KKPEYADKTL YEVLFANGVI
610 620 630 640 650
NKFPTTDCKG DLNDESEHFS FYVQKGIFEE YAAFGRGHGH DLAEFDRYHE
660 670 680 690 700
TRGLRWPVVN DKETLRRFVE GSDPYVKAGE GYNFYGKPDG KAVIFALPYE
710 720 730 740 750
PAAEEPNSEY DLWMSTGRVL EHWHTGSMTA RVPELYRAYP DAQIFMHPED
760 770 780 790 800
AKARGLQRGD EVLVASPRGE IKTRVETKGR NKPPRGVVFM PFFDARQLVN
810 820
KLILDATDPL SKETDFKKCP VKVMKA
Length:826
Mass (Da):92,475
Last modified:March 1, 2003 - v1
Checksum:i33474D23868F6316
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA. Translation: AAN53924.1.
RefSeqiNP_716479.1. NC_004347.2.
WP_011071138.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN53924; AAN53924; SO_0848.
GeneIDi1168695.
KEGGison:SO_0848.
PATRICi23521357. VBISheOne101494_0812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA. Translation: AAN53924.1 .
RefSeqi NP_716479.1. NC_004347.2.
WP_011071138.1. NC_004347.2.

3D structure databases

ProteinModelPortali Q8EIJ1.
SMRi Q8EIJ1. Positions 38-825.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211586.SO_0848.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN53924 ; AAN53924 ; SO_0848 .
GeneIDi 1168695.
KEGGi son:SO_0848.
PATRICi 23521357. VBISheOne101494_0812.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi RRLSHKD.
OrthoDBi EOG6CVV7G.
PhylomeDBi Q8EIJ1.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiNAPA_SHEON
AccessioniPrimary (citable) accession number: Q8EIJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3