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Q8ECL7

- GH109_SHEON

UniProt

Q8ECL7 - GH109_SHEON

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Protein

Alpha-N-acetylgalactosaminidase

Gene

nagA

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Glycosidase that has specific alpha-N-acetylgalactosaminidase activity.

Catalytic activityi

Cleavage of non-reducing alpha-(1->3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.1 Publication

Cofactori

NAD(+)By similarityNote: Binds 1 NAD(+) per subunit. The NAD(+) cannot dissociate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei86 – 861NADBy similarity
Binding sitei184 – 1841NADBy similarity
Binding sitei213 – 2131SubstrateBy similarity
Binding sitei232 – 2321SubstrateBy similarity
Binding sitei244 – 2441NADBy similarity
Binding sitei326 – 3261SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 652NADBy similarity
Nucleotide bindingi135 – 1384NADBy similarity
Nucleotide bindingi155 – 1562NADBy similarity

GO - Molecular functioni

  1. alpha-N-acetylgalactosaminidase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

NAD

Protein family/group databases

CAZyiGH109. Glycoside Hydrolase Family 109.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminidase (EC:3.2.1.49)
Alternative name(s):
Glycosyl hydrolase family 109 protein
Gene namesi
Name:nagA
Ordered Locus Names:SO_3120
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Tat-type signalPROSITE-ProRule annotationAdd
BLAST
Chaini32 – 459428Alpha-N-acetylgalactosaminidasePRO_0000348561Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Protein-protein interaction databases

STRINGi211586.SO_3120.

Structurei

3D structure databases

ProteinModelPortaliQ8ECL7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni244 – 2474Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0673.
HOGENOMiHOG000252553.
OMAiHNIHRRH.
OrthoDBiEOG61P6MK.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8ECL7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHNIHRRHFL KAAGAVTAGL VTANIALNAN ASSVAPKPSS GKSVIGLIAP
60 70 80 90 100
KMEVVRVGFI GVGERGFSHV EQFCHLEGVE LKAICDTHQA VVDRAVEHIV
110 120 130 140 150
KQKRPKPAVY TGNDLSYREL LNRDDIDIVI ISTPWEWHAP MAIDTMESGK
160 170 180 190 200
HAFVEVPLAL TVEECWQIID TAERTQKNCM MMENVNYGRE ELMVLNMVRQ
210 220 230 240 250
GLFGELLHGE AAYIHELRWQ MKEINHKTGS WRTYWHTKRN GNLYPTHGLG
260 270 280 290 300
PVSQYMNINR GDRFDYLTSM SSPALGRALY AKREFPADHE RNQLKYINGD
310 320 330 340 350
MSTSLIKTVK GRTIMVQHDT TTPRPYSRHN LIQGTNGVFA GFPNRIAVEN
360 370 380 390 400
DGFGTSYHKW DTDMQKWYDK YDHPLWQRIG KEAEINGGHG GMDFVMLWRM
410 420 430 440 450
VYCLRNGEAL DQDVYDGASW SVVNILSEQS LNNRSNSVNF PDFTRGAWEH

AKPLGIVGA
Length:459
Mass (Da):51,965
Last modified:March 1, 2003 - v1
Checksum:i4567186636917ADF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039445 Genomic DNA. Translation: CAJ01377.1.
AE014299 Genomic DNA. Translation: AAN56125.1.
RefSeqiNP_718681.1. NC_004347.2.
WP_011073016.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN56125; AAN56125; SO_3120.
GeneIDi1170809.
KEGGison:SO_3120.
PATRICi23525886. VBISheOne101494_3020.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039445 Genomic DNA. Translation: CAJ01377.1 .
AE014299 Genomic DNA. Translation: AAN56125.1 .
RefSeqi NP_718681.1. NC_004347.2.
WP_011073016.1. NC_004347.2.

3D structure databases

ProteinModelPortali Q8ECL7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211586.SO_3120.

Protein family/group databases

CAZyi GH109. Glycoside Hydrolase Family 109.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN56125 ; AAN56125 ; SO_3120 .
GeneIDi 1170809.
KEGGi son:SO_3120.
PATRICi 23525886. VBISheOne101494_3020.

Phylogenomic databases

eggNOGi COG0673.
HOGENOMi HOG000252553.
OMAi HNIHRRH.
OrthoDBi EOG61P6MK.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR016040. NAD(P)-bd_dom.
IPR000683. Oxidoreductase_N.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF01408. GFO_IDH_MocA. 1 hit.
PF10518. TAT_signal. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME ACTIVITY.
    Strain: ATCC 70050.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiGH109_SHEON
AccessioniPrimary (citable) accession number: Q8ECL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: March 1, 2003
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3