ID   CYSI_SHEON              Reviewed;         565 AA.
AC   Q8EB00;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=Sulfite reductase [NADPH] hemoprotein beta-component;
DE            Short=SIR-HP;
DE            Short=SIRHP;
DE            EC=1.8.1.2;
GN   Name=cysI; OrderedLocusNames=SO_3737;
OS   Shewanella oneidensis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=70863;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MR-1;
RX   MEDLINE=22297686; PubMed=12368813; DOI=10.1038/nbt749;
RA   Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C.,
RA   Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A.,
RA   Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J.,
RA   Brinkac L.M., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S.,
RA   Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A.,
RA   White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M.,
RA   Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J.,
RA   Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O.,
RA   Venter J.C., Nealson K.H., Fraser C.M.;
RT   "Genome sequence of the dissimilatory metal ion-reducing bacterium
RT   Shewanella oneidensis.";
RL   Nat. Biotechnol. 20:1118-1123(2002).
CC   -!- FUNCTION: This enzyme catalyzes the 6-electron reduction of
CC       sulfite to sulfide. This is one of several activities required for
CC       the biosynthesis of L-cysteine from sulfate (By similarity).
CC   -!- CATALYTIC ACTIVITY: H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3
CC       NADPH.
CC   -!- COFACTOR: Binds 1 siroheme per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
CC   -!- SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein,
CC       the beta component is a hemoprotein (By similarity).
CC   -!- SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S
CC       domain family.
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DR   EMBL; AE014299; AAN56720.1; -; Genomic_DNA.
DR   RefSeq; NP_719276.1; -.
DR   HSSP; P17846; 1AOP.
DR   GeneID; 1171387; -.
DR   GenomeReviews; AE014299_GR; SO_3737.
DR   KEGG; son:SO_3737; -.
DR   NMPDR; fig|211586.1.peg.3396; -.
DR   TIGR; SO_3737; -.
DR   HOGENOM; Q8EB00; -.
DR   OMA; Q8EB00; ITTTQWQ.
DR   BioCyc; SONE211586:SO_3737-MON; -.
DR   BRENDA; 1.8.1.2; 257422.
DR   GO; GO:0009337; C:sulfite reductase complex (NADPH); IEA:InterPro.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0004783; F:sulfite reductase (NADPH) activity; IEA:HAMAP.
DR   GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0000103; P:sulfate assimilation; IEA:HAMAP.
DR   HAMAP; MF_01540; -; 1.
DR   InterPro; IPR011786; CysI.
DR   InterPro; IPR006066; Nir_Si_BS.
DR   InterPro; IPR006067; Nir_Sir_4Fe4S.
DR   InterPro; IPR005117; NiRdtase/SiRdtase_haem-b_fer.
DR   Pfam; PF01077; NIR_SIR; 1.
DR   Pfam; PF03460; NIR_SIR_ferr; 2.
DR   PRINTS; PR00397; SIROHAEM.
DR   TIGRFAMs; TIGR02041; CysI; 1.
DR   PROSITE; PS00365; NIR_SIR; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Amino-acid biosynthesis; Complete proteome;
KW   Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP;
KW   Oxidoreductase.
FT   CHAIN         1    565       Sulfite reductase [NADPH] hemoprotein
FT                                beta-component.
FT                                /FTId=PRO_0000199909.
FT   METAL       429    429       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       435    435       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       474    474       Iron-sulfur (4Fe-4S) (By similarity).
FT   METAL       478    478       Iron (siroheme axial ligand) (By
FT                                similarity).
FT   METAL       478    478       Iron-sulfur (4Fe-4S) (By similarity).
SQ   SEQUENCE   565 AA;  63063 MW;  420F3290EE85066C CRC64;
     MSEQKLALNE YLKTDSDYLR GTIKEGLDSS VTGSFSDGDQ QLIKFHGFYQ QDDRDLRNER
     KEQKLEPLYS FMLRARVPGG VCTPKQWLGV DEIASTLTSS NSIRLTTRQT FQYHGIPKRN
     LKTIIQGLDR EALDSIAACG DVNRNVMCNP NPVESKLHAQ AYEVAKKLSD HLLPHTRAYA
     EIWLDEEKLL TTEDETVEPV YGKTYLPRKF KMAVAVPPDN DVDVYTNDLG FIAVAENGEL
     VGFNLTAGGG MGSTHGEVET FPRLADDFGF IKTEDVMKFA EAVMTVQRDW GNRTNRKRSR
     LKYTIVDHGY EKFKAEVEVR AGVKFEPKRD VVIGDRGDRY GWVEGVDGKW HLTLFIESGR
     IKDMPGKSLQ TGMREIAKIH KGDFRMTSNQ NMIIAGVAPE DKATIEGLAR KRGLLGQVLT
     QTRGHSIACV ALPTCPLAMA EAERYFPEFI DHIDALQAKN GISDQAIVVR MTGCPNGCAR
     PFAAEIGLVG KAPGRYNLYL GANFEGTRLN KMYRENIQEA EILAELDALF ARYAIERNAG
     ETFGNFTVRV GVVKAVIDAA KDFHG
//