Reviewed, UniProtKB/Swiss-Prot Q8EC57 (PUR4_SHEON)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	SO_3287

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	1293 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1293	1293	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100419
Regions
	Domain	1040 – 1293	254	Glutamine amidotransferase type-1
	Nucleotide binding	305 – 316	12	ATP Potential
Sites
	Active site	1133	1	Nucleophile By similarity
	Active site	1258	1	By similarity
	Active site	1260	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EC57-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6F4E26B7A350CB05	FASTA	1,293	140,598
	10 20 30 40 50 60 MEIIRGAPAL STFRVQKLME ACVSAALPVR QIYAEYVHLA DLSELLKPTE REQLEKILTY 70 80 90 100 110 120 GPAIEAHTPQ GSLLFVTPRP GTISPWSSKA TDIAHNCGLG KVKRLERGIA YYVESDTLTA 130 140 150 160 170 180 EQQRTLQGLL HDRMVEVVLN DFAKADVLFK RTEPAPFKSV NVLAEGRRAL EVANVEMGLA 190 200 210 220 230 240 LAEDEIDYLV ENFVRLNRNP NDIELMMFAQ ANSEHCRHKI FNADWTIDGK AQPKSLFKMI 250 260 270 280 290 300 KNTFEVTPDH VLSAYKDNAA VMEGSVAGRF FPDPNGVYSY HTEPMHVLMK VETHNHPTAI 310 320 330 340 350 360 SPYPGAATGS GGEIRDEGAT GRGSKPKAGL TGFSVSNLKI PGFVQPWEGN YGKPDRIVSA 370 380 390 400 410 420 LDIMTEGPLG GAAFNNEFGR PALLGYFRTY EQEVSSHNGV EMRGYHKPIM LAGGLGNIRE 430 440 450 460 470 480 EHVQKGEITV GAKLIVLGGP AMNIGLGGGA ASSMASGQSS EDLDFASVQR ENPEMERRCQ 490 500 510 520 530 540 EVIDRCWQLG DKNPIQFIHD VGAGGLSNAF PELVNDGDRG GIFNLRNVPS DEPGMSPLEI 550 560 570 580 590 600 WCNESQERYV LSVAAEDLPL FTAICERERA PFAVVGEAIQ EQHLTLADSH FDNNPIDLPL 610 620 630 640 650 660 EVLLGKAPKM SRNVVSAKAV SPALEQSQID VKDAVKRVLS LPTVADKTFL ITIGDRTVTG 670 680 690 700 710 720 LVNRDQMVGP WQVPVADCAV TAASFDTYAG EAMSLGERTP LALLDFGASA RMAVAESIMN 730 740 750 760 770 780 IAGADIGSFK RIKLSANWMS AAGHPGEDAG LYEAVKAVGE ELCPELSLTI PVGKDSMSMK 790 800 810 820 830 840 TAWQQDGVNK TVTSPMSLVI TAFGVVQDIR NTVTPELRSD KGETSLLLVD LGAGQNRLGG 850 860 870 880 890 900 SCLAQVYGEL GDVAPDLDDA ALLRGFFETM QKLVANKLVI AYHDRSDGGL FTTLVEMAFA 910 920 930 940 950 960 GNIGLDIDVE DLQGTDLERL FNEELGAVLQ VSRDNAAKIA AQFAIAGVPC HVIGTLADDQ 970 980 990 1000 1010 1020 CITIKDGARE IFSDTRVALR TVWSETTYRM QAMRDNPACA LEEFKLKQDE TDLGLTVNLS 1030 1040 1050 1060 1070 1080 FDPSEDVAAP YILKGAAPKM AILREQGVNS HVEMAAAFDR AGFESRDVHM SDILSGRISL 1090 1100 1110 1120 1130 1140 EEFQGLVACG GFSYGDVLGA GEGWAKSILF NERARNEFSR FFERDSSFAL GVCNGCQMLS 1150 1160 1170 1180 1190 1200 NLKEIIPGSE HWPRFVRNRS ERFEARFSLV EVQQSPSLFF QGMAGSRMPI AVSHGEGHAE 1210 1220 1230 1240 1250 1260 FASAQALALA EASGTIALRF VNGKGEIATQ YPQNPNGSPN GLTGICTTDG RVTLMMPHPE 1270 1280 1290 RVFRTVANSW HPDNWGEDSP WMRMFRNARV NLG

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN56285.1.
RefSeq	NP_718841.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1170977.
GenomeReviews	Gene locus SO_3287 in contig AE014299_GR.
KEGG	son:SO_3287.
NMPDR	fig|211586.1.peg.3001.
TIGR	SO_3287.
Phylogenomic databases
HOGENOM	HBG335309.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3287-MONOMER.
BRENDA	6.3.5.3. 257422.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_SHEON

Accession

Primary (citable) accession number: Q8EC57

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)