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Q8EGU7 (GLGB_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:SO_1494
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length745 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7457451,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188742

Sites

Active site4151Nucleophile By similarity
Active site4681Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EGU7 [UniParc].

Last modified May 29, 2013. Version 2.
Checksum: 6194EA3E56302335

FASTA74585,188
        10         20         30         40         50         60 
MTQAQTYFYD GSDVALLNGQ YTDVFSLLGM HSINEGKALV VRCFLRNAQK VDVISLKDGR 

        70         80         90        100        110        120 
KVASLERVNE AGLFAGTLGR RIKPFLYALR VTYPLCELDI IDPYQFGSLL NREDLYLFGE 

       130        140        150        160        170        180 
GSSEQAYRFL GANWRQVDNV EGVHFCVWAP NAKRVSVVGD FNHWDDTRHV MRQHVANGLW 

       190        200        210        220        230        240 
EIFLPGVVEG SHYKFDLVYQ NGERHAKSDP MATQMECAPH NASIVPKKQQ HQWADTQWMD 

       250        260        270        280        290        300 
KRAATAWHRA PMSIYEVQLG SWRRKGEFGE QYFDYQDLIE QLIPYVKEQG FTHIELMPVS 

       310        320        330        340        350        360 
EYPFDGSWGY QPVGLYAPTY RFGDANGFKA FIDACHQAEI GVLLDWVAAH FPKDPHGLVR 

       370        380        390        400        410        420 
FDGTCLYEHE DPRKGTHPDW DTLIYNYGRG EVRSFLLSNA CYWLREFHLD GLRLDAVSSM 

       430        440        450        460        470        480 
LYLDYSREPG QWLPNAYGGR ENLEAIDFLQ MLNQRLYQAI PGICMIAEES TAFAGVTKPT 

       490        500        510        520        530        540 
DSSGLGFGFK WNMGWMNDSL SYLGRDPIYR QYHHNQLTFS LMYAYSEQFM LSISHDEVVH 

       550        560        570        580        590        600 
GKGSLLHKIP GDDWQKFATL RAYYGFMWGH PGKKLLFMGS EFAQRDEWDH DHSLDWHLLA 

       610        620        630        640        650        660 
FEPHQGVQRW LRDLNQLYRQ FPALSVLDYE PQGFRWLDCD NGRDSIFSFV RYGEGNDVPL 

       670        680        690        700        710        720 
VFVVNMTPTV HQGFRIGLPQ GGDFCEYLNS DSYLYGGSNQ GNGGKIIAQA LPWQGMASSA 

       730        740 
LITVPPLGCL ILGPATENIL RESAL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN54555.2.
RefSeqNP_717111.2. NC_004347.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_1494.

Proteomic databases

PRIDEQ8EGU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN54555; AAN54555; SO_1494.
GeneID1169305.
KEGGson:SO_1494.
PATRIC23522632. VBISheOne101494_1438.

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_SHEON
AccessionPrimary (citable) accession number: Q8EGU7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: May 29, 2013
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries