ID   TORA_SHEON              Reviewed;         829 AA.
AC   Q8EHI9;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 45.
DE   RecName: Full=Trimethylamine-N-oxide reductase;
DE            Short=TMAO reductase;
DE            Short=Trimethylamine oxidase;
DE            EC=1.7.2.3;
DE   Flags: Precursor;
GN   Name=torA; OrderedLocusNames=SO_1232;
OS   Shewanella oneidensis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=70863;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   MEDLINE=22297686; PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A.,
RA   Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J.,
RA   Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S.,
RA   Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A.,
RA   White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M.,
RA   Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J.,
RA   Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O.,
RA   Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- FUNCTION: Reduces trimethylamine-N-oxide (TMAO) into
CC       trimethylamine; an anaerobic reaction coupled to energy-yielding
CC       reactions (By similarity).
CC   -!- CATALYTIC ACTIVITY: Trimethylamine + 2 (ferricytochrome c)-subunit
CC       + H(2)O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit +
CC       2 H(+).
CC   -!- COFACTOR: Molybdenum (molybdopterin) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Periplasm (By similarity).
CC   -!- PTM: Predicted to be exported by the Tat system. The position of
CC       the signal peptide cleavage has not been experimentally proven.
CC   -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC       oxidoreductase family.
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DR   EMBL; AE014299; AAN54300.1; -; Genomic_DNA.
DR   RefSeq; NP_716855.1; -.
DR   HSSP; O87948; 1TMO.
DR   GeneID; 1169058; -.
DR   GenomeReviews; AE014299_GR; SO_1232.
DR   KEGG; son:SO_1232; -.
DR   NMPDR; fig|211586.1.peg.1143; -.
DR   TIGR; SO_1232; -.
DR   HOGENOM; Q8EHI9; -.
DR   OMA; Q8EHI9; NAETHES.
DR   BioCyc; SONE211586:SO_1232-MON; -.
DR   BRENDA; 1.7.2.3; 257422.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0030151; F:molybdenum ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050626; F:trimethylamine-N-oxide reductase (cytochrom...; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR009010; Asp_de-COase-like_fold.
DR   InterPro; IPR006656; Mopterin_OxRdtase.
DR   InterPro; IPR006655; Mopterin_OxRdtase_prok_CS.
DR   InterPro; IPR006657; MPT_dinuc_bd.
DR   InterPro; IPR006311; Tat.
DR   InterPro; IPR011887; TorA.
DR   InterPro; IPR017909; Twin_arg_translocation_Tat.
DR   Gene3D; G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.
DR   Pfam; PF00384; Molybdopterin; 1.
DR   Pfam; PF01568; Molydop_binding; 1.
DR   TIGRFAMs; TIGR01409; TAT_signal_seq; 1.
DR   TIGRFAMs; TIGR02164; torA; 1.
DR   PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; FALSE_NEG.
DR   PROSITE; PS00490; MOLYBDOPTERIN_PROK_2; 1.
DR   PROSITE; PS00932; MOLYBDOPTERIN_PROK_3; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Molybdenum; Oxidoreductase; Periplasm; Signal.
FT   SIGNAL        1     31       Tat-type signal (Potential).
FT   CHAIN        32    829       Trimethylamine-N-oxide reductase.
FT                                /FTId=PRO_0000019158.
SQ   SEQUENCE   829 AA;  92594 MW;  9F065DFE90E05808 CRC64;
     MNRRDFLKGL ASTSFVALGG SSVLAPLNAL ANTGLNENEW LTTGSHFGAF KIKRKNGMIA
     EVKAFDLDKY PTDMINGIRG MVYNPSRVRY PMVRLDFLLK GHKSNTQQRG DFRFVRVTWD
     KALKLFKHSL DEVQTKYGPS GLHAGQTGWR ATGQLHSSTS HMQRAVGMHG NFVKKIGDYS
     TGAGQTILPY VLGSTEVYAQ GTSWPLILEN SNTIVLWSND PYKNLQVGWN AETHEAFAYL
     AQLKEKVKQG KIRVISIDPV VTKTQAYLGC EQLYVNPQTD VTLMLAIAHE MITQKLHDEK
     FIQGYSLGFE EFVPYVMGTK DGIAKTPEWA APICGVEPHI IRDLAKTLVK GRTQIMMGWC
     IQRQQHGEQP YWMAAVLATM IGQIGLPGGG ISYGHHYSSI GVPATTAAAP GAFPRNLDEN
     QKPLFDSTDF KGASSTIPVA RWIDAILEPG KTIDANGSKV VYPDIKMMIF SGNNPWNHHQ
     DRNRMKQAFQ KLECVVSIDV NWTATCRFSD IVLPACTTYE RNDIDVYGAY ANRGILAMQK
     MVEPLFESLS DFEIFTRFAA LLGKEKEYTR NMSEMEWIET LYNECKAANA GKYEMPDFAT
     FWKQGYVHFG EGELWTRHAD FRNDPEINPL GTPSGLIEIF SRKIEQFGYD DCQGHPMWME
     KAERSHGGPG SNKYPMWLQS CHPDHRLHSQ MCESKEYRET YTVNGREPVY ISPEDAKTRG
     IKDGDIVRVF NDRGQLLAGA VVSDRFPKGV VRIHEGAWYG PVGKDGSVEG GAEIGALCSY
     GDPNTLTLDI GTSKLAQACS AYTCLVEFEK YQGKAPKVSS FDGPIEVEI
//