Q8ECL7 (GH109_SHEON) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 65.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-N-acetylgalactosaminidase EC=3.2.1.49 Alternative name(s): Glycosyl hydrolase family 109 protein | ||||
| Gene names |
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| Organism | Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP] | ||||
| Taxonomic identifier | 211586 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella ›  |
Protein attributes
| Sequence length | 459 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Glycosidase that has specific alpha-N-acetylgalactosaminidase activity. |
| Catalytic activity | Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids. Ref.1 |
| Cofactor | Binds 1 NAD+ per subunit. The NAD cannot dissociate By similarity. |
| Post-translational modification | Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. |
| Sequence similarities | Belongs to the Gfo/Idh/MocA family. Glycosyl hydrolase 109 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Domain | Signal |
| Ligand | NAD |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | oxidation-reduction process Inferred from electronic annotation. Source: GOC |
| Molecular_function | alpha-N-acetylgalactosaminidase activity Inferred from electronic annotation. Source: EC nucleotide bindingInferred from electronic annotation. Source: InterPro oxidoreductase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 31 | 31 | Tat-type signal Potential | ||||||
| Chain | 32 – 459 | 428 | Alpha-N-acetylgalactosaminidase | PRO_0000348561 | |||||
Regions | |||||||||
| Nucleotide binding | 64 – 65 | 2 | NAD By similarity | ||||||
| Nucleotide binding | 135 – 138 | 4 | NAD By similarity | ||||||
| Nucleotide binding | 155 – 156 | 2 | NAD By similarity | ||||||
| Region | 244 – 247 | 4 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Binding site | 86 | 1 | NAD By similarity | ||||||
| Binding site | 184 | 1 | NAD By similarity | ||||||
| Binding site | 213 | 1 | Substrate By similarity | ||||||
| Binding site | 232 | 1 | Substrate By similarity | ||||||
| Binding site | 244 | 1 | NAD By similarity | ||||||
| Binding site | 326 | 1 | Substrate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY. Strain: ATCC 70050. |
| [2] | "Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T.  Fraser C.M.Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AM039445 Genomic DNA. Translation: CAJ01377.1. AE014299 Genomic DNA. Translation: AAN56125.1. |
| RefSeq | NP_718681.1. NC_004347.2. |
3D structure databases | |
| ProteinModelPortal | Q8ECL7. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 211586.SO_3120. |
Protein family/group databases | |
| CAZy | GH109. Glycoside Hydrolase Family 109. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAN56125; AAN56125; SO_3120. |
| GeneID | 1170809. |
| KEGG | son:SO_3120. |
| PATRIC | 23525886. VBISheOne101494_3020. |
Phylogenomic databases | |
| eggNOG | COG0673. |
| HOGENOM | HOG000252553. |
| OMA | TGSWRTY. |
| ProtClustDB | CLSK901939. |
Family and domain databases | |
| Gene3D | 3.40.50.720. 2 hits. |
| InterPro | IPR016040. NAD(P)-bd_dom. IPR000683. Oxidoreductase_N. IPR006311. TAT_signal. IPR019546. TAT_signal_bac_arc. [Graphical view] |
| Pfam | PF01408. GFO_IDH_MocA. 1 hit. PF10518. TAT_signal. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01409. TAT_signal_seq. 1 hit. |
| PROSITE | PS51318. TAT. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GH109_SHEON | ||||||||
| Accession | Primary (citable) accession number: Q8ECL7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |