Q8EEN9 (ASTE_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	SO_2338

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000174648
Sites
	Active site	224	1	Potential
	Metal binding	63	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	160	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EEN9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0ECF6CEA903B21F7	FASTA	344	38,783
	10 20 30 40 50 60 MLQALLDSKD FLALTLANPQ TLGDEFSFTL GEHTRVNVWD TGVIVFEPAQ PQGKDVILSC 70 80 90 100 110 120 GVHGNETAPI ELCNTLIKQL LQQKIIAKQR TLFLIGNPLA INNGTRIIDE NMNRLFSGEH 130 140 150 160 170 180 SNPPGLVNPE RLRAKKLETY VDRFFKAAAA GRQRIHYDLH TAMRASKHEK FAIYPYRPGR 190 200 210 220 230 240 AYSAEQIMFL AASGVDTVLF HHEPTTTFSY FSSEQYGADA FTIELGKVYP MGQNDMTRFI 250 260 270 280 290 300 AAQEMFTRLI TDKPLQLESF STDKVNLYQV CRVINKHFDD FEFTFATDVE NFRAFPKGFV 310 320 330 340 LAREGGQEIK VEQEVESIVF PNAKVPIGNR TVICLIPSVA PDVR

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN55372.1.
RefSeq	NP_717928.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8EEN9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1170061.
GenomeReviews	Gene locus SO_2338 in contig AE014299_GR.
KEGG	son:SO_2338.
NMPDR	fig|211586.1.peg.2117.
PATRIC	23524297. VBISheOne101494_2252.
TIGR	SO_2338.
Phylogenomic databases
HOGENOM	HBG298271.
OMA	RWRWLGH.
ProtClustDB	PRK05324.
Enzyme and pathway databases
BioCyc	SONE211586:SO_2338-MONOMER.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
KO	K05526.
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. Arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_SHEON

Accession

Primary (citable) accession number: Q8EEN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program