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Q8EGH8

- GLND_SHEON

UniProt

Q8EGH8 - GLND_SHEON

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, SO_1626
Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:SO_1626
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192767Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi211586.SO_1626.

Structurei

3D structure databases

ProteinModelPortaliQ8EGH8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini436 – 566131HD
Add
BLAST
Domaini676 – 75681ACT 1
Add
BLAST
Domaini784 – 86178ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 317317UridylyltransferaseUniRule annotation
Add
BLAST
Regioni318 – 675358Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.
PhylomeDBiQ8EGH8.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EGH8-1 [UniParc]FASTAAdd to Basket

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MNTALLAKQS LIEQNQTLLA RFKAKTPIEE LVTQRCQFVD SLVKQAWQQH    50
GLDQYPIALI AVGGYGRGEL HPHSDVDLLF LFEGELSKAA ETTLSEFIAF 100
LWDANLEIGH SVRTLDDTLR LGRDDITIAT NLLEARLLTG PETLFDQLYD 150
AIRQSDFWTS SNFFIAKRDE QTARHAKASA FDLEPNIKSC PGGLRDIQTI 200
AWVAMRHFGA SRLEELVEYE FLEPAELEEL LECQHFLWKL RFALHMAVGR 250
DENRLLFDVQ RQVAELMGYE DATQLAVEQM MKRYYRTVRR VMELNEMLLQ 300
LFKRATLGHT KALEIQAIDD NYQRRGAFIE ALSDDLFDSG EEIVRLFVHI 350
AKNSNIKGIY APTLRSLRRA RRAQLQPLQE NPLCRQAFME ILKHPRGIAA 400
LSLMHKHGVL SAYLPAWRNI EGQMQFDLFH AYTVDEHTHR LLLNIERFSQ 450
PEQKEEFPLG SVLINQLPKK GLLVLGAIFH DIAKGRGGDH SKLGSSDALA 500
FCKLHGLNDH DGRLVAWLVE NHLVMSVTAQ RRDISDPDVV ADFASKVRDA 550
VHLSYLYCLT VADICATNEK TWNNWKGSLL RDLYFSTQRV LARGKEKPVD 600
IRARVREYQA KAKKELLRRG IKEKDLDTLW QRFKADYFLR HQPNQVAWHA 650
EAILKHKQVD EPLVLVSKHT TRGGTELFVY CQDRPKLFAT VMAVLDNKNI 700
NVHDANIMTS KDNYALDTFV ILEQDGEPVS QLSRIQSIRK ALEKALASDN 750
PKLPRFRKLS RKMKPFNVPT QVSFLESNRH GTSMMELIAL DTPGLLAKVG 800
DIFYRCNTTL LSAKITTIGE RAEDFFILQT NDGLQLNETQ EHTLKEALIS 850
ALSAINTEST N 861
Length:861
Mass (Da):98,320
Last modified:March 1, 2003 - v1
Checksum:i178797ACAE4EAE80
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN54681.1.
RefSeqiNP_717237.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN54681; AAN54681; SO_1626.
GeneIDi1169421.
KEGGison:SO_1626.
PATRICi23522886. VBISheOne101494_1565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN54681.1 .
RefSeqi NP_717237.1. NC_004347.2.

3D structure databases

ProteinModelPortali Q8EGH8.
ModBasei Search...

Protein-protein interaction databases

STRINGi 211586.SO_1626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN54681 ; AAN54681 ; SO_1626 .
GeneIDi 1169421.
KEGGi son:SO_1626.
PATRICi 23522886. VBISheOne101494_1565.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.
PhylomeDBi Q8EGH8.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiGLND_SHEON
AccessioniPrimary (citable) accession number: Q8EGH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi