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Q8EAZ9

- CYSJ_SHEON

UniProt

Q8EAZ9 - CYSJ_SHEON

Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 FAD per subunit.UniRule annotation
    Binds 1 FMN per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei497 – 4971NADPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 765FMNUniRule annotation
    Nucleotide bindingi119 – 1246FMNUniRule annotation
    Nucleotide bindingi152 – 18332FMNUniRule annotationAdd
    BLAST
    Nucleotide bindingi395 – 3984FADUniRule annotation
    Nucleotide bindingi429 – 4313FADUniRule annotation
    Nucleotide bindingi527 – 5359NADPUniRule annotation

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. sulfite reductase (NADPH) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
    3. sulfate assimilation Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-FPUniRule annotation
    Gene namesi
    Name:cysJUniRule annotation
    Ordered Locus Names:SO_3738
    OrganismiShewanella oneidensis (strain MR-1)
    Taxonomic identifieri211586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
    ProteomesiUP000008186: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 607607Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199936Add
    BLAST

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Protein-protein interaction databases

    STRINGi211586.SO_3738.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8EAZ9.
    SMRiQ8EAZ9. Positions 234-607.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 204139Flavodoxin-likeUniRule annotationAdd
    BLAST
    Domaini239 – 456218FAD-binding FR-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.UniRule annotation
    Contains 1 flavodoxin-like domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0369.
    HOGENOMiHOG000282025.
    KOiK00380.
    OMAiHEFLQSK.
    OrthoDBiEOG6CVV7G.
    PhylomeDBiQ8EAZ9.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_01541. CysJ.
    InterProiIPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsiTIGR01931. cysJ. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8EAZ9-1 [UniParc]FASTAAdd to Basket

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    MLLKELSSLA SPLSQSQVDK LKQLTAELNS VQLAWVSGYL AATANTSGSA    50
    IQVAASVTEA QAAQTVTILY GSQTGNGRGI AKALAEKAKT QGYSVNLASM 100
    GEYNVRQLKQ ETLLLLVVST HGEGEAPDDA IELHKFLATK RAPQLNNLHY 150
    SVLALGDSSY EFFCQTGKDF DARLSALGAK ALLPLVECDV DYEAAAGQWH 200
    ADVLTAVKPL IQTTANVVAL NEINSTSAQV ASESEFTKQN PYRAEVLVSQ 250
    KITGRDSDRD VRHVEIDLGE SGLHYEVGDA LGVWFSNSEI LVGEILAGLG 300
    LAADAKVTVG SESISLKQAL IDKKELTQLY PGLVKAWAEL SASSELLALS 350
    EDKEQLRQFI LNHQFVDLVT NYKLPAEANL DANKLLELLR PLTPRLYSIA 400
    SSQTEVDTEV HLTVALVEDE HQGQTRFGGA SHFLASAQEG AEVKVYVEPN 450
    KHFRLPENPD TPVIMIGPGT GVAPFRAFMQ ERVAQGAKGD SWLFFGNPHF 500
    EQDFLYQTEW QQYLKNGDLT RIDVAFSRDQ AHKIYVQHRI KEQGQALWQW 550
    LQNGAHLYIC GDAERMAKDV HQALLAVAVE FGGLSSEAAE EYFETLRSHK 600
    RYQKDVY 607
    Length:607
    Mass (Da):66,620
    Last modified:March 1, 2003 - v1
    Checksum:iC6042663547ACD22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN56721.1.
    RefSeqiNP_719277.1. NC_004347.2.

    Genome annotation databases

    EnsemblBacteriaiAAN56721; AAN56721; SO_3738.
    GeneIDi1171388.
    KEGGison:SO_3738.
    PATRICi23527152. VBISheOne101494_3621.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014299 Genomic DNA. Translation: AAN56721.1 .
    RefSeqi NP_719277.1. NC_004347.2.

    3D structure databases

    ProteinModelPortali Q8EAZ9.
    SMRi Q8EAZ9. Positions 234-607.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 211586.SO_3738.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN56721 ; AAN56721 ; SO_3738 .
    GeneIDi 1171388.
    KEGGi son:SO_3738.
    PATRICi 23527152. VBISheOne101494_3621.

    Phylogenomic databases

    eggNOGi COG0369.
    HOGENOMi HOG000282025.
    KOi K00380.
    OMAi HEFLQSK.
    OrthoDBi EOG6CVV7G.
    PhylomeDBi Q8EAZ9.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPi MF_01541. CysJ.
    InterProi IPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsi TIGR01931. cysJ. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MR-1.

    Entry informationi

    Entry nameiCYSJ_SHEON
    AccessioniPrimary (citable) accession number: Q8EAZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 13, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3