Q8EGH9 (DAPD_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
EC=2.3.1.117

Alternative name(s):
Tetrahydrodipicolinate N-succinyltransferase
Short name=THDP succinyltransferase
Short name=THP succinyltransferase
Short name=Tetrahydropicolinate succinylase

Gene names

Name:	dapD
Ordered Locus Names:	SO_1625

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate. HAMAP-Rule MF_00811
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3. HAMAP-Rule MF_00811
Subunit structure	Homotrimer By similarity. HAMAP-Rule MF_00811
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00811.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP lysine biosynthetic process via diaminopimelate Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase HAMAP-Rule MF_00811		PRO_0000196970
Sites
	Binding site	104	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EGH9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E0360A50220C73B7	FASTA	274	29,873
	10 20 30 40 50 60 MEALRQRIEA AFEARTDITP STVDERVRSD VQHVINMLDK GELRVAEKID GLWHVHQWLK 70 80 90 100 110 120 KAVLLSFRIF DNAVIDGAET KYFDKVPLKF AEYDEARFKA EAIRVVPSAT VRKGSFIGKN 130 140 150 160 170 180 TVLMPSYVNL GAYVDEGTMV DTWATVGSCA QIGKNVHLSG GVGIGGVLEP LQAGPTIIED 190 200 210 220 230 240 NCFIGARSEI VEGVVVEEGS VISMGVYIGQ STRIYDRETG EVHYGRVPAG SVVVSGNLPS 250 260 270 ACGKYSLYAA IIVKKVDAKT RSKVGINELL RIVD

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN54680.1.
RefSeq	NP_717236.1. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EGH9.
SMR	Q8EGH9. Positions 1-274.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_1625.
Proteomic databases
PRIDE	Q8EGH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN54680; AAN54680; SO_1625.
GeneID	1169420.
KEGG	son:SO_1625.
PATRIC	23522884. VBISheOne101494_1564.
Phylogenomic databases
eggNOG	COG2171.
HOGENOM	HOG000003295.
KO	K00674.
OMA	NQWAKKA.
ProtClustDB	PRK11830.
Enzyme and pathway databases
UniPathway	UPA00034; UER00019.
Family and domain databases
Gene3D	1.10.166.10. 1 hit.
HAMAP	MF_00811. DapD.
InterPro	IPR005664. DapD_Trfase_Hexpep_rpt_fam. IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR023180. THP_succinylTrfase_dom1. IPR011004. Trimer_LpxA-like. [Graphical view]
PANTHER	PTHR19136:SF52. PTHR19136:SF52. 1 hit.
Pfam	PF00132. Hexapep. 2 hits. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR00965. dapD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPD_SHEON

Accession

Primary (citable) accession number: Q8EGH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program