Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8EEW2 (NAGZ_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:SO_2250
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_0000210798

Regions

Region164 – 1652Substrate binding By similarity

Sites

Active site1771Proton donor/acceptor By similarity
Active site2491Nucleophile By similarity
Binding site611Substrate By similarity
Binding site691Substrate By similarity
Binding site1341Substrate By similarity
Site1751Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8EEW2 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: AB603E9104D16065

FASTA34237,193
        10         20         30         40         50         60 
MSYLMLDLLS LDVSEAEAEM LRHPQVGGLI LFSRNFTSRE QLIALVQQIR QIRPEILIAV 

        70         80         90        100        110        120 
DHEGGRVQRF REGFTLIPAM GDILPAAKGD MALAKRWACE LGFLMAIELL ACDIDLSFAP 

       130        140        150        160        170        180 
VLDLNGISQV IGKRSFSANP DEVIALARSF IEGMAQAGMG AVGKHFPGHG SVAADSHIAQ 

       190        200        210        220        230        240 
PIDEREGEVI FNQDILPFKE LIFNGKLSGI MPAHVIYPKV DPNPAGFSSY WLKQILRKEL 

       250        260        270        280        290        300 
GFNGVIFSDD LGMKGASFAG DYVGRAKAAL DAGCDMILVC NDNPGVMTLL NGFVWPEAAP 

       310        320        330        340 
QHPASLLKPN MAQTALALEN SARWENAKQL AEQIQLAQQA KV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN55290.1.
RefSeqNP_717846.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8EEW2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_2250.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN55290; AAN55290; SO_2250.
GeneID1169981.
KEGGson:SO_2250.
PATRIC23524121. VBISheOne101494_2166.

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMALLNGFVW.
OrthoDBEOG6BCT06.
PhylomeDBQ8EEW2.

Enzyme and pathway databases

UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_SHEON
AccessionPrimary (citable) accession number: Q8EEW2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries