Q8EE27 (GLO2_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	SO_2563

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144809
Sites
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 2 By similarity
	Metal binding	60	1	Zinc 2 By similarity
	Metal binding	121	1	Zinc 1 By similarity
	Metal binding	138	1	Zinc 1 By similarity
	Metal binding	138	1	Zinc 2 By similarity
	Metal binding	176	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EE27 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0647CFF156B1B1BC	FASTA	267	29,167
	10 20 30 40 50 60 MLTITAINAF NDNYIWVLQQ DTQQAVYVVD PGDVNVVLDY LNAHQLTLAG ILITHHHRDH 70 80 90 100 110 120 TGGIAALVAY VEQTTGHTLA VYGPQSEAIQ GVNIAIEPQI TQILHLPFLN SPVQVLSVPG 130 140 150 160 170 180 HTAGHIAYLV DGALFCGDTL FSGGCGRLFE GTPAQMCHSL RLLAALPAET RVYCAHEYTL 190 200 210 220 230 240 ANLKFAQAAD PSNAKLKAYN EQATALRAQG KATIPSTIGL ERSINPFLRG LTPTIVDSIK 250 260 QQFCDQDLSN VDELTYFTLL RQWKDIF

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN55593.1.
RefSeq	NP_718149.1. NC_004347.1.
3D structure databases
HSSP	HSSP built from PDB template 1QH5 based on UniProtKB Q16775.
ProteinModelPortal	Q8EE27.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1170268.
GenomeReviews	Gene locus SO_2563 in contig AE014299_GR.
KEGG	son:SO_2563.
NMPDR	fig|211586.1.peg.2322.
PATRIC	23524755. VBISheOne101494_2467.
TIGR	SO_2563.
Phylogenomic databases
HOGENOM	HBG753931.
OMA	ITHHHRD.
ProtClustDB	CLSK906771.
Enzyme and pathway databases
BioCyc	SONE211586:SO_2563-MONOMER.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_SHEON

Accession

Primary (citable) accession number: Q8EE27

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program