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Q8ECL7 (GH109_SHEON) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-N-acetylgalactosaminidase

EC=3.2.1.49
Alternative name(s):
Glycosyl hydrolase family 109 protein
Gene names
Name:nagA
Ordered Locus Names:SO_3120
OrganismShewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]
Taxonomic identifier211586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length459 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycosidase that has specific alpha-N-acetylgalactosaminidase activity.

Catalytic activity

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids. Ref.1

Cofactor

Binds 1 NAD+ per subunit. The NAD cannot dissociate By similarity.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Sequence similarities

Belongs to the Gfo/Idh/MocA family. Glycosyl hydrolase 109 subfamily.

Ontologies

Keywords
   DomainSignal
   LigandNAD
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functionalpha-N-acetylgalactosaminidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

oxidoreductase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131Tat-type signal Potential
Chain32 – 459428Alpha-N-acetylgalactosaminidase
PRO_0000348561

Regions

Nucleotide binding64 – 652NAD By similarity
Nucleotide binding135 – 1384NAD By similarity
Nucleotide binding155 – 1562NAD By similarity
Region244 – 2474Substrate binding By similarity

Sites

Binding site861NAD By similarity
Binding site1841NAD By similarity
Binding site2131Substrate By similarity
Binding site2321Substrate By similarity
Binding site2441NAD By similarity
Binding site3261Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8ECL7 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 4567186636917ADF

FASTA45951,965
        10         20         30         40         50         60 
MHNIHRRHFL KAAGAVTAGL VTANIALNAN ASSVAPKPSS GKSVIGLIAP KMEVVRVGFI 

        70         80         90        100        110        120 
GVGERGFSHV EQFCHLEGVE LKAICDTHQA VVDRAVEHIV KQKRPKPAVY TGNDLSYREL 

       130        140        150        160        170        180 
LNRDDIDIVI ISTPWEWHAP MAIDTMESGK HAFVEVPLAL TVEECWQIID TAERTQKNCM 

       190        200        210        220        230        240 
MMENVNYGRE ELMVLNMVRQ GLFGELLHGE AAYIHELRWQ MKEINHKTGS WRTYWHTKRN 

       250        260        270        280        290        300 
GNLYPTHGLG PVSQYMNINR GDRFDYLTSM SSPALGRALY AKREFPADHE RNQLKYINGD 

       310        320        330        340        350        360 
MSTSLIKTVK GRTIMVQHDT TTPRPYSRHN LIQGTNGVFA GFPNRIAVEN DGFGTSYHKW 

       370        380        390        400        410        420 
DTDMQKWYDK YDHPLWQRIG KEAEINGGHG GMDFVMLWRM VYCLRNGEAL DQDVYDGASW 

       430        440        450 
SVVNILSEQS LNNRSNSVNF PDFTRGAWEH AKPLGIVGA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM039445 Genomic DNA. Translation: CAJ01377.1.
AE014299 Genomic DNA. Translation: AAN56125.1.
RefSeqNP_718681.1. NC_004347.2.

3D structure databases

ProteinModelPortalQ8ECL7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING211586.SO_3120.

Protein family/group databases

CAZyGH109. Glycoside Hydrolase Family 109.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN56125; AAN56125; SO_3120.
GeneID1170809.
KEGGson:SO_3120.
PATRIC23525886. VBISheOne101494_3020.

Phylogenomic databases

eggNOGCOG0673.
HOGENOMHOG000252553.
OMAHNIHRRH.
OrthoDBEOG61P6MK.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
TIGRFAMsTIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGH109_SHEON
AccessionPrimary (citable) accession number: Q8ECL7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries