Q8EDN7 (ASTB_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-succinylarginine dihydrolase
EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	SO_2706

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity. HAMAP MF_01172
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity. HAMAP MF_01172
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_0000262376
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	250	1	By similarity
	Active site	368	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	214	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity
	Binding site	362	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EDN7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: BD0619B1A831ECCF	FASTA	444	49,061
	10 20 30 40 50 60 MKHFEANFDG LVGPTHNYAG LSFGNVASLN NAALVSNPKA AAKQGLQKAK ALADLGMIQG 70 80 90 100 110 120 MLAPQERPDL NTLRRIGFSG SDAQVLQQAA KTAPALLNAC CSASSMWTAN AATVSPSADT 130 140 150 160 170 180 RDGKLHFTPA NLVDKLHRSI EPITTGRILT ATFNDPHYFY HHNHLPEHNS FGDEGAANHT 190 200 210 220 230 240 RLCQEYGHAG VELFVYGQEA TNPHAPKPLK FPARQTLEAS MAIARLHQLE EDNCVFIQQN 250 260 270 280 290 300 PAVIDQGVFH NDVIAVGNQN VLFYHEQAFL NTQAKLDEIK RKLDTELYFI EVPTAKVSIN 310 320 330 340 350 360 DAVKSYLFNT QIITLPSGEM AIIAPTDCQE NPAVYAYLNE LLSLNTPIKQ VLYFDVKQSM 370 380 390 400 410 420 QNGGGPACLR LRVAMNEREV AAVNQHTLLT DALFTRLNTW VEKHYRDRLS TEDLADPQLV 430 440 IESRTALDEL TQIMKLGSVY PFQR

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN55734.1.
RefSeq	NP_718290.1. NC_004347.1.
3D structure databases
HSSP	HSSP built from PDB template 1YNF based on UniProtKB P76216.
ProteinModelPortal	Q8EDN7.
SMR	Q8EDN7. Positions 3-444.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1170407.
GenomeReviews	Gene locus SO_2706 in contig AE014299_GR.
KEGG	son:SO_2706.
NMPDR	fig|211586.1.peg.2459.
PATRIC	23525035. VBISheOne101494_2607.
TIGR	SO_2706.
Phylogenomic databases
HOGENOM	HBG289862.
OMA	VIAVGNK.
ProtClustDB	PRK13281.
Enzyme and pathway databases
BioCyc	SONE211586:SO_2706-MONOMER.
Family and domain databases
HAMAP	MF_01172. AstB. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
KO	K01484.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. Arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_SHEON

Accession

Primary (citable) accession number: Q8EDN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program