Reviewed, UniProtKB/Swiss-Prot Q8EHW5 (RLMG_SHEON)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	SO_1097

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366512

Sequences

	Sequence		Length	Mass (Da)
	Q8EHW5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A07469EA10F0C81A	FASTA	377	41,783
	10 20 30 40 50 60 MTTQFSVAGI ELELFRYPAS QESNLQAWDA ADEHLINSLI EGGQIAVPTA IINDSFGALS 70 80 90 100 110 120 CALSRINPNW PLRVETDART SFLGTEQNHH RNQLPMDNLQ WFTSRDTLPK DVALVLMKLP 130 140 150 160 170 180 KNLTYFAHQL MRLSQILPAG CRVLVGAKAK SINSSLLALF AKHLGPANAS LAWKKTRVIT 190 200 210 220 230 240 CISDGKPRAL PNGITWNIPE FNLTISNLSN VFAANKLDIG ARIMLDNLPQ GKFNTVVDLG 250 260 270 280 290 300 CGNGVLGLRA AQLYPNADIH FIDDSEMAVA SAKANWTMNQ LAEGKGHFHW DDCMTHLPED 310 320 330 340 350 360 IEPDLVLCNP PFHQGEAITD HIAWQMFLDA RRRLKNGGIL HIVGNRHLAY HVKLQRLFKN 370 CTTVASNGKF VILQAQK

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN54168.1.
RefSeq	NP_716723.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1168930.
GenomeReviews	Gene locus SO_1097 in contig AE014299_GR.
KEGG	son:SO_1097.
NMPDR	fig|211586.1.peg.1018.
TIGR	SO_1097.
Phylogenomic databases
HOGENOM	Q8EHW5.
OMA	NRHLGYH.
Enzyme and pathway databases
BioCyc	SONE211586:SO_1097-MON.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHEON

Accession

Primary (citable) accession number: Q8EHW5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)