Q8ECD7 (CHEB1_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon
EC=3.1.1.61

Gene names

Name:	cheB1
Synonyms:	cheB3
Ordered Locus Names:	SO_3206

Organism

Shewanella oneidensis

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Chemotaxis response regulator protein-glutamate methylesterase of group 1 operon HAMAP MF_00099		PRO_0000158028
Regions
	Domain	4 – 121	118	Response regulatory
	Domain	174 – 374	201	CheB-type methylesterase
Sites
	Active site	193	1	By similarity
	Active site	220	1	By similarity
	Active site	316	1	By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8ECD7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8F58AC58392B3D0B	FASTA	374	39,484
	10 20 30 40 50 60 MAIKVLVVDD SSFFRRRVSE IVNQDPELEV IATASNGAEA VKMTADLNPQ VITMDIEMPV 70 80 90 100 110 120 MDGITAVREI MAKCPTPILM FSSLTHDGAK ATLDALDAGA LDFLPKRFED IATNKDEAIL 130 140 150 160 170 180 LLQQRVKALG RRRIFRPITR PSVAPVTPRP TTGSAVGNAT TPVQSASAPV RSSPLSSIRA 190 200 210 220 230 240 SGKQYKLLLI GTSTGGPVAL QKVLTQFPAN YPHPILLIQH MPAAFTPAFA SRLNGLCKIE 250 260 270 280 290 300 VKEAANGDIL RPGCAYLAPG GMQMMVERAG VTGRIKVLAG SAEMNYKPCV DITFASASKA 310 320 330 340 350 360 YGGDVLAVVL TGMGADGREG ARMLKAAGAT IWAQDEASCV VYGMPQAVAS TGIATQSISL 370 DNMAESILKE SARG

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN56205.1.
RefSeq	NP_718761.1. NC_004347.1.
3D structure databases
ProteinModelPortal	Q8ECD7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1170899.
GenomeReviews	Gene locus SO_3206 in contig AE014299_GR.
KEGG	son:SO_3206.
NMPDR	fig|211586.1.peg.2924.
PATRIC	23526096. VBISheOne101494_3112.
TIGR	SO_3206.
Phylogenomic databases
HOGENOM	HBG705324.
OMA	KMAAELN.
ProtClustDB	PRK00742.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3206-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_SHEON

Accession

Primary (citable) accession number: Q8ECD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program