Q8EB78 (UNG_SHEON) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	SO_3654

Organism

Shewanella oneidensis (strain MR-1) [Reference proteome] [HAMAP]

Taxonomic identifier

211586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella › Shewanella oneidensis

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_0000176135
Sites
	Active site	60	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EB78 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 751FF4DC183851B3	FASTA	218	24,434
	10 20 30 40 50 60 MTWPAFIDHQ RTQPYYQQLI AFVNQERQVG KVIYPPKEDV FNAFKTTPLE QVRVVLIGQD 70 80 90 100 110 120 PYHGPDQAHG LCFSVKRGVK APPSLANMYK ELVNDIPGFH IPNHGDLTQW AEQGILMLNT 130 140 150 160 170 180 VLTVEQGQAH SHANAGWEIF TTEALKLLNA QERPIIFVLW GSHAIKKGAV ITAPQHQILS 190 200 210 GPHPSPLSAY RGFFGCGHFS KVNQLLMARG EAPIQWQI

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014299 Genomic DNA. Translation: AAN56640.1.
RefSeq	NP_719196.1. NC_004347.2.
3D structure databases
ProteinModelPortal	Q8EB78.
SMR	Q8EB78. Positions 1-217.
ModBase	Search...
Protein-protein interaction databases
STRING	211586.SO_3654.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN56640; AAN56640; SO_3654.
GeneID	1171313.
KEGG	son:SO_3654.
PATRIC	23526994. VBISheOne101494_3542.
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	AGKEIYP.
ProtClustDB	PRK05254.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_SHEON

Accession

Primary (citable) accession number: Q8EB78

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program