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Q8EHP4 (SYL_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SO_1174
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152079

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EHP4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: C78D6209DFB6CA17

FASTA85996,827
        10         20         30         40         50         60 
MQEQYNPSEI EALVQKHWHD TKTFEVTEDQ NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVARFQRLQG KNVLQPIGWD SFGLPAENAA INNKTAPAPW TYQNIEYMKN QLKLLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TKLYEKGLVY KKTASVNWCP NDETVLANEQ VQDGCCWRCD 

       190        200        210        220        230        240 
TPVEQKEIPQ WFIKITAYAE ELLNDIDTLD GWPEQVKTMQ RNWIGRSEGV EMTFGVAGSD 

       250        260        270        280        290        300 
KSFDIYTTRP DTLMGVTYVA IAAGHPLAEI AAQTNPELAA FIDECKNSTT SEAELATMEK 

       310        320        330        340        350        360 
RGVATGLYAI HPITGKQVPI WAANFVLMNY GTGAVMSVPG HDQRDYEFAK KYHLPIEAVI 

       370        380        390        400        410        420 
KPAEGDLDIS EAAYTEKGIL FNSGEFDGLD FDGAFNVIAN KLVAEGKGKR QVNYRLRDWG 

       430        440        450        460        470        480 
VSRQRYWGAP IPMVTLADGT VIPTPEDQLP VILPEDVVMD GIQSPIKADK EWAKTQVNGQ 

       490        500        510        520        530        540 
DALRETDTFD TFMESSWYYA RYCSPQAEQM LDPTKANYWL PVDQYIGGIE HACMHLLYFR 

       550        560        570        580        590        600 
FFHKLLRDAG LVNTNEPAKQ LLTQGMVLAD AFYYTNDKGA RVWVSPLDVA TTEKDDKGRI 

       610        620        630        640        650        660 
TKAIDKDGNE LVYTGMCKMS KSKNNGIDPQ VMVEKYGADT VRLFMMFASP PELTLEWQES 

       670        680        690        700        710        720 
GVEGAHRFIK RLWKLASEYV AQDNSEALDV SKLTSEQKAL RREVHKTIAK VTDDIGRRQM 

       730        740        750        760        770        780 
FNTAVAAVME LMNHLQKAPQ TTGQDRAIIG EALSAVVRLL YPIIPHVSFT LWNELGNTNS 

       790        800        810        820        830        840 
IEDSQWPVVD ESALVEDSKL IVVQVNGKVR AKITVAADAD QASVEALGMA DEQVIKYLDG 

       850 
VTVRKVIYVP GKLLSIVAN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN54244.1.
RefSeqNP_716799.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8EHP4.
SMRQ8EHP4. Positions 228-412.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_1174.

Proteomic databases

PRIDEQ8EHP4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN54244; AAN54244; SO_1174.
GeneID1169003.
KEGGson:SO_1174.
PATRIC23521988. VBISheOne101494_1126.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHEON
AccessionPrimary (citable) accession number: Q8EHP4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries