Q8EI67 (RLMC_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmC
EC=2.1.1.189

Alternative name(s):
23S rRNA(m5U747)-methyltransferase

Gene names

Name:	rlmC
Synonyms:	rumB
Ordered Locus Names:	SO_0980

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	386 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA By similarity. HAMAP-Rule MF_01012
Catalytic activity	S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA. HAMAP-Rule MF_01012
Sequence similarities	Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.
Sequence caution	The sequence AAN54054.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: HAMAP rRNA (uridine-C5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 386	386	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC HAMAP-Rule MF_01012		PRO_0000161936
Sites
	Active site	343	1	Nucleophile By similarity
	Metal binding	7	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	15	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	18	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	94	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	219	1	S-adenosyl-L-methionine By similarity
	Binding site	248	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	269	1	S-adenosyl-L-methionine By similarity
	Binding site	316	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EI67 [UniParc]. Last modified April 3, 2007. Version 2. Checksum: C4B5C92D7421C64C	FASTA	386	43,033
	10 20 30 40 50 60 MSTLVQCGYF ERGQCQSCRH IKLPMVQQLA AKTQELQQLL APFVVSTNTQ FLPPVVGEDS 70 80 90 100 110 120 GFRNKAKMVV LGAAHAPVLG IVSPNGEAVS LCDCLLYPAD MQALLYRLER FVQQAGIPPY 130 140 150 160 170 180 RIDKAKGELK YILLTRSQVR GEYMLRFVLR SPDAIARIER ELPALMAEYP QINVVSVNIQ 190 200 210 220 230 240 PIHMAILEGD EEIFLTENTR LEERFNDVPL FIRPKSFFQT NPHVAAKLYQ TAREWVGEFA 250 260 270 280 290 300 PKSLWDLFCG VGGFGLHCAS KDIPLTGIEI EAEAIACAKM SAQMMGLDNV QFMALDSTDF 310 320 330 340 350 360 AKGDAAATKP DLIIVNPPRR GIGESLCDAL SEFAPRAILY SSCNPKTLAK DLVHIRGYQL 370 380 TRVQLFDLFP HSDHFEVLAM LVKDSR

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54054.1. Different initiation.
RefSeq	NP_716609.2. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EI67.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_0980.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN54054; AAN54054; SO_0980.
GeneID	1168823.
KEGG	son:SO_0980.
PATRIC	23521611. VBISheOne101494_0939.
Phylogenomic databases
eggNOG	COG2265.
HOGENOM	HOG000218547.
KO	K03212.
OMA	QPQHAAI.
ProtClustDB	PRK03522.
Family and domain databases
HAMAP	MF_01012. 23SrRNA_methyltr_RlmC.
InterPro	IPR001566. 23S_rRNA_MeTrfase_RlmD. IPR011825. 23SrRNA_MeTrfase_RlmC. IPR010280. U5_MeTrfase. [Graphical view]
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
TIGRFAMs	TIGR02085. meth_trns_rumB. 1 hit. TIGR00479. rumA. 1 hit.
PROSITE	PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMC_SHEON

Accession

Primary (citable) accession number: Q8EI67

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	April 3, 2007
Last modified:	May 1, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program