Reviewed, UniProtKB/Swiss-Prot Q8EHI9 (TORA_SHEON)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torA
Ordered Locus Names:	SO_1232

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 829	798	Trimethylamine-N-oxide reductase		PRO_0000019158

Sequences

	Sequence		Length	Mass (Da)
	Q8EHI9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9F065DFE90E05808	FASTA	829	92,594
	10 20 30 40 50 60 MNRRDFLKGL ASTSFVALGG SSVLAPLNAL ANTGLNENEW LTTGSHFGAF KIKRKNGMIA 70 80 90 100 110 120 EVKAFDLDKY PTDMINGIRG MVYNPSRVRY PMVRLDFLLK GHKSNTQQRG DFRFVRVTWD 130 140 150 160 170 180 KALKLFKHSL DEVQTKYGPS GLHAGQTGWR ATGQLHSSTS HMQRAVGMHG NFVKKIGDYS 190 200 210 220 230 240 TGAGQTILPY VLGSTEVYAQ GTSWPLILEN SNTIVLWSND PYKNLQVGWN AETHEAFAYL 250 260 270 280 290 300 AQLKEKVKQG KIRVISIDPV VTKTQAYLGC EQLYVNPQTD VTLMLAIAHE MITQKLHDEK 310 320 330 340 350 360 FIQGYSLGFE EFVPYVMGTK DGIAKTPEWA APICGVEPHI IRDLAKTLVK GRTQIMMGWC 370 380 390 400 410 420 IQRQQHGEQP YWMAAVLATM IGQIGLPGGG ISYGHHYSSI GVPATTAAAP GAFPRNLDEN 430 440 450 460 470 480 QKPLFDSTDF KGASSTIPVA RWIDAILEPG KTIDANGSKV VYPDIKMMIF SGNNPWNHHQ 490 500 510 520 530 540 DRNRMKQAFQ KLECVVSIDV NWTATCRFSD IVLPACTTYE RNDIDVYGAY ANRGILAMQK 550 560 570 580 590 600 MVEPLFESLS DFEIFTRFAA LLGKEKEYTR NMSEMEWIET LYNECKAANA GKYEMPDFAT 610 620 630 640 650 660 FWKQGYVHFG EGELWTRHAD FRNDPEINPL GTPSGLIEIF SRKIEQFGYD DCQGHPMWME 670 680 690 700 710 720 KAERSHGGPG SNKYPMWLQS CHPDHRLHSQ MCESKEYRET YTVNGREPVY ISPEDAKTRG 730 740 750 760 770 780 IKDGDIVRVF NDRGQLLAGA VVSDRFPKGV VRIHEGAWYG PVGKDGSVEG GAEIGALCSY 790 800 810 820 GDPNTLTLDI GTSKLAQACS AYTCLVEFEK YQGKAPKVSS FDGPIEVEI

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54300.1.
RefSeq	NP_716855.1.
3D structure databases
SMR	Q8EHI9. Positions 36-829.
ModBase	Search...
Genome annotation databases
GeneID	1169058.
GenomeReviews	Gene locus SO_1232 in contig AE014299_GR.
KEGG	son:SO_1232.
NMPDR	fig|211586.1.peg.1143.
TIGR	SO_1232.
Phylogenomic databases
HOGENOM	HBG304064.
OMA	NAETHES.
Enzyme and pathway databases
BioCyc	SONE211586:SO_1232-MONOMER.
BRENDA	1.7.2.3. 257422.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. IPR011887. TorA. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit. TIGR02164. torA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORA_SHEON

Accession

Primary (citable) accession number: Q8EHI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)