Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Important for catalytic activityUniRule annotation1
Sitei138Important for catalytic activityUniRule annotation1

GO - Molecular functioni

  • 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  • 3-hydroxybutyryl-CoA epimerase activity Source: TIGR
  • enoyl-CoA hydratase activity Source: TIGR
  • NAD binding Source: InterPro

GO - Biological processi

  • fatty acid beta-oxidation Source: UniProtKB-UniPathway
  • fatty acid catabolic process Source: TIGR

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciSONE211586:G1GMP-2859-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:SO_3088
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001093081 – 705Fatty acid oxidation complex subunit alphaAdd BLAST705

Proteomic databases

PaxDbiQ8ECP7

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_3088

Structurei

3D structure databases

SMRiQ8ECP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 188Enoyl-CoA hydrataseUniRule annotationAdd BLAST188
Regioni308 – 7053-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST398

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit

Sequencei

Sequence statusi: Complete.

Q8ECP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKTFNLTRR EDGIAILTMD VPGETMNTLK AEFGPEISEI LSEIKRDSSI
60 70 80 90 100
RGLVLISGKK DSFVAGADIS MLDACQTAGD AKALSQQGHV VFNELEALNI
110 120 130 140 150
PVVAAIHGAC LGGGLELALA CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT
160 170 180 190 200
QRLPRLVGIT TALDMMLTGK QIRPKQALKM GLVNDVVPQT ILLQTAVEMA
210 220 230 240 250
LAGKQIAKPV KKSLVNQLLE GTGFGRNIIF DQAAKQVAKK TQGNYPAPAK
260 270 280 290 300
IIDCVRQGIA KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET
310 320 330 340 350
GAEGATPRKV KKAVILGGGL MGGGIASVTT TKAKIPARVK DINEKGLSNA
360 370 380 390 400
LSYAYKLLDK GVKRRHMTPA ARDNLMALMT TTTEYKGVKD ADIVVEAVFE
410 420 430 440 450
DLALKHQMVK DIERECGEHT IFASNTSSLP IGQIAQAASR PENVIGLHYF
460 470 480 490 500
SPVEKMPLVE VIAHAKTSPE TIATTVAFAR KQGKTPIVVQ DGAGFYVNRI
510 520 530 540 550
LALYMNEAAQ LLLEGQSVEH LDKALVKFGF PVGPITLLDE VGIDVGAKIS
560 570 580 590 600
PILEKELGER FKAPAAFDKL LSDDRKGRKN GKGFYQYGAA SKKKAVDETV
610 620 630 640 650
YGVLGIKPGV DKEMSAVAER CVVQMLNEAV RCLDAGIIAS PRDGDIGAIF
660 670 680 690 700
GIGFPPFLGG PFHYIDTLGA DNLVKILERY QTQYGDRFEP CQRLKAMAAE

KARFF
Length:705
Mass (Da):75,702
Last modified:May 29, 2013 - v2
Checksum:i08CFD86050AF665D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014299 Genomic DNA Translation: AAN56095.2
RefSeqiNP_718651.2, NC_004347.2
WP_011072985.1, NC_004347.2

Genome annotation databases

EnsemblBacteriaiAAN56095; AAN56095; SO_3088
GeneIDi1170773
KEGGison:SO_3088
PATRICifig|211586.12.peg.2984

Similar proteinsi

Entry informationi

Entry nameiFADJ_SHEON
AccessioniPrimary (citable) accession number: Q8ECP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 29, 2013
Last modified: April 25, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health