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Q8ECP7 (FADJ_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fatty acid oxidation complex subunit alpha

Including the following 2 domains:

  1. Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase
    EC=4.2.1.17
    EC=5.1.2.3
  2. 3-hydroxyacyl-CoA dehydrogenase
    EC=1.1.1.35
Gene names
Name:fadJ
Ordered Locus Names:SO_3088
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity. HAMAP-Rule MF_01617

Catalytic activity

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP-Rule MF_01617

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP-Rule MF_01617

(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP-Rule MF_01617

Pathway

Lipid metabolism; fatty acid beta-oxidation. HAMAP-Rule MF_01617

Subunit structure

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01617.

Sequence similarities

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.

In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Fatty acid oxidation complex subunit alpha HAMAP-Rule MF_01617
PRO_0000109308

Regions

Region1 – 188188Enoyl-CoA hydratase By similarity
Region308 – 7053983-hydroxyacyl-CoA dehydrogenase By similarity

Sites

Site1161Important for catalytic activity By similarity
Site1381Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ECP7 [UniParc].

Last modified May 29, 2013. Version 2.
Checksum: 08CFD86050AF665D

FASTA70575,702
        10         20         30         40         50         60 
MGKTFNLTRR EDGIAILTMD VPGETMNTLK AEFGPEISEI LSEIKRDSSI RGLVLISGKK 

        70         80         90        100        110        120 
DSFVAGADIS MLDACQTAGD AKALSQQGHV VFNELEALNI PVVAAIHGAC LGGGLELALA 

       130        140        150        160        170        180 
CHQRVCSDDG KTMLGVPEVQ LGLLPGGGGT QRLPRLVGIT TALDMMLTGK QIRPKQALKM 

       190        200        210        220        230        240 
GLVNDVVPQT ILLQTAVEMA LAGKQIAKPV KKSLVNQLLE GTGFGRNIIF DQAAKQVAKK 

       250        260        270        280        290        300 
TQGNYPAPAK IIDCVRQGIA KGMQKGLEVE ASHFAELVVS KESEALRSIF FATTEMKKET 

       310        320        330        340        350        360 
GAEGATPRKV KKAVILGGGL MGGGIASVTT TKAKIPARVK DINEKGLSNA LSYAYKLLDK 

       370        380        390        400        410        420 
GVKRRHMTPA ARDNLMALMT TTTEYKGVKD ADIVVEAVFE DLALKHQMVK DIERECGEHT 

       430        440        450        460        470        480 
IFASNTSSLP IGQIAQAASR PENVIGLHYF SPVEKMPLVE VIAHAKTSPE TIATTVAFAR 

       490        500        510        520        530        540 
KQGKTPIVVQ DGAGFYVNRI LALYMNEAAQ LLLEGQSVEH LDKALVKFGF PVGPITLLDE 

       550        560        570        580        590        600 
VGIDVGAKIS PILEKELGER FKAPAAFDKL LSDDRKGRKN GKGFYQYGAA SKKKAVDETV 

       610        620        630        640        650        660 
YGVLGIKPGV DKEMSAVAER CVVQMLNEAV RCLDAGIIAS PRDGDIGAIF GIGFPPFLGG 

       670        680        690        700 
PFHYIDTLGA DNLVKILERY QTQYGDRFEP CQRLKAMAAE KARFF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014299 Genomic DNA. Translation: AAN56095.2.
RefSeqNP_718651.2. NC_004347.2.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_3088.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN56095; AAN56095; SO_3088.
GeneID1170773.
KEGGson:SO_3088.
PATRIC23525814. VBISheOne101494_2984.

Phylogenomic databases

eggNOGCOG1250.
HOGENOMHOG000261346.
KOK01782.
OrthoDBEOG6M9F0M.

Enzyme and pathway databases

UniPathwayUPA00659.

Family and domain databases

Gene3D1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPMF_01617. FadJ.
InterProIPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsTIGR02440. FadJ. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFADJ_SHEON
AccessionPrimary (citable) accession number: Q8ECP7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: May 29, 2013
Last modified: July 9, 2014
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways