Q8EH62 (AMPA2_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase 2
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase 2
Short name=LAP 2
EC=3.4.11.10
Leucyl aminopeptidase 2

Gene names

Name:	pepA2
Synonyms:	pepA-2
Ordered Locus Names:	SO_1368

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Probable cytosol aminopeptidase 2 HAMAP MF_00181		PRO_0000165797
Sites
	Active site	281	1	Potential
	Active site	355	1	Potential
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 1 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	292	1	Manganese 2 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EH62 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CD7879C50F0C2FB1	FASTA	502	54,723
	10 20 30 40 50 60 MEFSVKSGSP EKQRSACIVV GVYEPRRLSG IAEQLDKISE GYISNLLRRG DLEGKPGQML 70 80 90 100 110 120 LLHHVPNVLS ERVLLVGCGK ERELDERQYK QIITKTINTL NETGSMEAVC FLTELHVKGR 130 140 150 160 170 180 DTYWKVRQAV ETTNSSLYCF DALKTRKGET RRPLRKLVFN VPTRRELTLG ERAIEHGMAV 190 200 210 220 230 240 SSGMHLCRDV ANMPPNICNP AYLASQARQL AEIHENLHVS TVGEEQMAKL GMNSYLAVGR 250 260 270 280 290 300 ASANESIMTV MEYKGAVDST EKPIVLIGKG LTFDSGGISL KPGEAMDEMK YDMGGAAGVI 310 320 330 340 350 360 GTMKAICEMK LPINVVGILA GCENMPSGNA YRPGDILTTL SGQTVEVLNT DAEGRLVLCD 370 380 390 400 410 420 VLTYVERFDP ELVIDTATLT GACVIALGKH ASGLFSSHNP LAHELLNAGE QSGDRAWRMP 430 440 450 460 470 480 LWDEYQDMLD SPFADMTNLG GRPAGAITAA CFLSRFAKKY NWAHLDVAGT AWNSGANKGS 490 500 TGRPVPILTQ FLINRAGVEL GE

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54433.1.
RefSeq	NP_716988.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8EH62.
SMR	Q8EH62. Positions 1-497.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1169189.
GenomeReviews	Gene locus SO_1368 in contig AE014299_GR.
KEGG	son:SO_1368.
NMPDR	fig|211586.1.peg.1271.
PATRIC	23522390. VBISheOne101494_1317.
TIGR	SO_1368.
Phylogenomic databases
HOGENOM	HBG742580.
OMA	NSGLMST.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	SONE211586:SO_1368-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA2_SHEON

Accession

Primary (citable) accession number: Q8EH62

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program