Reviewed, UniProtKB/Swiss-Prot Q8EB00 (CYSI_SHEON)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	SO_3737

Organism

Shewanella oneidensis [Complete proteome] [HAMAP]

Taxonomic identifier

70863 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_0000199909
Sites
	Metal binding	429	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	474	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	478	1	Iron (siroheme axial ligand) By similarity
	Metal binding	478	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8EB00-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 420F3290EE85066C	FASTA	565	63,063
	10 20 30 40 50 60 MSEQKLALNE YLKTDSDYLR GTIKEGLDSS VTGSFSDGDQ QLIKFHGFYQ QDDRDLRNER 70 80 90 100 110 120 KEQKLEPLYS FMLRARVPGG VCTPKQWLGV DEIASTLTSS NSIRLTTRQT FQYHGIPKRN 130 140 150 160 170 180 LKTIIQGLDR EALDSIAACG DVNRNVMCNP NPVESKLHAQ AYEVAKKLSD HLLPHTRAYA 190 200 210 220 230 240 EIWLDEEKLL TTEDETVEPV YGKTYLPRKF KMAVAVPPDN DVDVYTNDLG FIAVAENGEL 250 260 270 280 290 300 VGFNLTAGGG MGSTHGEVET FPRLADDFGF IKTEDVMKFA EAVMTVQRDW GNRTNRKRSR 310 320 330 340 350 360 LKYTIVDHGY EKFKAEVEVR AGVKFEPKRD VVIGDRGDRY GWVEGVDGKW HLTLFIESGR 370 380 390 400 410 420 IKDMPGKSLQ TGMREIAKIH KGDFRMTSNQ NMIIAGVAPE DKATIEGLAR KRGLLGQVLT 430 440 450 460 470 480 QTRGHSIACV ALPTCPLAMA EAERYFPEFI DHIDALQAKN GISDQAIVVR MTGCPNGCAR 490 500 510 520 530 540 PFAAEIGLVG KAPGRYNLYL GANFEGTRLN KMYRENIQEA EILAELDALF ARYAIERNAG 550 560 ETFGNFTVRV GVVKAVIDAA KDFHG

References

[1]

"Genome sequence of the dissimilatory metal ion-reducing bacterium Shewanella oneidensis." Heidelberg J.F., Paulsen I.T., Nelson K.E., Gaidos E.J., Nelson W.C., Read T.D., Eisen J.A., Seshadri R., Ward N.L., Methe B.A., Clayton R.A., Meyer T., Tsapin A., Scott J., Beanan M.J., Brinkac L.M., Daugherty S.C., DeBoy R.T. , Dodson R.J., Durkin A.S., Haft D.H., Kolonay J.F., Madupu R., Peterson J.D., Umayam L.A., White O., Wolf A.M., Vamathevan J.J., Weidman J.F., Impraim M., Lee K., Berry K.J., Lee C., Mueller J., Khouri H.M., Gill J., Utterback T.R., McDonald L.A., Feldblyum T.V., Smith H.O., Venter J.C., Nealson K.H., Fraser C.M. Nat. Biotechnol. 20:1118-1123(2002) [PubMed: 12368813] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MR-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014299 Genomic DNA. Translation: AAN56720.1.
RefSeq	NP_719276.1.
3D structure databases
HSSP	HSSP built from PDB template 1AOP based on UniProtKB P17846.
ModBase	Search...
Genome annotation databases
GeneID	1171387.
GenomeReviews	Gene locus SO_3737 in contig AE014299_GR.
KEGG	son:SO_3737.
NMPDR	fig|211586.1.peg.3396.
TIGR	SO_3737.
Phylogenomic databases
HOGENOM	Q8EB00.
OMA	Q8EB00. ITTTQWQ.
Enzyme and pathway databases
BioCyc	SONE211586:SO_3737-MON.
BRENDA	1.8.1.2. 257422.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SHEON

Accession

Primary (citable) accession number: Q8EB00

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)