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Q8EBI4

- SYI_SHEON

UniProt

Q8EBI4 - SYI_SHEON

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Protein

Isoleucine--tRNA ligase

Gene
ileS, SO_3532
Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei564 – 5641Aminoacyl-adenylate By similarity
Binding sitei608 – 6081ATP By similarity
Metal bindingi903 – 9031Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi923 – 9231Zinc By similarity
Metal bindingi926 – 9261Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SO_3532
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligaseUniRule annotationPRO_0000098459Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi211586.SO_3532.

Structurei

3D structure databases

ProteinModelPortaliQ8EBI4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi605 – 6095"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.
PhylomeDBiQ8EBI4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EBI4-1 [UniParc]FASTAAdd to Basket

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MSDYKFTLNL PETEFPMRGN LANREPEMLE RWTKDGLYQQ IRDSRIGRTP    50
FILHDGPPYA NGSIHIGHSV NKILKDIIVK SKTMSGFDAP YVPGWDCHGL 100
PIELKVEQKV GKPGQKISAA EFREECRKYA AEQVDGQRED FIRLGVLGDW 150
QNPYLTMDFA TEANIVRSLS KVIENGHLHK GVKPVHWCTD CGSALAEAEV 200
EYEDKTSPAI DVAFTAVDSK AVAVKFGVSD YSHSVSMVIW TTTPWTLPAN 250
RALSISPELD YSLVEFVKDG VTHAVILADV LVEACMTRYG AESHSVLAKI 300
KGAALELVRF KHPFLAFDVP AILGDHVTTD AGTGVVHTAP GHGQDDFVVG 350
QKYGLEVANP VGDNGVYKPD TEFFAGQHVF KANDNVVALL KEKGALLHHV 400
AYRHSYPHCW RHKTPIIFRA TPQWFISMDN HNLRKQALSE IEQIQWIPDW 450
GQSRIEKMVE NRPDWCISRQ RTWGVPITLF VHRETEELHP DSVSLMARVA 500
NRIEQEGIQA WWDLDAAELL GEEAEQYRKV TDTLDVWYDS GSTFASVVAA 550
RPEFHGHGVD LYLEGSDQHR GWFMSSLMIS TAMTGKAPYK QVLTHGFTVD 600
GKGRKMSKSI GNVIAPQQVT NKLGADILRL WVAATDYSGE MTVSDEILNR 650
SADAYRRIRN TARFLLANLN GFDPKNDLVA VEDMVALDRW AVRRAAALQQ 700
EIIEAYEQYN FHIVTQKLMQ FCSIELGSFY LDIIKDRQYT AKQEGHARRS 750
CQSALFHIAE AMVRWIAPVL SFTADEVWQL LPGQRDAYVF TQEWYQGLQS 800
ITLDTDLSDA YWENLLTVRN EVNKVIEQAR RDKRVGGSLE AEVTLFADAT 850
LTEQLTHIGD ELRFVLLTSE AKVLPLVDAT SDAVETELAS LKLVVNATTA 900
EKCERCWHHR EEVGTIEAHP TLCHRCVTNI EGDGEVRLFA 940
Length:940
Mass (Da):105,792
Last modified:March 1, 2003 - v1
Checksum:iEC7CADE3F3DE8FA8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN56523.1.
RefSeqiNP_719079.1. NC_004347.2.
WP_011073365.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN56523; AAN56523; SO_3532.
GeneIDi1171205.
KEGGison:SO_3532.
PATRICi23526754. VBISheOne101494_3428.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN56523.1 .
RefSeqi NP_719079.1. NC_004347.2.
WP_011073365.1. NC_004347.2.

3D structure databases

ProteinModelPortali Q8EBI4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 211586.SO_3532.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN56523 ; AAN56523 ; SO_3532 .
GeneIDi 1171205.
KEGGi son:SO_3532.
PATRICi 23526754. VBISheOne101494_3428.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.
PhylomeDBi Q8EBI4.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiSYI_SHEON
AccessioniPrimary (citable) accession number: Q8EBI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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