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Q8EGH8

- GLND_SHEON

UniProt

Q8EGH8 - GLND_SHEON

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:SO_1626
    OrganismiShewanella oneidensis (strain MR-1)
    Taxonomic identifieri211586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000008186: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 861861Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192767Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi211586.SO_1626.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8EGH8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini436 – 566131HDUniRule annotationAdd
    BLAST
    Domaini676 – 75681ACT 1UniRule annotationAdd
    BLAST
    Domaini784 – 86178ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 317317UridylyltransferaseAdd
    BLAST
    Regioni318 – 675358Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.
    PhylomeDBiQ8EGH8.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8EGH8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTALLAKQS LIEQNQTLLA RFKAKTPIEE LVTQRCQFVD SLVKQAWQQH    50
    GLDQYPIALI AVGGYGRGEL HPHSDVDLLF LFEGELSKAA ETTLSEFIAF 100
    LWDANLEIGH SVRTLDDTLR LGRDDITIAT NLLEARLLTG PETLFDQLYD 150
    AIRQSDFWTS SNFFIAKRDE QTARHAKASA FDLEPNIKSC PGGLRDIQTI 200
    AWVAMRHFGA SRLEELVEYE FLEPAELEEL LECQHFLWKL RFALHMAVGR 250
    DENRLLFDVQ RQVAELMGYE DATQLAVEQM MKRYYRTVRR VMELNEMLLQ 300
    LFKRATLGHT KALEIQAIDD NYQRRGAFIE ALSDDLFDSG EEIVRLFVHI 350
    AKNSNIKGIY APTLRSLRRA RRAQLQPLQE NPLCRQAFME ILKHPRGIAA 400
    LSLMHKHGVL SAYLPAWRNI EGQMQFDLFH AYTVDEHTHR LLLNIERFSQ 450
    PEQKEEFPLG SVLINQLPKK GLLVLGAIFH DIAKGRGGDH SKLGSSDALA 500
    FCKLHGLNDH DGRLVAWLVE NHLVMSVTAQ RRDISDPDVV ADFASKVRDA 550
    VHLSYLYCLT VADICATNEK TWNNWKGSLL RDLYFSTQRV LARGKEKPVD 600
    IRARVREYQA KAKKELLRRG IKEKDLDTLW QRFKADYFLR HQPNQVAWHA 650
    EAILKHKQVD EPLVLVSKHT TRGGTELFVY CQDRPKLFAT VMAVLDNKNI 700
    NVHDANIMTS KDNYALDTFV ILEQDGEPVS QLSRIQSIRK ALEKALASDN 750
    PKLPRFRKLS RKMKPFNVPT QVSFLESNRH GTSMMELIAL DTPGLLAKVG 800
    DIFYRCNTTL LSAKITTIGE RAEDFFILQT NDGLQLNETQ EHTLKEALIS 850
    ALSAINTEST N 861
    Length:861
    Mass (Da):98,320
    Last modified:March 1, 2003 - v1
    Checksum:i178797ACAE4EAE80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN54681.1.
    RefSeqiNP_717237.1. NC_004347.2.

    Genome annotation databases

    EnsemblBacteriaiAAN54681; AAN54681; SO_1626.
    GeneIDi1169421.
    KEGGison:SO_1626.
    PATRICi23522886. VBISheOne101494_1565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN54681.1 .
    RefSeqi NP_717237.1. NC_004347.2.

    3D structure databases

    ProteinModelPortali Q8EGH8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211586.SO_1626.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN54681 ; AAN54681 ; SO_1626 .
    GeneIDi 1169421.
    KEGGi son:SO_1626.
    PATRICi 23522886. VBISheOne101494_1565.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.
    PhylomeDBi Q8EGH8.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MR-1.

    Entry informationi

    Entry nameiGLND_SHEON
    AccessioniPrimary (citable) accession number: Q8EGH8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3