Q8EEE6 (CMOB_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (mo5U34)-methyltransferase
EC=2.1.1.-

Gene names

Name:	cmoB
Ordered Locus Names:	SO_2436

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA By similarity. HAMAP-Rule MF_01590
Sequence similarities	Belongs to the methyltransferase superfamily. CmoB family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA (uracil) methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	tRNA (mo5U34)-methyltransferase HAMAP-Rule MF_01590		PRO_0000313969

Sequences

	Sequence		Length	Mass (Da)
	Q8EEE6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 37D4442C79FFBE2D	FASTA	330	37,834
	10 20 30 40 50 60 MINFSSFYQQ IADSNLQHWL ETLPAILGKW QREHKHGNLP KWEKVLNKLH YPSPDSVDLV 70 80 90 100 110 120 DSVTIGTGEQ LTPGEKEKLE NLLRLFMPWR KGPFHIHGIH IDTEWRSDWK WDRVKPHISP 130 140 150 160 170 180 LQNRTVLDVG CGSGYHMWRM LGAGAKRVVG IDPSPLFLCQ FEAVKRLSGE NHPVHLLPLG 190 200 210 220 230 240 IEELPPLDAF DTVFSMGVLY HRRSPIDHLL QLRDQLRMGG ELVLETLVID GDENAVLVPQ 250 260 270 280 290 300 DRYGKMNNVW FIPSVAALML WLKKCDFTDI RCVDTDVTAL AEQRRTDWMP NESLVEYLDP 310 320 330 KDITKTVEGY PAPKRATIIA VKNQPNQDLS

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN55470.1.
RefSeq	NP_718026.1. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EEE6.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_2436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN55470; AAN55470; SO_2436.
GeneID	1170149.
KEGG	son:SO_2436.
PATRIC	23524487. VBISheOne101494_2343.
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HOG000218456.
KO	K15257.
OMA	WRKGPFE.
ProtClustDB	PRK15068.
Family and domain databases
HAMAP	MF_01590. tRNA_methyltr_CmoB.
InterPro	IPR010017. tRNA_mo5U34_MeTrfase. [Graphical view]
Pfam	PF08003. Methyltransf_9. 1 hit. [Graphical view]
TIGRFAMs	TIGR00452. TIGR00452. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CMOB_SHEON

Accession

Primary (citable) accession number: Q8EEE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program