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Q8EGU7

- GLGB_SHEON

UniProt

Q8EGU7 - GLGB_SHEON

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene
glgB, SO_1494
Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei415 – 4151Nucleophile By similarity
Active sitei468 – 4681Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
Ordered Locus Names:SO_1494
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7457451,4-alpha-glucan branching enzyme GlgBUniRule annotationPRO_0000188742Add
BLAST

Proteomic databases

PRIDEiQ8EGU7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_1494.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiGELSHAW.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8EGU7-1 [UniParc]FASTAAdd to Basket

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MTQAQTYFYD GSDVALLNGQ YTDVFSLLGM HSINEGKALV VRCFLRNAQK    50
VDVISLKDGR KVASLERVNE AGLFAGTLGR RIKPFLYALR VTYPLCELDI 100
IDPYQFGSLL NREDLYLFGE GSSEQAYRFL GANWRQVDNV EGVHFCVWAP 150
NAKRVSVVGD FNHWDDTRHV MRQHVANGLW EIFLPGVVEG SHYKFDLVYQ 200
NGERHAKSDP MATQMECAPH NASIVPKKQQ HQWADTQWMD KRAATAWHRA 250
PMSIYEVQLG SWRRKGEFGE QYFDYQDLIE QLIPYVKEQG FTHIELMPVS 300
EYPFDGSWGY QPVGLYAPTY RFGDANGFKA FIDACHQAEI GVLLDWVAAH 350
FPKDPHGLVR FDGTCLYEHE DPRKGTHPDW DTLIYNYGRG EVRSFLLSNA 400
CYWLREFHLD GLRLDAVSSM LYLDYSREPG QWLPNAYGGR ENLEAIDFLQ 450
MLNQRLYQAI PGICMIAEES TAFAGVTKPT DSSGLGFGFK WNMGWMNDSL 500
SYLGRDPIYR QYHHNQLTFS LMYAYSEQFM LSISHDEVVH GKGSLLHKIP 550
GDDWQKFATL RAYYGFMWGH PGKKLLFMGS EFAQRDEWDH DHSLDWHLLA 600
FEPHQGVQRW LRDLNQLYRQ FPALSVLDYE PQGFRWLDCD NGRDSIFSFV 650
RYGEGNDVPL VFVVNMTPTV HQGFRIGLPQ GGDFCEYLNS DSYLYGGSNQ 700
GNGGKIIAQA LPWQGMASSA LITVPPLGCL ILGPATENIL RESAL 745
Length:745
Mass (Da):85,188
Last modified:May 29, 2013 - v2
Checksum:i6194EA3E56302335
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN54555.2.
RefSeqiNP_717111.2. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN54555; AAN54555; SO_1494.
GeneIDi1169305.
KEGGison:SO_1494.
PATRICi23522632. VBISheOne101494_1438.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN54555.2 .
RefSeqi NP_717111.2. NC_004347.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211586.SO_1494.

Proteomic databases

PRIDEi Q8EGU7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN54555 ; AAN54555 ; SO_1494 .
GeneIDi 1169305.
KEGGi son:SO_1494.
PATRICi 23522632. VBISheOne101494_1438.

Phylogenomic databases

eggNOGi COG0296.
HOGENOMi HOG000283037.
KOi K00700.
OMAi GELSHAW.
OrthoDBi EOG6JX7GT.

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiGLGB_SHEON
AccessioniPrimary (citable) accession number: Q8EGU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: May 29, 2013
Last modified: July 9, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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