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Q8EAZ9

- CYSJ_SHEON

UniProt

Q8EAZ9 - CYSJ_SHEON

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Protein
Sulfite reductase [NADPH] flavoprotein alpha-component
Gene
cysJ, SO_3738
Organism
Shewanella oneidensis (strain MR-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Binds 1 FAD per subunit By similarity.UniRule annotation
Binds 1 FMN per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei497 – 4971NADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi72 – 765FMN By similarity
Nucleotide bindingi119 – 1246FMN By similarity
Nucleotide bindingi152 – 18332FMN By similarity
Add
BLAST
Nucleotide bindingi395 – 3984FAD By similarity
Nucleotide bindingi429 – 4313FAD By similarity
Nucleotide bindingi527 – 5359NADP By similarity

GO - Molecular functioni

  1. FMN binding Source: InterPro
  2. flavin adenine dinucleotide binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component (EC:1.8.1.2)
Short name:
SiR-FP
Gene namesi
Name:cysJ
Ordered Locus Names:SO_3738
OrganismiShewanella oneidensis (strain MR-1)
Taxonomic identifieri211586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
ProteomesiUP000008186: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 607607Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation
PRO_0000199936Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Protein-protein interaction databases

STRINGi211586.SO_3738.

Structurei

3D structure databases

ProteinModelPortaliQ8EAZ9.
SMRiQ8EAZ9. Positions 234-607.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini66 – 204139Flavodoxin-like
Add
BLAST
Domaini239 – 456218FAD-binding FR-type
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282025.
KOiK00380.
OMAiHEFLQSK.
OrthoDBiEOG6CVV7G.
PhylomeDBiQ8EAZ9.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8EAZ9-1 [UniParc]FASTAAdd to Basket

« Hide

MLLKELSSLA SPLSQSQVDK LKQLTAELNS VQLAWVSGYL AATANTSGSA    50
IQVAASVTEA QAAQTVTILY GSQTGNGRGI AKALAEKAKT QGYSVNLASM 100
GEYNVRQLKQ ETLLLLVVST HGEGEAPDDA IELHKFLATK RAPQLNNLHY 150
SVLALGDSSY EFFCQTGKDF DARLSALGAK ALLPLVECDV DYEAAAGQWH 200
ADVLTAVKPL IQTTANVVAL NEINSTSAQV ASESEFTKQN PYRAEVLVSQ 250
KITGRDSDRD VRHVEIDLGE SGLHYEVGDA LGVWFSNSEI LVGEILAGLG 300
LAADAKVTVG SESISLKQAL IDKKELTQLY PGLVKAWAEL SASSELLALS 350
EDKEQLRQFI LNHQFVDLVT NYKLPAEANL DANKLLELLR PLTPRLYSIA 400
SSQTEVDTEV HLTVALVEDE HQGQTRFGGA SHFLASAQEG AEVKVYVEPN 450
KHFRLPENPD TPVIMIGPGT GVAPFRAFMQ ERVAQGAKGD SWLFFGNPHF 500
EQDFLYQTEW QQYLKNGDLT RIDVAFSRDQ AHKIYVQHRI KEQGQALWQW 550
LQNGAHLYIC GDAERMAKDV HQALLAVAVE FGGLSSEAAE EYFETLRSHK 600
RYQKDVY 607
Length:607
Mass (Da):66,620
Last modified:March 1, 2003 - v1
Checksum:iC6042663547ACD22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN56721.1.
RefSeqiNP_719277.1. NC_004347.2.

Genome annotation databases

EnsemblBacteriaiAAN56721; AAN56721; SO_3738.
GeneIDi1171388.
KEGGison:SO_3738.
PATRICi23527152. VBISheOne101494_3621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014299 Genomic DNA. Translation: AAN56721.1 .
RefSeqi NP_719277.1. NC_004347.2.

3D structure databases

ProteinModelPortali Q8EAZ9.
SMRi Q8EAZ9. Positions 234-607.
ModBasei Search...

Protein-protein interaction databases

STRINGi 211586.SO_3738.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN56721 ; AAN56721 ; SO_3738 .
GeneIDi 1171388.
KEGGi son:SO_3738.
PATRICi 23527152. VBISheOne101494_3621.

Phylogenomic databases

eggNOGi COG0369.
HOGENOMi HOG000282025.
KOi K00380.
OMAi HEFLQSK.
OrthoDBi EOG6CVV7G.
PhylomeDBi Q8EAZ9.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPi MF_01541. CysJ.
InterProi IPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsi TIGR01931. cysJ. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MR-1.

Entry informationi

Entry nameiCYSJ_SHEON
AccessioniPrimary (citable) accession number: Q8EAZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: March 1, 2003
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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