Reviewed, UniProtKB/Swiss-Prot Q8EI85 (AMPA1_SHEON)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase 1
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase 1
      Short name=LAP 1
    Leucyl aminopeptidase 1

Gene names

Name:	pepA1
Synonyms:	pepA-1
Ordered Locus Names:	SO_0959

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase 1 HAMAP MF_00181		PRO_0000165796
Sites
	Active site	275	1	Potential
	Active site	350	1	Potential
	Metal binding	263	1	Manganese 2 By similarity
	Metal binding	268	1	Manganese 1 By similarity
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	287	1	Manganese 2 By similarity
	Metal binding	346	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EI85-1 [UniParc]. Last modified March 25, 2003. Version 2. Checksum: 070041251E1BB338	FASTA	500	53,027
	10 20 30 40 50 60 MALACLNSLN ANAEIFSFDT RNSLNSDTLV LFHSADSTTY SLDFLPQSTQ DQLNLAVADN 70 80 90 100 110 120 SFSGKRGEVL EILVPSEIDA KRVLLVGIGD AKTLTPGEIN ALGGNIAAKL ETVPQATVRV 130 140 150 160 170 180 LTQGLNNAPL FGSELAHGIE LRSYRYTQFK ASNRVEKNYQ IGVDDLSLNQ KHHKNLQAVE 190 200 210 220 230 240 AGVFLARDLT NAPAGNMYPE SFANEARKLK SLGVKVTVLE AKDIERLNLG ALAAVGKGSE 250 260 270 280 290 300 RPPKLVVAHW PGSKEAPIAL VGKGITFDSG GYNIKATGTS IARMKSDMAG AATVLGTVKA 310 320 330 340 350 360 MAIQKAPVNL VAIMPMAENM VSGHAMIPGD VITTAQGLTV EVLNTDAEGR LVLADGLWYA 370 380 390 400 410 420 RENYRPSVII DVATLTGSKV SALGTVYAGL FTDSEPLVQQ LTFAGQQVGE KVWRLPLDQA 430 440 450 460 470 480 YDDELKSTIA DLKNTGKEGS AGASAAAMFL KRFAGDQPWA HLDIAGHALT ATDTAVVPAG 490 500 ATGYGVRLLS TWLTQPKAQN

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN54033.1. Different initiation.
RefSeq	NP_716588.1.
3D structure databases
HSSP	HSSP built from PDB template 1LAP based on UniProtKB P00727.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	1168804.
GenomeReviews	Gene locus SO_0959 in contig AE014299_GR.
KEGG	son:SO_0959.
NMPDR	fig|211586.1.peg.895.
TIGR	SO_0959.
Phylogenomic databases
HOGENOM	Q8EI85.
OMA	ECEDLGM.
Enzyme and pathway databases
BioCyc	SONE211586:SO_0959-MON.
BRENDA	3.4.11.1. 257422.
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA1_SHEON

Accession

Primary (citable) accession number: Q8EI85

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 25, 2003
Last modified:	November 3, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)