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Protein

Alpha-N-acetylgalactosaminidase

Gene

nagA

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Glycosidase that has specific alpha-N-acetylgalactosaminidase activity.

Catalytic activityi

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.1 Publication

Cofactori

NAD+By similarityNote: Binds 1 NAD+ per subunit. The NAD+ cannot dissociate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86NADBy similarity1
Binding sitei184NADBy similarity1
Binding sitei213SubstrateBy similarity1
Binding sitei232SubstrateBy similarity1
Binding sitei244NADBy similarity1
Binding sitei326SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi135 – 138NADBy similarity4
Nucleotide bindingi155 – 156NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

NAD

Protein family/group databases

CAZyiGH109. Glycoside Hydrolase Family 109.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
Alternative name(s):
Glycosyl hydrolase family 109 protein
Gene namesi
Name:nagA
Ordered Locus Names:SO_3120
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000008186 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 31Tat-type signalPROSITE-ProRule annotationAdd BLAST31
ChainiPRO_000034856132 – 459Alpha-N-acetylgalactosaminidaseAdd BLAST428

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Proteomic databases

PaxDbiQ8ECL7.

Interactioni

Protein-protein interaction databases

STRINGi211586.SO_3120.

Structurei

3D structure databases

ProteinModelPortaliQ8ECL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni244 – 247Substrate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105EPC. Bacteria.
ENOG410XP5M. LUCA.
HOGENOMiHOG000252553.
OMAiMESGKHA.
OrthoDBiPOG091H0FO5.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ECL7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNIHRRHFL KAAGAVTAGL VTANIALNAN ASSVAPKPSS GKSVIGLIAP
60 70 80 90 100
KMEVVRVGFI GVGERGFSHV EQFCHLEGVE LKAICDTHQA VVDRAVEHIV
110 120 130 140 150
KQKRPKPAVY TGNDLSYREL LNRDDIDIVI ISTPWEWHAP MAIDTMESGK
160 170 180 190 200
HAFVEVPLAL TVEECWQIID TAERTQKNCM MMENVNYGRE ELMVLNMVRQ
210 220 230 240 250
GLFGELLHGE AAYIHELRWQ MKEINHKTGS WRTYWHTKRN GNLYPTHGLG
260 270 280 290 300
PVSQYMNINR GDRFDYLTSM SSPALGRALY AKREFPADHE RNQLKYINGD
310 320 330 340 350
MSTSLIKTVK GRTIMVQHDT TTPRPYSRHN LIQGTNGVFA GFPNRIAVEN
360 370 380 390 400
DGFGTSYHKW DTDMQKWYDK YDHPLWQRIG KEAEINGGHG GMDFVMLWRM
410 420 430 440 450
VYCLRNGEAL DQDVYDGASW SVVNILSEQS LNNRSNSVNF PDFTRGAWEH

AKPLGIVGA
Length:459
Mass (Da):51,965
Last modified:March 1, 2003 - v1
Checksum:i4567186636917ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039445 Genomic DNA. Translation: CAJ01377.1.
AE014299 Genomic DNA. Translation: AAN56125.1.
RefSeqiNP_718681.1. NC_004347.2.
WP_011073016.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN56125; AAN56125; SO_3120.
GeneIDi1170809.
KEGGison:SO_3120.
PATRICi23525886. VBISheOne101494_3020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039445 Genomic DNA. Translation: CAJ01377.1.
AE014299 Genomic DNA. Translation: AAN56125.1.
RefSeqiNP_718681.1. NC_004347.2.
WP_011073016.1. NC_004347.2.

3D structure databases

ProteinModelPortaliQ8ECL7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi211586.SO_3120.

Protein family/group databases

CAZyiGH109. Glycoside Hydrolase Family 109.

Proteomic databases

PaxDbiQ8ECL7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN56125; AAN56125; SO_3120.
GeneIDi1170809.
KEGGison:SO_3120.
PATRICi23525886. VBISheOne101494_3020.

Phylogenomic databases

eggNOGiENOG4105EPC. Bacteria.
ENOG410XP5M. LUCA.
HOGENOMiHOG000252553.
OMAiMESGKHA.
OrthoDBiPOG091H0FO5.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGH109_SHEON
AccessioniPrimary (citable) accession number: Q8ECL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.