Q8EH68 (TYRA_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-protein

Including the following 2 domains:

1. Chorismate mutase
   Short name=CM
   EC=5.4.99.5
2. Prephenate dehydrogenase
   Short name=PDH
   EC=1.3.1.12

Gene names

Name:	tyrA
Ordered Locus Names:	SO_1362

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate = prephenate. Prephenate + NAD+ = 4-hydroxyphenylpyruvate + CO2 + NADH.
Pathway	Amino-acid biosynthesis; L-tyrosine biosynthesis; (4-hydroxyphenyl)pyruvate from prephenate (NAD(+) route): step 1/1. Metabolic intermediate biosynthesis; prephenate biosynthesis; prephenate from chorismate: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 chorismate mutase domain. Contains 1 prephenate/arogenate dehydrogenase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tyrosine biosynthesis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	chorismate metabolic process Inferred from electronic annotation. Source: InterPro tyrosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chorismate mutase activity Inferred from electronic annotation. Source: EC prephenate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro prephenate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	T-protein		PRO_0000398177
Regions
	Domain	3 – 94	92	Chorismate mutase
	Domain	103 – 366	264	Prephenate/arogenate dehydrogenase

Sequences

	Sequence		Length	Mass (Da)
	Q8EH68 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F999D5BDAFD50AB7	FASTA	379	42,876
	10 20 30 40 50 60 MNEKTTSELE HLRGLIDGVD QQLLHLLRKR LDLVAQVGTV KHAAGLPIYA PQREAAMLAK 70 80 90 100 110 120 RREEAQTMGI APQLIEDILR RLMRESYLNE KDVGFKQVKN DLGSVVIVGG KGQLGGLFQQ 130 140 150 160 170 180 MLRLSGYQVK VLDKDDWQQA ECLFADAGLV LVTVPIAITC DIIREKLTQL PRDCILADLT 190 200 210 220 230 240 SIKTEPMQAM LAAHKGPVVG FHPMFGPDVG SLAKQVVVVC HGREADKYQW LLEQIAIWGA 250 260 270 280 290 300 RIVEAEPECH DNAMQLVQAM RHFSTFVYGL NLCKEEADIE TLLKFSSPIY RLELAMVGRL 310 320 330 340 350 360 FAQSPELYAD IIFAQQESQH AIGDYLDNYR EALELLKRGD REEFINQFQM VAKWFGDFAP 370 QFQRESRMML QSVSDMKTN

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54427.1.
RefSeq	NP_716982.1. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EH68.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_1362.
Protocols and materials databases
DNASU	1169183.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN54427; AAN54427; SO_1362.
GeneID	1169183.
KEGG	son:SO_1362.
PATRIC	23522378. VBISheOne101494_1311.
Phylogenomic databases
eggNOG	COG0287.
HOGENOM	HOG000275725.
KO	K14187.
OMA	QRFGEAI.
ProtClustDB	PRK11199.
Enzyme and pathway databases
UniPathway	UPA00120; UER00203. UPA00122; UER00961.
Family and domain databases
Gene3D	1.20.59.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR008244. Chor_mut/prephenate_DH_T. IPR002701. Chorismate_mutase. IPR020822. Chorismate_mutase_type_II. IPR011277. CM_T. IPR016040. NAD(P)-bd_dom. IPR003099. Prephen_DH. [Graphical view]
Pfam	PF01817. CM_2. 1 hit. PF02153. PDH. 1 hit. [Graphical view]
PIRSF	PIRSF001499. Chor_mut_pdh_Tpr. 1 hit.
SMART	SM00830. CM_2. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit. SSF48600. Chorismate_mut. 1 hit.
TIGRFAMs	TIGR01799. CM_T. 1 hit.
PROSITE	PS51168. CHORISMATE_MUT_2. 1 hit. PS51176. PDH_ADH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYRA_SHEON

Accession

Primary (citable) accession number: Q8EH68

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program