Q8EHZ2 (DEF2_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase 2
Short name=PDF 2
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	SO_1062

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 181	181	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082835
Sites
	Active site	152	1	By similarity
	Metal binding	109	1	Iron By similarity
	Metal binding	151	1	Iron By similarity
	Metal binding	155	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EHZ2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BF5C27BADEA587C2	FASTA	181	20,325
	10 20 30 40 50 60 MFKDEMRQTP NKPLPIAVVG EAILKQQAIE VRDFDDTLSQ LASQMAASMV EAKGVGIAAP 70 80 90 100 110 120 QVHSPLALFI MASRPNERYP DAPLMEPLVV VNPQIVLRSL QLEKGEEGCL SVPGQRFTIW 130 140 150 160 170 180 RPQTIVVRYQ NLAGQWQHSE LTGFIARIFQ HEFDHLQGIT LLERSQMPEQ KLMAQEGKPQ A

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54134.1.
RefSeq	NP_716689.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8EHZ2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1168899.
GenomeReviews	Gene locus SO_1062 in contig AE014299_GR.
KEGG	son:SO_1062.
NMPDR	fig|211586.1.peg.988.
PATRIC	23521771. VBISheOne101494_1019.
TIGR	SO_1062.
Phylogenomic databases
HOGENOM	HBG665227.
OMA	YISKRII.
ProtClustDB	CLSK906069.
Enzyme and pathway databases
BioCyc	SONE211586:SO_1062-MONOMER.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
KO	K01462.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. Pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_SHEON

Accession

Primary (citable) accession number: Q8EHZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program