Reviewed, UniProtKB/Swiss-Prot Q8EBZ7 (HEMH2_SHEON)

Names and origin

Protein names

Recommended name:
    Ferrochelatase 2
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase 2
    Heme synthetase 2

Gene names

Name:	hemH2
Synonyms:	hemH-2
Ordered Locus Names:	SO_3348

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Ferrochelatase 2 HAMAP MF_00323		PRO_0000175199
Sites
	Metal binding	201	1	Iron By similarity
	Metal binding	282	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EBZ7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D098A1901F24171A	FASTA	327	36,398
	10 20 30 40 50 60 MGHAARGKVG VLLLNLGTPD APTASAVRRY LAEFLSDPRV VEIPKLLWML ILYGIVLRVR 70 80 90 100 110 120 PAKSAALYQK VWTEAGSPLM DISLRQTAKL SDKLTADGHQ VSVHLAMRYG NPSVASTLRE 130 140 150 160 170 180 MHKQGIDKLV VLPLYPQYAA PTTGSAFDAI AKELSQWRYL PSLHFINTYH DNPDFIAALV 190 200 210 220 230 240 NSIRDDFDKH GKPQKLVLSY HGMPERNLHL GDPYYCFCMK TTRLVAEQLG LSKDEFAITF 250 260 270 280 290 300 QSRFGKAKWL QPYTDATMAA LPSQGVRDVA IVCPAFSADC LETLEEIVGE NGHIFTHAGG 310 320 EKFRYIPALN DNDDHIAMMA NLVKPYL

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN56346.1.
RefSeq	NP_718902.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1171030.
GenomeReviews	Gene locus SO_3348 in contig AE014299_GR.
KEGG	son:SO_3348.
NMPDR	fig|211586.1.peg.3054.
TIGR	SO_3348.
Phylogenomic databases
HOGENOM	Q8EBZ7.
OMA	HIFTEAG.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3348-MON.
BRENDA	4.99.1.1. 257422.
Family and domain databases
HAMAP	MF_00323. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
ProDom	PD002792. Ferrochelatase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH2_SHEON

Accession

Primary (citable) accession number: Q8EBZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)